Embryotoxic effects of dissolved okadaic acid on the development of Longfin yellowtail Seriola rivoliana

Tovar‐Ramírez, Dariel; Núñez-Vázquez, Erick J.

doi:10.1016/j.aquatox.2017.07.012

Cited by 20 publications

(14 citation statements)

References 24 publications

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“…Several studies have revealed that OA exposure can delay embryonic development and increase embryo mortality of the fish O. latipes [18,28], the frog X. laevis [17], the longfin yellowtail Seriola rivoliana [29], and the chicken G. gallus [19]. More importantly, it has been shown that OA can cross the placental barrier, suggesting that OA may cause more harm to fetuses compared to adults since fetus is more vulnerable [20].…”

Section: Discussionmentioning

confidence: 99%

Okadaic Acid Exposure Induced Neural Tube Defects in Chicken (Gallus gallus) Embryos

Jiao

Wang

et al. 2021

Marine Drugs

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Okadaic acid (OA) is an important liposoluble shellfish toxin distributed worldwide, and is mainly responsible for diarrheic shellfish poisoning in human beings. It has a variety of toxicities, including cytotoxicity, embryonic toxicity, neurotoxicity, and even genotoxicity. However, there is no direct evidence of its developmental toxicity in human offspring. In this study, using the chicken (Gallus gallus) embryo as the animal model, we investigated the effects of OA exposure on neurogenesis and the incidence of neural tube defects (NTDs). We found that OA exposure could cause NTDs and inhibit the neuronal differentiation. Immunofluorescent staining of pHI3 and c-Caspase3 demonstrated that OA exposure could promote cell proliferation and inhibit cell apoptosis on the developing neural tube. Besides, the down-regulation of Nrf2 and increase in reactive oxygen species (ROS) content and superoxide dismutase (SOD) activity in the OA-exposed chicken embryos indicated that OA could result in oxidative stress in early chick embryos, which might enhance the risk of the subsequent NTDs. The inhibition of bone morphogenetic protein 4 (BMP4) and Sonic hedgehog (Shh) expression in the dorsal neural tube suggested that OA could also affect the formation of dorsolateral hinge points, which might ultimately hinder the closure of the neural tube. Transcriptome and qPCR analysis showed the expression of lipopolysaccharide-binding protein (LBP), transcription factor AP-1 (JUN), proto-oncogene protein c-fos (FOS), and C-C motif chemokine 4 (CCL4) in the Toll-like receptor signaling pathway was significantly increased in the OA-exposed embryos, suggesting that the NTDs induced by OA might be associated with the Toll-like receptor signaling pathway. Taken together, our findings could advance the understanding of the embryo–fetal developmental toxicity of OA on human gestation.

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Section: Discussionmentioning

confidence: 99%

Okadaic Acid Exposure Induced Neural Tube Defects in Chicken (Gallus gallus) Embryos

Jiao

Wang

et al. 2021

Marine Drugs

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“…L −1 ). Le Du and colleagues [29] were also able to show that OA and DTX-1 significantly inhibit protein and alkaline phosphatases, affecting the regulation pathways associated with embryogenesis and altering gene expression.…”

Section: Investigation Of Oa Effects In Marine Fish Under Laboratory Controlled Conditionsmentioning

confidence: 96%

“…In addition to its role as a strong inducer of diarrhetic activity, resulting from inhibition of proteins phosphatase (PP1 and PP2A), OA is known to exert cytotoxic, neurotoxic, immunotoxic, carcinogenic, and genotoxic effects [27]. Therefore, OA has been considered a key molecule with a high potential impact on the biology of aquatic animals, including effects on fitness and dependent functions of fishes [28,29]. J. Mar.…”

Section: Okadaic Acid-group Toxinsmentioning

confidence: 99%

“…Fish may be exposed to marine toxins, such as okadaic acid, in their early life sta Fish embryos may be particularly sensitive to marine toxins as they have not develo yet any effective enzymatic system to eliminate the toxins and may be adversely affe due to higher metabolic growth rates [33]. Therefore, a drastic impact on hatching suc was observed in fertilized eggs from natural spawning of longfin yellowtail (Seriola liana) exposed to two concentrations of dissolved OA and DTX-1 [29]. Reduction of in hatching success was observed in fish eggs exposed to the lowest concentration (12…”

Section: Investigation Of Oa Effects In Marine Fish Under Laboratory Controlled Conditionsmentioning

confidence: 99%

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Effects of the Marine Biotoxins Okadaic Acid and Dinophysistoxins on Fish

Corriere

Soliño

Costa

2021

JMSE

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Natural high proliferations of toxin-producing microorganisms in marine and freshwater environments result in dreadful consequences at the socioeconomically and environmental level due to water and seafood contamination. Monitoring programs and scientific evidence point to harmful algal blooms (HABs) increasing in frequency and intensity as a result of global climate alterations. Among marine toxins, the okadaic acid (OA) and the related dinophysistoxins (DTX) are the most frequently reported in EU waters, mainly in shellfish species. These toxins are responsible for human syndrome diarrhetic shellfish poisoning (DSP). Fish, like other marine species, are also exposed to HABs and their toxins. However, reduced attention has been given to exposure, accumulation, and effects on fish of DSP toxins, such as OA. The present review intends to summarize the current knowledge of the impact of DSP toxins and to identify the main issues needing further research. From data reviewed in this work, it is clear that exposure of fish to DSP toxins causes a range of negative effects, from behavioral and morphological alterations to death. However, there is still much to be investigated about the ecological and food safety risks related to contamination of fish with DSP toxins.

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“…A few exceptions are related with the study of OA-induced effects in fish. Concerning these aquatic vertebrates, DSTs (dissolved and crude extracts) are known to induce severe damage in embryonic and larval stages of several fish species, from sub-lethal (e.g., inhibition of both protein and alkaline phosphatases) to lethal effects [ 85 , 86 ]. The OA-group toxins are also known to negatively impact the behavior of some studied juvenile and adult fish species, causing oxidative stress, histological alterations and even death [ 87 , 88 , 89 ].…”

Section: Ecotoxicological Effects Of Dstsmentioning

confidence: 99%

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Campos

Freitas

Almeida

et al. 2020

Toxins

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Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

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Embryotoxic effects of dissolved okadaic acid on the development of Longfin yellowtail Seriola rivoliana

Cited by 20 publications

References 24 publications

Okadaic Acid Exposure Induced Neural Tube Defects in Chicken (Gallus gallus) Embryos

Okadaic Acid Exposure Induced Neural Tube Defects in Chicken (Gallus gallus) Embryos

Effects of the Marine Biotoxins Okadaic Acid and Dinophysistoxins on Fish

OMICs Approaches in Diarrhetic Shellfish Toxins Research

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