Embryologists team vs. automated annotation software outcomes

Abstract

OBJECTIVE: To compare clinical outcomes after fresh embryo transfer on laser-assisted zona pellucida opening (LAO) versus thinning (LAT) according to maternal age in patients with repeated implantation failure (RIF).DESIGN: A retrospective study of 509 (n ¼ 458 patients) in vitro fertilization/intracytoplasmic sperm injection cycles was investigated from January 2013 to July 2017. MATERIALS AND METHODS: We compared whether LAT and LAO affect the clinical outcomes in young maternal age (YMA, <38 years) and old maternal age (OMA, R38 years) patient groups with R2 of RIF. The cycles with an oocyte donation, oocyte activation, genetic diagnosis, and that used surrogate mothers were excluded. Participants were divided into 4 groups according to maternal age and the two types of laser-assisted hatching (YMA: LAT, n ¼ 119 vs. LAO, n ¼ 179 and OMA: LAT, n ¼ 72 vs. LAO, n ¼ 139). LAO was opened using 3-4 laser shot in the zona pellucida. The laser thinning was performed by making 3-4 holes without reaching the inner membrane at a depth of 60%-80% of the zona pellucida thickness. Laser-assisted hatching was performed 2 hours before the embryo transfer.RESULTS: The characteristics of patients did not differ significantly among the groups (p > 0.05), with the exception of mixed factor infertility, which was more common in the LAT group than in the LAO group among patients <38 years of age (10.1% vs. 2.8%, p ¼ 0.008). We also observed similar rates of clinical pregnancy (27.7% vs. twin pregnancy (5.0% vs. 5.6%, p ¼ 0.498; 0.0% vs. 2.2, p ¼ 0.553) between LAT and LAO in the YMA or the OMA group. CONCLUSIONS: Clinical outcomes were similar between LAT and LAO in the YMA or the OMA group. However, the OMA group who underwent LAO tended to have a lower abortion rate. Further study is necessary to confirm these results in a larger population. P-274 Tuesday, October 9, 2018 6:30 AM PIEZO-ICSI AS ALTERNATIVE TOOL TO IMPROVE OOCYTE ACTIVATION IN IN VITRO MATURED BOVINE OOCYTES MODEL.OBJECTIVE: Evaluate the fertilization rates in a bovine oocyte activation failure model of Piezo-ICSI compared to conventional ICSI. DESIGN: We aimed to evaluate the participation of Piezo-pulse and sperm species in oocyte activation. In vitro matured bovine oocytes were randomly allocated to one of four groups: 1-conventional ICSI with bull sperm, 2-Piezo-ICSI with bull sperm, 3-conventional ICSI with human sperm, 4-Piezo-ICSI with human sperm.MATERIALS AND METHODS: Cumulus-oocyte complexes were obtained from bovine ovaries from slaughterhouse and matured in vitro. Cryopreserved sperm from bulls and humans with proven fertility were used. Conventional ICSI was performed with bevelled spiked micropipettes. Piezo-ICSI was performed with flat-tipped micropipettes. Fertilization rates were evaluated 18 h after ICSI by fixing and staining the presumptive zygotes with Hoechst 33342. Cleavage rates were evaluated 48 h after ICSI.RESULTS: Fertilization rate (2 pn) was higher with Piezo-ICSI (22.3%) than conventional ICSI (5.9%) using bull sperm (P<0.05). The sam...