Embryonic Stem Cell–like Population in Dupuytren’s Disease

Koh, Sabrina P.; On, Nicholas; Brasch, Helen D.; Chibnall, Alice M.; Armstrong, J. R.; Davis, Paul F.; Tan, Swee T.; Itinteang, Tinte

doi:10.1097/gox.0000000000001064

Cited by 14 publications

(18 citation statements)

References 36 publications

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“…The ESC markers examined in this study were selected for their ability to confer and maintain stem cell properties. 17,18 The expression of these markers has been well documented in a number of conditions, including cancer, 20,21 Dupuytren's disease, 26 keloid disorder, 27 infantile hemangioma, 28 and VM. 14 These findings suggest the presence of a primitive population with capabilities of self-renewal and differentiation, driving these processes.…”

Section: Discussionmentioning

confidence: 99%

Expression of Embryonic Stem Cell Markers in Microcystic Lymphatic Malformation

Eady

Brasch

Jongh

et al. 2019

Lymphatic Research and Biology

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Aim: To investigate the expression of embryonic stem cell (ESC) markers in microcystic lymphatic malformation (mLM). Methods and Results: Cervicofacial mLM tissue samples from nine patients underwent 3,3¢-diaminobenzidine (DAB) immunohistochemical (IHC) staining for ESC markers octamer-binding protein 4 (OCT4), homeobox protein NANOG, sex determining region Y-box 2 (SOX2), Krupple-like factor (KLF4), and proto-oncogene c-MYC. Transcriptional activation of these ESC markers was investigated using real-time polymerase chain reaction (RT-qPCR) and colorimetric in situ hybridization (CISH) on four and five of these mLM tissue samples, respectively. Immunofluorescence (IF) IHC staining was performed on three of these mLM tissue samples to investigate localization of these ESC markers. DAB and IF IHC staining demonstrated the expression of OCT4, SOX2, NANOG, KLF4, and c-MYC on the endothelium of lesional vessels with abundant expression of c-MYC and SOX2, which was also present on the cells within the stroma, in all nine mLM tissue samples. RT-qPCR and CISH confirmed transcriptional activation of all these ESC markers investigated. Conclusions: These findings suggest the presence of a primitive population on the endothelium of lesional vessels and the surrounding stroma in mLM. The abundant expression of the progenitor-associated markers SOX2 and c-MYC suggests that the majority are of progenitor phenotype with a small number of ESC-like cells.

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Section: Discussionmentioning

confidence: 99%

Expression of Embryonic Stem Cell Markers in Microcystic Lymphatic Malformation

Eady

Brasch

Jongh

et al. 2019

Lymphatic Research and Biology

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“…We recently demonstrated the presence of a more primitive ESC-like cell population within the microvessels of the tissues surrounding the DD nodules and cords. 14 This progenitor cell population may sustain the ESC-like cell population with downstream MSCs that give rise to myofibroblasts in DD. RAS might play a role in the development of DD, given its involvement in other fibrotic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Hematoxylin and eosin staining was performed on 4-μm thick formalin-fixed paraffin-embedded sections of DD tissues from 6 patients to confirm the presence of DD cords and nodules, as recently reported. 14 3,3-Diaminobenzidine (DAB) and immunofluorescent (IF) IHC staining was performed on sections of all DD cords and nodules using the Leica Bond Rx auto-stainer (Leica, Nussloch, Germany) with primary antibodies: PRR (1:2000; cat# AB40790, Abcam, Cambridge, Mass. ), ACE (1:100; cat# 3C5, AbD Serotec, Oxford, United Kingdom), ATIIR1 (1:30; cat# AB9391, Abcam), and ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, Littleton, Colo.).…”

Section: Methodsmentioning

confidence: 99%

“… 12 , 13 We have recently demonstrated the presence of an embryonic stem cell (ESC)–like cell population, localized to the endothelium of the microvessels adjacent to DD nodules and cords. 14 This observation suggests the possibility of dysregulation of this stem cell population that ultimately sustains the proliferation of the myofibroblasts. The observation that dermofasciectomy, in which the overlying skin and surrounding tissue is also excised is associated with significantly lower recurrence rates compared with fasciectomy, 6 implicates the involvement of the overlying skin and surrounding adipose tissue in the disease process.…”

Section: Introductionmentioning

confidence: 99%

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Embryonic Stem Cell-Like Population in Dupuytren’s Disease Expresses Components of the Renin-Angiotensin System

Koh

Brasch

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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“…Tumor stem cells are proposed to be the cellular origin of cancer including glioblastoma (GB) ( 3 ) and leukemia ( 4 ), are increasingly thought to be the origin of benign entities such as Dupuytren's disease ( 5 ), infantile hemangioma ( 6 ) and MG ( 1 , 7 – 9 ). Cultured MG cells form tumor spheres ( 8 ), demonstrate self-renewal, and express embryonic stem cell (ESC) associated markers including SOX2, nestin ( 1 ) and KLF4 ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Expression of Embryonic Stem Cell Markers on the Microvessels of WHO Grade I Meningioma

et al. 2018

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Aim: The presence of cells within meningioma (MG) that express embryonic stem cell (ESC) markers has been previously reported. However, the precise location of these cells has yet to be determined.Methods: 3,3-Diaminobenzidine (DAB) immunohistochemical (IHC) staining was performed on 11 WHO grade I MG tissue samples for the expression of the ESC markers OCT4, NANOG, SOX2, KLF4 and c-MYC. Immunofluorescence (IF) IHC staining was performed to investigate the localization of each of these ESC markers. NanoString and colorimetric in situ hybridization (CISH) mRNA expression analyses were performed on six snap-frozen MG tissue samples to confirm transcriptional activation of these proteins, respectively.Results: DAB IHC staining demonstrated expression of OCT4, NANOG, SOX2, KLF4, and c-MYC within all 11 MG tissue samples. IF IHC staining demonstrated the expression of the ESC markers OCT4, NANOG, SOX2, KLF4, and c-MYC on both the endothelial and pericyte layers of the microvessels. NanoString and CISH mRNA analyses confirmed transcription activation of these ESC markers.Conclusion: This novel finding of the expression of all aforementioned ESC markers in WHO grade I MG infers the presence of a putative stem cells population which may give rise to MG.

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Embryonic Stem Cell–like Population in Dupuytren’s Disease

Abstract: Supplemental Digital Content is available in the text.

Cited by 14 publications

References 36 publications

Expression of Embryonic Stem Cell Markers in Microcystic Lymphatic Malformation

Expression of Embryonic Stem Cell Markers in Microcystic Lymphatic Malformation

Embryonic Stem Cell-Like Population in Dupuytren’s Disease Expresses Components of the Renin-Angiotensin System

Expression of Embryonic Stem Cell Markers on the Microvessels of WHO Grade I Meningioma

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