Emerging or Underestimated Silica-Based Stationary Phases in Liquid Chromatography

Sobańska, Anna W.

doi:10.1080/10408347.2020.1760782

Cited by 7 publications

(5 citation statements)

References 185 publications

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“…There are many different stationary phases, allowing separations to be tailored depending on the particular characteristics of the analytes in question. A detailed analysis of stationary phases is beyond the scope of this article, and there are already many excellent reviews on the subject (Zou et al, 2002;Zhang, 2008;West et al, 2010;Sobańska, 2021), but the most commonly applicable phases in metabolomics in general are reversed phase (RP, which separates on the basis of hydrophobicity) and hydrophilic interaction liquid chromatography (HILIC, which separates on the basis of hydrophilicity).…”

Section: Liquid Chromatographymentioning

confidence: 99%

Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics

Moses

Burgess

2023

Front. Mol. Biosci.

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This mini review focuses on the opportunities provided by current and emerging separation techniques for mass spectrometry metabolomics. The purpose of separation technologies in metabolomics is primarily to reduce complexity of the heterogeneous systems studied, and to provide concentration enrichment by increasing sensitivity towards the quantification of low abundance metabolites. For this reason, a wide variety of separation systems, from column chemistries to solvent compositions and multidimensional separations, have been applied in the field. Multidimensional separations are a common method in both proteomics applications and gas chromatography mass spectrometry, allowing orthogonal separations to further reduce analytical complexity and expand peak capacity. These applications contribute to exponential increases in run times concomitant with first dimension fractionation followed by second dimension separations. Multidimensional liquid chromatography to increase peak capacity in metabolomics, when compared to the potential of running additional samples or replicates and increasing statistical confidence, mean that uptake of these methods has been minimal. In contrast, in the last 15 years there have been significant advances in the resolution and sensitivity of ion mobility spectrometry, to the point where high-resolution separation of analytes based on their collision cross section approaches chromatographic separation, with minimal loss in sensitivity. Additionally, ion mobility separations can be performed on a chromatographic timescale with little reduction in instrument duty cycle. In this review, we compare ion mobility separation to liquid chromatographic separation, highlight the history of the use of ion mobility separations in metabolomics, outline the current state-of-the-art in the field, and discuss the future outlook of the technology. “Where there is one, you’re bound to divide it. Right in two”, James Maynard Keenan.

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Section: Liquid Chromatographymentioning

confidence: 99%

Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics

Moses

Burgess

2023

Front. Mol. Biosci.

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Section: Facing the Challenges In The Isolation Of Hydrophilic Compoundsmentioning

confidence: 99%

“…1). It has been recently stated that the use of such stationary phases has been largely underestimated for liquid chromatography separations, 244 even though reports on properties and versatile applications of amino-, cyano-and diolbonded Si-gel stationary phases were available for decades. [245][246][247][248][249] In our 1996-2009 survey of 4859 articles reporting the isolation of natural products, 34 articles (0.7%) reported the use of an aminopropyl-derivatized Si-gel column in at least one of the separation steps.…”

Section: Ion-exchange Chromatographymentioning

confidence: 99%

The isolation of water-soluble natural products – challenges, strategies and perspectives

et al. 2022

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“…As a result, a great deal of work has been carried out on the enantioseparation of racemic drugs by applying different varieties of CSPs, which are grouped into eight catagories, as shown in Table 3 . Overviews of different separation principles and various applications of CSPs are given in [ 21 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Enantioseparation Of β-Blockers By Hplcmentioning

confidence: 99%

Progress in the Enantioseparation of β-Blockers by Chromatographic Methods

et al. 2021

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β-adrenergic antagonists (β-blockers) with at least one chiral center are an exceedingly important class of drugs used mostly to treat cardiovascular diseases. At least 70 β-blockers have been investigated in history. However, only a few β-blockers, e.g., timolol, are clinically marketed as an optically pure enantiomer. Therefore, the separation of racemates of β-blockers is essential both in the laboratory and industry. Many approaches have been explored to obtain the single enantiomeric β-blocker, including high performance liquid chromatography, supercritical fluid chromatography and simulated moving bed chromatography. In this article, a review is presented on different chromatographic methods applied for the enantioseparation of β-blockers, covering high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC) and simulated moving bed chromatography (SMB).

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Emerging or Underestimated Silica-Based Stationary Phases in Liquid Chromatography

Cited by 7 publications

References 185 publications

Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics

Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics

The isolation of water-soluble natural products – challenges, strategies and perspectives

Progress in the Enantioseparation of β-Blockers by Chromatographic Methods

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