Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve

Báez, J. C.; Gajavelli, Shyam; Thomas, Christine K.; Grumbles, Robert M.; Aparicio, Beatriz; Byer, David E.; Tsoulfas, Pantelis

doi:10.1016/j.expneurol.2004.06.013

Cited by 10 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the local environment is not suitable for the survival of NSCs after acute SCI, more strategies should be considered to protect NSCs and to improve their survival after transplantation to promote functional recovery. Since peripheral nerve transplantation for the treatment of SCI had been studied by many authors, and since transplantation of NSCs has produced some interesting results in the treatment of peripheral nerve injury [11][12][13][14] , it is possible that peripheral nerve could serve as a vehicle to host NSCs and that transplantation of peripheral nerve with NSCs may have some effects in the repair of damaged spinal cord.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of a Peripheral Nerve with Neural Stem Cells Plus Lithium Chloride Injection Promote the Recovery of Rat Spinal Cord Injury

Zhang

Deng

et al. 2018

Cell Transplant

View full text Add to dashboard Cite

Transplantation of neural stem cells (NSCs) holds great potential for the treatment of spinal cord injury (SCI). However, transplanted NSCs poorly survive in the SCI environment. We injected NSCs into tibial nerve and transplanted tibial nerve into a hemisected spinal cord and investigated the effects of lithium chloride (LiCl) on the survival of spinal neurons, axonal regeneration, and functional recovery. Our results show that most of the transplanted NSCs expressed glial fibrillary acidic protein, while there was no obvious expression of nestin, neuronal nuclei, or acetyltransferase found in NSCs. LiCl treatment produced less macrosialin (ED1) expression and axonal degeneration in tibial nerve after NSC injection. Our results also show that a regimen of LiCl treatment promoted NSC differentiation into NF200-positive neurons with neurite extension into the host spinal cord. The combination of tibial nerve transplantation with NSCs and LiCl injection resulted in more host motoneurons surviving in the spinal cord, more regenerated axons in tibial nerve, less glial scar area, and decreased ED1 expression. We conclude that lithium may have therapeutic potential in cell replacement strategies for central nervous system injury due to its ability to promote survival and neuronal generation of grafted NSCs and reduced host immune reaction.

show abstract

Section: Introductionmentioning

confidence: 99%

Transplantation of a Peripheral Nerve with Neural Stem Cells Plus Lithium Chloride Injection Promote the Recovery of Rat Spinal Cord Injury

Zhang

Deng

et al. 2018

Cell Transplant

View full text Add to dashboard Cite

show abstract

“…The risk of damaged brain. Indeed, stem cells have been demonstrated to differentiate into neurons (2,3,30). Transplanintrapartum neonatal asphyxia is estimated at 2.5% of all live births.…”

Section: Introductionmentioning

confidence: 99%

“…Overall, a phic lateral sclerosis (36), and other neurological disorders (2,3,20). These studies provide solid evidence of component of these patients with significant injury due to neonatal stroke or HI injury are easily identifiable therapeutic potential of transplanting stem cells in the clinic for patients with neurological disorders.…”

Section: Introductionmentioning

confidence: 99%

Behavioral and Histological Characterization of Intrahippocampal Grafts of Human Bone Marrow-Derived Multipotent Progenitor Cells in Neonatal Rats with Hypoxic-Ischemic Injury

Yasuhara

Matsukawa

et al. 2006

Cell Transplant

View full text Add to dashboard Cite

Children born with hypoxic-ischemic (HI) brain injury account for a significant number of live births wherein no clinical treatment is available. Limited clinical trials of stem cell therapy have been initiated in a number of neurological disorders, but the preclinical evidence of a cell-based therapy for neonatal HI injury remains in its infancy. One major postulated mechanism underlying therapeutic benefits of stem cell therapy involves stimulation of endogenous neurogenesis via transplantation of exogenous stem cells. To this end, transplantation has targeted neurogenic sites, such as the hippocampus, for brain protection and repair. The hippocampus has been shown to secrete growth factors, especially during the postnatal period, suggesting that this brain region presents as highly conducive microenvironment for cell survival. Based on its neurogenic and neurotrophic factor-secreting features, the hippocampus stands as an appealing target for stem cell therapy. Here, we investigated the efficacy of intrahippocampal transplantation of multipotent progenitor cells (MPCs), which are pluripotent progenitor cells with the ability to differentiate into a neuronal lineage. Seven-day-old Sprague-Dawley rats were initially subjected to unilateral HI injury, which involved permanent ligation of the right common carotid artery and subsequent exposure to hypoxic environment. At day 7 after HI injury, animals received stereotaxic hippocampal injections of vehicle or cryopreserved MPCs (thawed just prior to transplantation) derived either from Sprague-Dawley rats (syngeneic) or Fisher rats (allogeneic). All animals were treated with daily immunosuppression throughout the survival period. Behavioral tests were conducted on posttransplantation days 7 and 14 using the elevated body swing test and the rotarod to reveal general and coordinated motor functions. MPC transplanted animals exhibited reduced motor asymmetry and longer time spent on the rotarod than those that received the vehicle infusion. Both syngeneic and allogeneic MPC transplanted injured animals did not significantly differ in their behavioral improvements at both test periods. Immunohistochemical evaluations of graft survival after behavioral testing at day 14 posttransplantation revealed that syngeneic and allogeneic transplanted MPCs survived in the hippocampal region. These results demonstrate for the first time that transplantation of MPCs ameliorated motor deficits associated with HI injury. In view of comparable behavioral recovery produced by syngeneic and allogeneic MPC grafts, allogeneic transplantation poses as a feasible and efficacious cell replacement strategy with direct clinical application. An equally major finding is the observation lending support to the hippocampus as an excellent target brain region for stem cell therapy in treating HI injury.

show abstract

“…NSCs have been shown to differentiate into neurons and glia cells [22,23], and after grafting, a majority of NSCs differentiate into glia cells and a minority of NSCs differentiate into neurons in vivo [24]. NSCs originating from fetal rat hippocampus display a capacity of differentiating into Schwann cells that play a pivotal role in the process of peripheral nerve regeneration [25].…”

Section: Discussionmentioning

confidence: 99%

The Delayed Repair of Sciatic Nerve Defects with Tissue-engineered Nerve Grafts in Rats

Shi

Yao

Chen

et al. 2010

Artificial Cells, Blood Substitutes, and Biotechnology

View full text Add to dashboard Cite

The purpose of this study was to evaluate the feasibility of using tissue-engineered nerve grafts for delayed repair of peripheral nerve defects. A 1-month delayed, 10-mm long sciatic nerve defect was created for rats, which were divided into three grafted groups and a non-grafted group. For bridging the nerve defects, the rats in three grafted groups were subjected to surgical repair with tissue-engineered nerve grafts made of a chitosan/polyglycolic acid (PGA) conduit filled with neural stem cells (NSCs), chitosan/PGA conduits, and autologous nerve grafts, respectively. At 3 months after nerve grafting, the data from electrophysiology, retrograde tracing and histological investigation revealed that the better outcomes in sciatic nerve regeneration and target muscle re-innervation were achieved in three grafted groups as compared to those in non-grafted group without major differences between three grafted groups. Our results suggest that grafting of chitosan/PGA conduits might be a promising technique for repairing peripheral nerve injuries after a 1-month delay, while introduction of NSCs seem to show no significant additional benefits to regenerative outcomes.

show abstract

Embryonic cerebral cortex cells retain CNS phenotypes after transplantation into peripheral nerve

Cited by 10 publications

References 15 publications

Transplantation of a Peripheral Nerve with Neural Stem Cells Plus Lithium Chloride Injection Promote the Recovery of Rat Spinal Cord Injury

Transplantation of a Peripheral Nerve with Neural Stem Cells Plus Lithium Chloride Injection Promote the Recovery of Rat Spinal Cord Injury

Behavioral and Histological Characterization of Intrahippocampal Grafts of Human Bone Marrow-Derived Multipotent Progenitor Cells in Neonatal Rats with Hypoxic-Ischemic Injury

The Delayed Repair of Sciatic Nerve Defects with Tissue-engineered Nerve Grafts in Rats

Contact Info

Product

Resources

About