Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the β1 subunit of sodium–potassium ATPase in mouse embryos

Xu, Jianbing; Lee, Y.L.; Lee, K.F.; Kwok, Ka-Leung; Lee, W.M.; Luk, John M.; Yeung, William S.B.

doi:10.1093/humrep/deh497

Cited by 17 publications

(6 citation statements)

References 53 publications

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“…One explanation for the observed increase in apoptosis with co-culture may be that the rapid rate of blastomere proliferation combined with excellent ICM differentiation, triggered a compensatory increase in programmed cell death to retain appropriate balance between ICM and trophectodermal compartments. This may not have been observed with other co-culture systems such as primary human oviduct [28,29] and mouse uterine cells [50], because the mean cell number in co-cultured blastocysts was far lower (i.e. 48, 63 and 62, respectively).…”

Section: Discussionmentioning

confidence: 90%

“…The overall low levels of apoptosis reflect positively on the quality of embryos and the basal medium used in this study. Apoptotic indices of freshly isolated embryos in an un-supplemented control medium has ranged from (1.5 to 32%) in other published studies, depending on the animal species, culture medium and embryo stage [4,5,10,12,28,29,44,49].…”

Section: Discussionmentioning

confidence: 99%

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Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Desai

Kattal

AbdelHafez

et al. 2007

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Desai

Kattal

AbdelHafez

et al. 2007

J Assist Reprod Genet

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“…Interestingly, all three transcription factors are partially involved in proliferation processes. ETF is known as a critical determinant of proliferation 25. E2F transcription factors are well known as critical regulators of genes required for appropriate progression through the cell cycle 26, 27…”

Section: Discussionmentioning

confidence: 99%

Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytes

et al. 2010

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The cellular basis of liver regeneration has been intensely investigated for many years. However, the mechanisms initiating hepatocyte ''plasticity'' and priming for proliferation are not yet fully clear. We investigated alterations in gene expression patterns during the first 72 hours of C57BL/6N mouse hepatocyte culture on collagen monolayers (CM), which display a high basal frequency of proliferation in the absence of cytokines. Although many metabolic genes were down-regulated, genes related to mitogen-activated protein kinase (MAPK) signaling and cell cycle were up-regulated. The latter genes showed an overrepresentation of transcription factor binding sites (TFBS) for ETF (TEA domain family member 2), E2F1 (E2F transcription factor 1), and SP-1 (Sp1 transcription factor) (P < 0.001), all depending on MAPK signaling. Time-dependent increase of ERK1/2 phosphorylation occurred during the first 48 hours (and beyond) in the absence of cytokines, accompanied by an enhanced bromodeoxyuridine labeling index of 20%. The MEK inhibitor PD98059 blunted these effects indicating MAPK signaling as major trigger for this cytokine-independent proliferative response. In line with these in vitro findings, liver tissue of mice challenged with CCl 4 displayed hepatocytes with intense p-ERK1/2 staining and nuclear SP-1 and E2F1 expression. Furthermore, differentially expressed genes in mice after partial hepatectomy contained overrepresented TFBS for ETF, E2F1, and SP-1 and displayed increased expression of E2F1. Conclusion: Cultivation of murine hepatocytes on CM primes cells for proliferation through cytokine-independent activation of MAPK signaling. The transcription factors ETF, E2F1, and SP-1 seem to play a pronounced role in mediating proliferation-dependent differential gene expression. Similar events, but on a shorter time-scale, occur very early after liver damage in vivo. (HEPATOLOGY 2010;52:2127-2136 I n normal liver, hepatocytes remain in the quiescent G 0 phase. Loss of liver mass by partial hepatectomy (PHx) or hepatotoxic chemicals (e.g., CCl 4 ) induces a complex regeneration process restoring the original liver mass within 5-7 days. Within minutes after partial hepatectomy, signals occur that prime hepatocytes for proliferation. A broad range of cytokines are released and transcription factors are activated (summarized in recent reviews [1][2][3][4] ). In addition to cytokine signaling, the extracellular matrix (ECM) plays an Abbreviations: BrdU, 5-bromo-2 0

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“…Embryotrophic factor-3 from human oviductal cells plays a significant role in enhancing pre-implantation embryo development by promoting proliferation and inhibiting apoptosis (Xu et al 2004). Epidermal growth factor (EGF) (Adachi et al 1995), transforming growth factor (TGF) , insulin-like growth factor (IGF) (Carlsson et al 1993, Pfeifer & Chegini 1994, Daliri et al 1999 and fibroblast growth factor (FGF) are all detected in human Fallopian tissues (Fig.…”

Section: Growth Factorsmentioning

confidence: 99%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

208

191

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Animal oviducts and human Fallopian tubes are a part of the female reproductive tract that hosts fertilization and pre-implantation development of the embryo. With an increasing understanding of roles of the oviduct at the cellular and molecular levels, current research signifies the importance of the oviduct on naturally conceived fertilization and pre-implantation embryo development. This review highlights the physiological conditions within the oviduct during fertilization, environmental regulation, oviductal fluid composition and its role in protecting embryos and supplying nutrients. Finally, the review compares different aspects of naturally occurring fertilization and assisted reproductive technology (ART)-achieved fertilization and embryo development, giving insight into potential areas for improvement in this technology.

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Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the β1 subunit of sodium–potassium ATPase in mouse embryos

Abstract: ETF-3 improves embryo development by enhancing proliferation, suppressing apoptosis and stimulating expression of genes related to blastocyst cavitation. Supplementating human embryo culture medium with ETF-3 may improve the success rate in clinical assisted reproduction.

Cited by 17 publications

References 53 publications

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytes

Oviduct: roles in fertilization and early embryo development

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