Emerging mass spectrometry techniques for the direct analysis of microbial colonies

Fang, Jinshu; Dorrestein, Pieter C.

doi:10.1016/j.mib.2014.06.014

Cited by 67 publications

(58 citation statements)

References 100 publications

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“…The hydrated agar assists in matrix-analyte crystallization during the drying process. This process is extremely robust as is evident by the number of researchers independently performing and achieving similar results [15]. However, this method is limited in the types of matrices that can be used for analysis (i.e., CHCA).…”

Section: Comparison Of Matrix Applicationsmentioning

confidence: 99%

Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures

Anderton

Chu

Tolić

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. The ability to visualize biochemical interactions between microbial communities using MALDI MSI has provided tremendous insights into a variety of biological fields. Matrix application using a sieve proved to be incredibly useful, but it has many limitations that include uneven matrix coverage and limitation in the types of matrices that could be employed in studies. Recently, there has been a concerted effort to improve matrix application for studying agar plated microbial cultures, many of which utilized automated matrix sprayers. Here, we describe the usefulness of using a robotic sprayer for matrix application. The robotic sprayer has twodimensional control over where matrix is applied, and a heated capillary that allows for rapid drying of the applied matrix. This method provided a significant increase in MALDI sensitivity over the sieve method, as demonstrated by FT-ICR MS analysis, facilitating the ability to gain higher lateral resolution MS images of Bacillus subtilis than previously reported. This method also allowed for the use of different matrices to be applied to the culture surfaces.

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Section: Comparison Of Matrix Applicationsmentioning

confidence: 99%

Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures

Anderton

Chu

Tolić

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…The newly developed imaging MS and real‐time MS allow two‐ and three‐dimensional visualization of the distribution of metabolites from microbial colonies and enable the identification of metabolites directly from microbial colonies (Yang et al ., 2009; Fang and Dorrestein, 2014). …”

Section: Novel Approaches For Drug Discoverymentioning

confidence: 99%

“…Among these new approaches, molecular networking (MN) is a particularly effective one to organize MS/MS fragmentation spectra. MN compares all MS/MS spectra in a given extract and groups them according to their similarity (Watrous et al ., 2012; Bandeira, 2011; Liu et al ., 2013; Fang and Dorrestein, 2014). The application of these tools leads to the identification of novel compounds by avoiding re‐isolation of known compounds.…”

Section: Dereplication In Natural Product Discoverymentioning

confidence: 99%

Antibiotic drug discovery

Wohlleben

Mast

Stegmann

et al. 2016

Microbial Biotechnology

143

113

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SummaryDue to the threat posed by the increase of highly resistant pathogenic bacteria, there is an urgent need for new antibiotics; all the more so since in the last 20 years, the approval for new antibacterial agents had decreased. The field of natural product discovery has undergone a tremendous development over the past few years. This has been the consequence of several new and revolutionizing drug discovery and development techniques, which is initiating a ‘New Age of Antibiotic Discovery’. In this review, we concentrate on the most significant discovery approaches during the last and present years and comment on the challenges facing the community in the coming years.

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“…The newly developed imaging MS and real-time MS allow two-and three-dimensional visualization of the distribution of metabolites from microbial colonies and enable the identification of metabolites directly from microbial colonies (Yang et al, 2009;Fang and Dorrestein, 2014).…”

Section: Alternative Methods For Drug Discoverymentioning

confidence: 99%

“…Among these new approaches, molecular networking (MN) is a particularly effective one to organize MS/MS fragmentation spectra. MN compares all MS/MS spectra in a given extract and groups them according to their similarity (Watrous et al, 2012;Bandeira, 2011;Liu et al, 2013;Fang and Dorrestein, 2014). The application of these tools leads to the identification of novel compounds by avoiding re-isolation of known compounds.…”

Section: Dereplication In Natural Product Discoverymentioning

confidence: 99%

Antibiotic Drug Discovery

Firestine¹,

Lister²

2017

Drug Discovery

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SummaryDue to the threat posed by the increase of highly resistant pathogenic bacteria, there is an urgent need for new antibiotics; all the more so since in the last 20 years, the approval for new antibacterial agents had decreased. The field of natural product discovery has undergone a tremendous development over the past few years. This has been the consequence of several new and revolutionizing drug discovery and development techniques, which is initiating a 'New Age of Antibiotic Discovery'. In this review, we concentrate on the most significant discovery approaches during the last and present years and comment on the challenges facing the community in the coming years.

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Emerging mass spectrometry techniques for the direct analysis of microbial colonies

Cited by 67 publications

References 100 publications

Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures

Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures

Antibiotic drug discovery

Antibiotic Drug Discovery

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