Embryonic Stem Cell Transplantation

Zhang, Feixiong; Pasumarthi, Kishore B.S.

doi:10.2165/0063030-200822060-00003

Cited by 38 publications

(5 citation statements)

References 144 publications

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“…ESCs are pluripotent cells with a capacity to differentiate to all cell types of the adult body and they can be cultured indefinitely in vitro through self-renewing division [ 30 – 32 ]. Therefore, ESCs are suitable for studying the initiation of mammalian organogenesis through differentiating ESCs into defined cell types [ 33 – 37 ].…”

Section: Introductionmentioning

confidence: 99%

STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

Wong

et al. 2018

Stem Cells International

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Studies have demonstrated that STAT3 is essential in maintaining self-renewal of embryonic stem cells (ESCs) and modulates ESC differentiation. However, there is still lack of direct evidence on STAT3 functions in ESCs and embryogenesis because constitutive STAT3 knockout (KO) mouse is embryonic lethal at E6.5–E7.5, prior to potential functional role in early development can be assessed. Therefore, in this study, two inducible STAT3 ESC lines were established, including the STAT3 knockout (InSTAT3 KO) and pSTAT3 overexpressed (InSTAT3 CA) using Tet-on inducible system in which STAT3 expression can be strictly controlled by doxycycline (Dox) stimulation. Through genotyping, deletion of STAT3 alleles was detected in InSTAT3 KO ESCs following 24 hours Dox stimulation. Western blot also showed that pSTAT3 and STAT3 protein levels were significantly reduced in InSTAT3 KO ESCs while dominantly elevated in InSTAT3 CA ECSs upon Dox stimulation. Likewise, it was found that STAT3-null ESCs would affect the differentiation of ESCs into mesoderm and cardiac lineage. Taken together, the findings of this study indicated that InSTAT3 KO and InSTAT3 CA ESCs could provide a new tool to clarify the direct targets of STAT3 and its role in ESC maintenance, which will facilitate the elaboration of the mechanisms whereby STAT3 maintains ESC pluripotency and regulates ESC differentiation during mammalian embryogenesis.

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Section: Introductionmentioning

confidence: 99%

STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

Wong

et al. 2018

Stem Cells International

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“…4 and 5, were biologically degraded after two months of implantation [10]. It is credible that cardiac tissue could be developed by pre-differentiating these cells into progenitors using the differentiation factors such as VEFG, EGF, IGF [11][12][13] or enriching the scaffolds with growth factors before seeding the cells [3,4,14]. Examination of implanted scaffolds with fetal cells has shown patterns of immune rejection, calcification and formation of allogenic structures.…”

Section: Discussionmentioning

confidence: 99%

“…5 D) shows the structures of unidentified allogenic origin, integrated and survived in the site of implantation. According to literature, implantation of allogenic fetal cells is controversial [3,4,14]. There are data showing that fetal cells cannot be detected by the host immune system and escape the immune rejection due to their unique immunological properties [4,15].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, results from multiple laboratories suggest that fetal cardiomyocytes can couple functionally with host myocytes, stimulate formation of new blood vessels, and improve myocardial function. The accumulating clinical and experimental evidence indicates that mesenchymal stem cells are promising cell types in the treatment of cardiac dysfunction [1][2][3][4][5]. They may trigger production of reparative growth factors, replace damaged cells and create an environment that favors endogenous cardiac repair [6].…”

Section: Introductionmentioning

confidence: 99%

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Fate of artificial tissue scaffolds implanted into the rabbit epimyocard

Antanavičiūtė¹,

Pauža²,

Rysevaitė³

et al. 2010

Biologija

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Fetal and bone marrow stem cells are supposed to be the key players in stem-cell-based therapies. Our aim was to evaluate the potency of two different types of stem cells and their spontaneous behavior in vivo after implantation into the epimyocard of healthy rabbit heart ventricles. For this purpose, rabbit allogenic fetal and autologous bone marrow stem cells were seeded on collagen scaffolds and subsequently implanted into the epimyocard of heart ventricles. Two months later these scaffolds were removed and a histological analysis was performed. The results have shown that different types of stem cells have generated two different types of structures in the sites of implantation. Autologous bone marrow cells in the collagen scaffold showed a chondrogenic differentiation pathway. Fetal cells were destroyed by the host immune system, although formation of allogenic structures in the epimyocard was observed, implying that cells of different sources in collagen scaffolds, after implantation in vivo, undergo differentiation.

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“…[23] Ferrocenyl derivatives were used as bioactive molecule which was established recently and several reports showed that a large number of ferrocene containing compounds have interesting cytotoxic and DNA cleaving activities. [24][25][26][27][28][29][30][31][32] So, a challenge to prepare a new Schiff base ligand from coupling of 2acetylferrocene with 1,3-diaminopropane was considered and explore its applications. Different physicochemical techniques were utilized for the characterization of the free Schiff base ligand and its complexes.…”

Section: Introductionmentioning

confidence: 99%

Theoretical studies of new Schiff base ligand derived from 1,3‐diaminopropane and 2‐acetyl ferrocene and studying some applications of its metal complexes

Sayed

Mahmoud

Omar

2019

Applied Organom Chemis

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A novel bidentate Schiff base ligand (L) and some d‐transition metal chelates (Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Cu (II), Zn (II) and Cd (II)) were synthesized and characterized using various physicochemical and spectroscopic techniques like elemental analysis, IR, mass, UV–visible and thermal analysis. The spectroscopic data suggested that the parent Schiff base ligand coordinated to the metal ions through both imine nitrogen atoms. The molecular and electronic structure of the free ligand was optimized theoretically, and the quantum chemical parameters were calculated. The molecular structure can be used to investigate the coordination sites and the total charge density around each atom. The free ligand and its complexes were screened for their antimicrobial activities for various pathogenic bacteria and fungi. The anticancer activities of the free ligand, Cr (III), Mn (II) and Fe (III) complexes were screened against MCF‐7 cell line and found that Mn (II) complex has the lowest IC50 (15.90 μg/ml). Molecular docking was used to predict the binding between the free ligand with receptor of mutant human androgen (ARccr) derived from androgen‐independent prostate cancer (1GS4), crystal structure of yeast‐specific serine/threonine protein phosphatase (ppz1) of Candida Albicans (5JPE) and crystal structure of renal tumor suppressor protein, folliculin (3 V42) and to identify the binding mode and the crucial functional groups interacting with the three proteins.

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Embryonic Stem Cell Transplantation

Cited by 38 publications

References 144 publications

STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

Fate of artificial tissue scaffolds implanted into the rabbit epimyocard

Theoretical studies of new Schiff base ligand derived from 1,3‐diaminopropane and 2‐acetyl ferrocene and studying some applications of its metal complexes

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