Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Xia, Yingcun; Li, Mengying; Chen, Chenglong; Meng, Xiangjian; Zhang, Wen; Gao, Wei‐Wei

doi:10.1002/elan.202060039

Cited by 29 publications

(10 citation statements)

References 61 publications

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“…Similarly, another DPV biosensor was reported to detect BRCA1. Xia et al [ 72 ] modified 3D reduced graphene oxide and polyaniline nanocomposites (3D-rGO-PANI) on the surface of a glassy carbon electrode as a sensing layer to improve the conductivity and electrochemical activity of the biosensor. After the target DNA BRCA1 was captured by the DNA probe that was modified on the surface of the 3D-rGO-PANI, the methylene blue would be used to further amplify the DPV signal of the biosensor.…”

Section: Biosensormentioning

confidence: 99%

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

Hong

Sun

et al. 2022

Life

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Breast cancer has the highest cancer incidence rate in women. Early screening of breast cancer can effectively improve the treatment effect of patients. However, the main diagnostic techniques available for the detection of breast cancer require the corresponding equipment, professional practitioners, and expert analysis, and the detection cost is high. Tumor markers are a kind of active substance that can indicate the existence and growth of the tumor. The detection of tumor markers can effectively assist the diagnosis and treatment of breast cancer. The conventional detection methods of tumor markers have some shortcomings, such as insufficient sensitivity, expensive equipment, and complicated operations. Compared with these methods, biosensors have the advantages of high sensitivity, simple operation, low equipment cost, and can quantitatively detect all kinds of tumor markers. This review summarizes the biosensors (2013–2021) for the detection of breast cancer biomarkers. Firstly, the various reported tumor markers of breast cancer are introduced. Then, the development of biosensors designed for the sensitive, stable, and selective recognition of breast cancer biomarkers was systematically discussed, with special attention to the main clinical biomarkers, such as human epidermal growth factor receptor-2 (HER2) and estrogen receptor (ER). Finally, the opportunities and challenges of developing efficient biosensors in breast cancer diagnosis and treatment are discussed.

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Section: Biosensormentioning

confidence: 99%

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

Hong

Sun

et al. 2022

Life

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“…Table 2 summarizes the analytical performance of the most relevant BRCA1 electrochemical biosensors reported in the last years. As can be seen, GCE/MWCNT-IgG/DNAp presents a very competitive analytical performance, with a lower detection limit [29][30][31][32][33][34][35] or comparable [36,37] than those shown in the table. Wang et al [29] proposed a differential pulse voltammetric (DPV) biosensor based on a nanocomposite of the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a novel antifouling polypeptide (PEP) electrodeposited on the surface of a GCE.…”

Section: Construction Of Brca1 Gene Biosensor and Analytical Applicat...mentioning

confidence: 79%

“…In this sense, the electrochemical biosensors represent an interesting analytical tool for competitive BRCA1 quantification. Several biosensors based on the use of polymers, modified carbon nanomaterials and/or antimonene, with or without amplification schemes, have been reported in the last years [29][30][31][32][33][34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Sensing Using Glassy Carbon Electrodes Modified with Multiwalled-Carbon Nanotubes Non-Covalently Functionalized with Human Immunoglobulin G

Mujica,

Tamborelli,

Dalmasso

et al. 2023

Chemosensors

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This work reports new analytical applications of glassy carbon electrodes (GCE) modified with a nanohybrid obtained by non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with human immunoglobulin G (IgG) (GCE/MWCNT-IgG). We report the label-free and non-amplified breast cancer 1 gen (BRCA1) biosensing based on the facilitated adsorption of the DNA probe at the nanohybrid modified GCE and the impedimetric detection of the hybridization event in the presence of the redox marker benzoquinone/hydroquinone. The resulting genosensor made the fast, highly selective, and sensitive quantification of BRCA1 gene possible, with a linear range between 1.0 fM and 10.0 nM, a sensitivity of (3.0 ± 0.1) × 102 Ω M−1 (R2 = 0.9990), a detection limit of 0.3 fM, and excellent discrimination of fully non-complementary and mismatch DNA sequences. The detection of BRCA1 in enriched samples of diluted human blood serum showed a recovery percentage of 94.6%. Another interesting analytical application of MWCNT-IgG-modified GCE based on the catalytic activity of the exfoliated MWCNTs is also reported for the simultaneous quantification of dopamine and uric acid in the presence of ascorbic acid, with detection limits at submicromolar levels for both compounds.

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“…Thus, it exhibits superior bioelectrochemical performance and supercapacitor properties . In general, 3D graphene can be obtained via 3D graphene oxide (3D-GO) reduction or with the use of gelation technologies with 2D-rGO sheets. , However, electrochemical deposition synthesis can be used as a novel method to obtain 3D graphene on the electrode surface . Moreover, the functionalization of 3D graphene can be performed simply with various metal oxides or polymers .…”

Section: Electrospun Nanofibers (Esnfs)mentioning

confidence: 99%

Carbon-Based Nanomaterials Decorated Electrospun Nanofibers in Biosensors: A Review

Kilic,

Gelen,

Er Zeybekler

et al. 2023

ACS Omega

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Nanomaterials have revolutionized scientific research due to their exceptional physical and chemical capabilities. Carbon-based nanomaterials such as graphene and its derivates have excellent electrical, optical, thermal, physical, and chemical properties that have made them indispensable in several industries worldwide, including medicine, electronics, and energy. By incorporating carbon-based nanomaterials as nanofillers in electrospun nanofibers (ESNFs), smoother and highly conductive nanofibers can be achieved that possess a large surface area and porosity. This approach provides a superior alternative to traditional materials in the development of improved biosensors. Carbon-based ESNFs, among the most exciting new-generation materials, have many applications, including filtration, pharmaceuticals, biosensors, and membranes. The electrospinning technique is a highly efficient and cost-effective method for producing desired nanofibers compared to other methods. Various types of natural and synthetic organic polymers have been successfully utilized in solution electrospinning to produce nanofibers directly. To create diagnostics devices, various biomolecules like antibodies, enzymes, aptamers, ligands, and even cells can be bound to the surface of nanofibers. Electrospun nanofibers can serve as an immobilization matrix to create a biofunctional surface. Thus, biosensors with desired features can be produced in this way. This study comprehensively reviews biosensors that integrate nanodiamonds, fullerenes, carbon nanotubes, graphene oxide, and carbon dots into electrospun nanofibers.

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Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Cited by 29 publications

References 61 publications

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

Label-Free Electrochemical Sensing Using Glassy Carbon Electrodes Modified with Multiwalled-Carbon Nanotubes Non-Covalently Functionalized with Human Immunoglobulin G

Carbon-Based Nanomaterials Decorated Electrospun Nanofibers in Biosensors: A Review

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