Emerging cell cycle related non-coding RNA biomarkers from saliva and blood for oral squamous cell carcinoma

Kalmatte, Asrarunissa; Rekha, Punchappady Devasya; Ratnacaram, Chandrahas Koumar

doi:10.1007/s11033-023-08791-w

Cited by 3 publications

(3 citation statements)

References 104 publications

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“…MiRNAs, a class of crucial epigenetic regulators of gene expression, have emerged as key players in precision oncology, serving as ideal biomarkers for liquid biopsy, since they exhibit strong diagnostic potential by reflecting a tissue's malignant state through the quantification of their expressional dysregulation [3,8,9,12,16]. In recent years, the expression of numerous miRNAs has been studied in OSCC, resulting in a vast number of experimental data with at least 256 molecules reported to be either upregulated or downregulated in OSCC derived-samples (tissue, saliva or blood) [6,8,9,[17][18][19][20][21][22][23][24][25][26][27][28], while the majority exhibits similar dysregulation in other neoplasms as well. Consequently, there is a state of disarray regarding the interpretation of findings and the identification of the most representative molecules for the disease at large, but particularly for its early asymptomatic stages [8].…”

Section: Discussionmentioning

confidence: 99%

“…From those 10 molecules, only 3 are experimentally reported to exhibit significant upregulation in OSCC-derived tissue and/or saliva samples (Table 4). Those 3 ultimate results, serving as the most stage-specific miRNAs governing OSCC early invasion, include miR-155-5p (Figure 19), which is characteristically upregulated in both tissue and saliva samples [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], miR-423-3p (Figure 20), which scores characteristically high levels in OSCC tumor specimens, as well as miR-34a-5p (Figure 21) that has been reported as upregulated in saliva of OSCC patients according to a minimal number of studies.…”

Section: Figure 17mentioning

confidence: 99%

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Identification of Stage-Specific microRNAs That Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Genetics and Epigenetics

Gintoni,

Vassiliou,

Chrousos

et al. 2024

Preprint

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Oral squamous cell carcinoma (OSCC) is a highly prevalent and aggressive malignancy, with mortality rates that reach 60%, mainly due to an excessive diagnostic delay. MiRNAs, a class of crucial epigenetic gene-expression regulators, have emerged as potential diagnostic biomarkers, with >200 molecules reported to be dysregulated in OSCC. We had previously established an in silico methodology for the identification of the most disease-specific molecules by bridging genetics and epigenetics. Here, we identified the stage-specific miRNAs that govern the asymptomatic early stages of oral tumorigenesis by exploiting the reverse interplay between miRNA-levels and their target-genes’ expression. Incorporating gene expression data from our group’s experimental hamster model of sequential oral oncogenesis, we bioinformatically detected the miRNAs that simultaneously target/regulate >75% of the genes that are characteristically upregulated or downregulated in the consecutive stages of hyperplasia, dysplasia and early-invasion OSCC, while exhibiting the opposite expressional dysregulation in OSCC-derived tissue and/or saliva specimens. We found that all stages share the downregulation of miR-34a-5p, miR124-3p, and miR-125b-5p, while miR-1-3p is underexpressed in dysplasia and early-invasion. Malignant early-invasion stage is distinguished by the downregulation of miR-147a and the overexpression of miR-155-5p, miR-423-3p and miR-34a-5p. The identification of stage-specific miRNA molecules may facilitate their utilization as biomarkers for presymptomatic OSCC diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Figure 17mentioning

confidence: 99%

Identification of Stage-Specific microRNAs That Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Genetics and Epigenetics

Gintoni,

Vassiliou,

Chrousos

et al. 2024

Preprint

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“…Saliva is probably the most attractive secretion for the finding of biomarkers for OSCC and OPMDs, as its collection is easy, non-invasive, fast, and cost-effective. Thus, different studies have explored the possibility of using salivary factors as biomarkers for the development and progression of OSCC and OPMDs [ 9 , 10 , 11 ]. The aim of this study was to perform an exploratory systematic review about the different salivary molecules that have been assessed as possible biomarkers for the diagnosis of OSCC.…”

Section: Introductionmentioning

confidence: 99%

Salivary Biomarkers for Oral Cancer Detection: An Exploratory Systematic Review

Bastías,

Maturana,

Marín

et al. 2024

IJMS

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Different efforts have been made to find better and less invasive methods for the diagnosis and prediction of oral cancer, such as the study of saliva as a source of biomarkers. The aim of this study was to perform a scoping review about salivary molecules that have been assessed as possible biomarkers for the diagnosis of oral squamous cell carcinoma (OSCC). A search was conducted using EBSCO, PubMed (MEDLINE), Scopus, and Web of Science. The research question was as follows: which molecules present in saliva have utility to be used as biomarkers for the early detection of oral cancer? Sixty-two studies were included. Over 100 molecules were assessed. Most of the markers were oriented towards the early diagnosis of OSCC and were classified based on their ability for detecting OSCC and oral potentially malignant disorders (OPMDs), OSCC outcome prediction, and the prediction of the malignant transformation of OPMDs. TNF-α, IL-1β, IL-6 IL-8, LDH, and MMP-9 were the most studied, with almost all studies reporting high sensitivity and specificity values. TNF-α, IL-1β, IL-6 IL-8, LDH, and MMP-9 are the most promising salivary biomarkers. However, more studies with larger cohorts are needed before translating the use of these biomarkers to clinical settings.

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Identification of Stage-Specific microRNAs that Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Human Genetics with Epigenetics and Utilizing an Animal Model

Gintoni,

Vassiliou,

Chrousos

et al. 2024

IJMS

View full text Add to dashboard Cite

Oral squamous cell carcinoma (OSCC) is a highly prevalent and aggressive malignancy, with mortality rates reaching 60%, mainly due to its excessive diagnostic delay. MiRNAs, a class of crucial epigenetic gene-expression regulators, have emerged as potential diagnostic biomarkers, with >200 molecules exhibiting expressional dysregulation in OSCC. We had previously established an in silico methodology for the identification of the most disease-specific molecules by bridging genetics and epigenetics. Here, we identified the stage-specific miRNAs that govern the asymptomatic early stages of oral tumorigenesis by exploiting seed-matching and the reverse interplay between miRNA levels and their target genes’ expression. Incorporating gene-expression data from our group’s experimental hamster model of sequential oral oncogenesis, we bioinformatically detected the miRNAs that simultaneously target/regulate >75% of the genes that are characteristically upregulated or downregulated in the consecutive stages of hyperplasia, dysplasia, and early invasion, while exhibiting the opposite expressional dysregulation in OSCC-derived tissue and/or saliva specimens. We found that all stages share the downregulation of miR-34a-5p, miR124-3p, and miR-125b-5p, while miR-1-3p is under-expressed in dysplasia and early invasion. The malignant early-invasion stage is distinguished by the downregulation of miR-147a and the overexpression of miR-155-5p, miR-423-3p, and miR-34a-5p. The identification of stage-specific miRNAs may facilitate their utilization as biomarkers for presymptomatic OSCC diagnosis.

show abstract

Emerging cell cycle related non-coding RNA biomarkers from saliva and blood for oral squamous cell carcinoma

Cited by 3 publications

References 104 publications

Identification of Stage-Specific microRNAs That Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Genetics and Epigenetics

Identification of Stage-Specific microRNAs That Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Genetics and Epigenetics

Salivary Biomarkers for Oral Cancer Detection: An Exploratory Systematic Review

Identification of Stage-Specific microRNAs that Govern the Early Stages of Sequential Oral Oncogenesis by Strategically Bridging Human Genetics with Epigenetics and Utilizing an Animal Model

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