Emerging models: The “development” of the ctenophore Mnemiopsis leidyi and the cnidarian Nematostella vectensis as useful experimental models

Martindale, Mark Q.

doi:10.1016/bs.ctdb.2022.02.002

Cited by 3 publications

(2 citation statements)

References 95 publications

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“…Furthermore, its easy collection in the field, the establishment and maintenance of cell cultures, and its successful reproduction in vitro have turned M. leidyi into a model species for evolutionary and developmental studies (Martindale 2022). Hence, obtaining a chromosome-scale genome will also provide crucial genomic resources for many other studies, along with our analysis on the immune gene repertoire.…”

Section: Introductionmentioning

confidence: 99%

The chromosome-level genome of the ctenophoreMnemiopsis leidyiA. Agassiz, 1865 reveals a unique immune gene repertoire

Koutsouveli,

Torres-Oliva,

Bayer

et al. 2024

Preprint

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Ctenophora are basal marine metazoans, the sister group of all other animals. Mnemiopsis leidyi is one of the most successful invasive species worldwide with intense ecological and evolutionary research interest. Here, we generated a chromosome-level genome assembly of M. leidyi with a focus on its immune gene repertoire. The genome was 247.97 Mb, with N50 16.84 Mb, and 84.7% completeness. Its karyotype was 13 chromosomes. In this genome and that of two other ctenophores, Bolinopsis microptera and Hormiphora californensis, we detected a high number of protein domains related to potential immune receptors. Among those, proteins containing Toll/interleukin-1(TIR2) domain, NACHT domain, Scavenger Receptor Cystein-Rich (SRCR) domain, or C-type Lectin domain (CTLD) were abundant and presented unique domain architectures in M. leidyi. M. leidyi seems to lack bona fide Toll like Receptors, but it does possess a repertoire of 15 TIR2-domain containing genes. Besides, we detected a bona fide NOD-like receptor and 38 NACHT-domain containing genes. In order to verify the function of those domain containing genes, we exposed M. leidyi to the pathogen Vibrio coralliilyticus. Among the differentially expressed genes, we identified potential immune receptors, including four TIR2-domain containing genes, all of which were upregulated in response to pathogen exposure. To conclude, many common immune receptor domains, highly conserved across metazoans, are already present in Ctenophora. These domains have large expansions and unique architectures in M. leidyi, findings consistent with the basal evolutionary position of this group, but still might have conserved functions in immunity and host-microbe interaction.

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Section: Introductionmentioning

confidence: 99%

The chromosome-level genome of the ctenophoreMnemiopsis leidyiA. Agassiz, 1865 reveals a unique immune gene repertoire

Koutsouveli,

Torres-Oliva,

Bayer

et al. 2024

Preprint

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“…Due to the high ecological plasticity of Mnemiopsis leidyi , it has become an important invasive species that colonized a wide range of marine environments and disrupted native ecosystems (Burton, 2008). Such a plasticity along with high regenerative capacity (Edgar et al, 2021) probably explains why M. leidyi has become a lab model organism (Martindale, 2022) that can adapt to the artificial conditions of aquarium facilities and be successfully cultured and reproduced over multiple generations (Presnell et al, 2022; Soto‐Àngel et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary origin of the nervous system from Ctenophora prospective

Sachkova

2024

Evolution and Development

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Nervous system is one of the key adaptations underlying the evolutionary success of the majority of animal groups. Ctenophores (or comb jellies) are gelatinous marine invertebrates that were probably the first lineage to diverge from the rest of animals. Due to the key phylogenetic position and multiple unique adaptations, the noncentralized nervous system of comb jellies has been in the center of the debate around the origin of the nervous system in the animal kingdom and whether it happened only once or twice. Here, we discuss the latest findings in ctenophore neuroscience and multiple challenges on the way to build a clear evolutionary picture of the origin of the nervous system.

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Analysis and Visualization of Single-Cell Sequencing Data with Scanpy and MetaCell: A Tutorial

Li,

Sun,

Romanova

et al. 2024

Methods in Molecular Biology

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Emerging models: The “development” of the ctenophore Mnemiopsis leidyi and the cnidarian Nematostella vectensis as useful experimental models

Cited by 3 publications

References 95 publications

The chromosome-level genome of the ctenophoreMnemiopsis leidyiA. Agassiz, 1865 reveals a unique immune gene repertoire

The chromosome-level genome of the ctenophoreMnemiopsis leidyiA. Agassiz, 1865 reveals a unique immune gene repertoire

Evolutionary origin of the nervous system from Ctenophora prospective

Analysis and Visualization of Single-Cell Sequencing Data with Scanpy and MetaCell: A Tutorial

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