Emerging Ideas: Engineering the Periosteum: Revitalizing Allografts by Mimicking Autograft Healing

Hoffman, Michael D.; Benoit, Danielle S. W.

doi:10.1007/s11999-012-2695-7

Cited by 33 publications

(36 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to this example of in situ tissue engineering, investigations are on-going that aim to produce an engineered periosteum in vitro [101]. It is proposed that growth factors and cells can be sequestered within hydrogels in a manner and at levels that mimic the native periosteum.…”

Section: Tissue Engineering Strategies Mimicking the Periosteum In Itmentioning

confidence: 99%

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Roberts

Gastel

Carmeliet

et al. 2015

Bone

209

186

View full text Add to dashboard Cite

The cartilage- and bone-forming properties of the periosteum have long since been recognized. As one of the major sources of skeletal progenitor cells, the periosteum plays a crucial role not only in bone development and growth, but also during bone fracture healing. Aided by the continuous expansion of tools and techniques, we are now starting to acquire more insight into the specific role and regulation of periosteal cells. From a therapeutic point of view, the periosteum has attracted much attention as a cell source for bone tissue engineering purposes. This interest derives not only from the physiological role of the periosteum during bone repair, but is also supported by the unique properties and marked bone-forming potential of expanded periosteum-derived cells. We provide an overview of the current knowledge of periosteal cell biology, focusing on the cellular composition and molecular regulation of this remarkable tissue, as well as the application of periosteum-derived cells in regenerative medicine approaches. This article is part of a Special Issue entitled "Stem Cells and Bone".

show abstract

Section: Tissue Engineering Strategies Mimicking the Periosteum In Itmentioning

confidence: 99%

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Roberts

Gastel

Carmeliet

et al. 2015

Bone

209

186

View full text Add to dashboard Cite

show abstract

“…Considering the wide diversity of conducted research, investigators should be extremely cautious to translate conclusion in-between models and species and pay special attention in designing the animal studies to draw valuable and reproducible results. Mouse: [185], [186] Rat: [187] (DM), [188] Rabbit: [189], [190], [191], [192] Dog: [193], [194] Sheep/Goat: [167], [170], [176], [195], [196], [197] human BM-derived MPCs…”

Section: Animal Models In Cell-based Therapiesmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

130

116

View full text Add to dashboard Cite

“…40,41 In addition, recent work has demonstrated the ability of PEG hydrogels to provide spatiotemporal control of MSC delivery in vivo to promote bone regeneration. 31,42,43 In this work, methods have been explored to encapsulate, culture, and characterize primary SMG cells within PEG hydrogels, with the long-term goal of developing a tissue engineering approach for the salivary gland. Due to the sensitivity of salivary gland cells to reactive oxygen species (ROS), [44][45][46][47][48] we examined the effects of two forms of radical-mediated hydrogel polymerization: chain addition methacrylate-based polymerizations and step-growth thiol-ene polymerizations on primary SMG cells.…”

mentioning

confidence: 99%

“…Previous work using a PEG hydrogel periosteum mimetic to deliver MSCs significantly improved bone healing in a segmental defect model. 31,42 Critical to this approach was the localization and temporal release of MSCs provided by the PEG hydrogel, 43 as MSCs seeded directly on an allograft have little effect on healing. [107][108][109] In previous studies, transplantation of primary SMG cells into irradiated glands was performed using intraglandular *5-10 mL injections.…”

mentioning

confidence: 99%

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced xerostomia, but they suffer from insufficient and inconsistent gland regeneration and accompanying secretory function. To aid in the development of regenerative therapies, poly(ethylene glycol) hydrogels were investigated for the encapsulation of primary submandibular gland (SMG) cells for tissue engineering applications. Different methods of hydrogel formation and cell preparation were examined to identify cytocompatible encapsulation conditions for SMG cells. Cell viability was much higher after thiol-ene polymerizations compared with conventional methacrylate polymerizations due to reduced membrane peroxidation and intracellular reactive oxygen species formation. In addition, the formation of multicellular microspheres before encapsulation maximized cell-cell contacts and increased viability of SMG cells over 14-day culture periods. Thiol-ene hydrogel-encapsulated microspheres also promoted SMG proliferation. Lineage tracing was employed to determine the cellular composition of hydrogel-encapsulated microspheres using markers for acinar (Mist1) and duct (Keratin5) cells. Our findings indicate that both acinar and duct cell phenotypes are present throughout the 14 day culture period. However, the acinar:duct cell ratios are reduced over time, likely due to duct cell proliferation. Altogether, permissive encapsulation methods for primary SMG cells have been identified that promote cell viability, proliferation, and maintenance of differentiated salivary gland cell phenotypes, which allows for translation of this approach for salivary gland tissue engineering applications.

show abstract

Emerging Ideas: Engineering the Periosteum: Revitalizing Allografts by Mimicking Autograft Healing

Abstract: Background To fulfill the need for large volumes, devitalized allografts are used to treat massive bone defects despite a 60%, 10-year postimplantation fracture rate. Allograft healing is inferior to autografts where the periosteum orchestrates remodeling.

Cited by 33 publications

References 39 publications

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

The rational use of animal models in the evaluation of novel bone regenerative therapies

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Contact Info

Product

Resources

About