The Myanmar region experienced the subduction of the Indian Ocean plate to the West Burma block and suffered from the land–land collision between the Indian continent and the West Burma block that occurred from the Late Cretaceous to the Cenozoic. Its tectonic evolution has been complex; thus, oil and gas exploration is difficult, and the overall degree of research has been low. Recent exploration has been hindered by a lack of knowledge on the evolution of the petroleum system. To address this, we conducted hydrocarbon generation and accumulation modeling using both the 2D MOVE and Petro-Mod software 2017 for a complex tectonic section in the Northern Central Myanmar Basin. The results show that the maturity threshold depth of the Cretaceous source rocks in the study area is shallow, and the underground depth of 1200 m to 1400 m has reached the hydrocarbon generation threshold, indicating the start of hydrocarbon generation. Since 48 Ma, the Ro of the source rocks has reached 0.7%, became mature quite early. The Late Cretaceous Paleocene and Eocene formation, located in the southeastern part of the study area, migrated and accumulated hydrocarbons towards the western arc zone in the Eocene and Miocene, respectively. It is worth noting that although the oil and gas potential of each layer in the island arc uplift zone is relatively low, which is conducive to the migration and accumulation of oil and gas generated by the source rocks of the depression towards the island arc zone, shallow areas with developed extensional faults should be avoided. This study is the first to conduct a preliminary assessment and prediction of oil and gas resources, which will provide exploration guidance and reference for the study area and its surrounding areas in the future.