EMS-Based In Vitro Mutagenesis and Mutant Screening for Smut Resistance with Agronomic Traits in Sugarcane

Dalvi, Sunil G.; Tawar, P. N.; Suprasanna, Penna; Dixit, G. B.; Prasad, D.

doi:10.1007/s12355-020-00931-x

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…Srivastava et al, [21] identified RAPD marker linked to powdery mildew resistant gene(s) in black gram. The mutants in present study has been evaluated for smut resistance in filed for two years artificial inoculation trial and TC 906 and TC 2826 were reported to be resistant for smut [1,2]. Thus the distinct molecular fingerprinting patterns with these mutants open up new ways providing stable genetic differences for analyzing the resistance and susceptibility of sugarcane genotypes with the RAPD technique.…”

Section: Primer Selectionmentioning

confidence: 83%

“…Due to the merits of CoC 671 in-vitro mutagenesis and somaclonal variation was attempted by various researchers to rectify the defective agronomic traits in it [1][2][3][4][5][6][7] and two varieties as CoM 94012 and VSI 434 have been released by proving CoC 671 by somaclonal variation but none was having smut resistance in them. Dalvi et al [1,2] in the field following standard agronomic practices. The leaf samples for extraction of DNA were collected during the harvest at the 12 th month period.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis

G¹

2021

annagriccropsci

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Sugarcane is an important cash crop gaining importance as ideal raw material for bioenergy production. Conventional sugarcane improvement is carried out through intensive crossing and selection breeding within the Saccharum complex. Being highly polyploidy crop the mutation breeding is also integrated for specifically improving the desired genetic trait in sugarcane varieties developed through conventional breeding. In the present work RAPD markers based assessment of genetic diversity and kinship relationships of six promising mutants developed using CoC 671 has been reported. The banding pattern of the PCR amplified products showed polymorphism with the RAPD profile in all six mutants. Mutants CoC 671, TC 2813, TC 2819, TC 2826, TC 2875 formed a separate cluster, and TC 906, TC 922, and TC 906 B formed a separate cluster distinct from the earlier one with the parent (CoC 671) and the standard Check (Co 86023). The genetic distance between the groups was found to be only marginally higher than the respective group distances and the overall mean genetic distance. The similarity indices indicated that there was 15 % genetic dissimilarity between CoC 671 and Co 86032. This may be because CoC 671 was one of the parents for Co 86032. In the present investigation, the genetic similarity value ranged from 0.97 to 0.78 among the mutants. A total of 34 band positions were scored, out of which 15 RAPD bands were polymorphic. From the genetic similarity coefficient based on RAPD band data sharing, it was found that the majority of the clones were almost more than 78% similar to the mother plant. Thus the level of similarity between varieties seemed to be not very high but the mutants could be distinguished from each other easily. RAPD analysis indicated EMS-induced point mutations resulting in specific rectifications without much change in the genetic backbone of genotype CoC 671. Since this analysis was carried out on mutants after multiplying in field for three successive generations, we presume that these mutants/genotypes are stable and express the stable genetic variations.

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Section: Primer Selectionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis

G¹

2021

annagriccropsci

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“…Recently, this mutagen has achieved satisfactory results in the breeding of Fusarium-head-blight-resistant wheat plant (Chhabra et al 2021) and smut-resistant sugarcane strains (Dalvi et al 2021). 0.175% EMS also induced blast-resistant clones in rice varieties (Rakshit 2010).…”

Section: Effects Of Ems and Mesa Treatments On Blast Resistance In Th...mentioning

confidence: 99%

The effect of chemical-induced mutagenesis in indica rice variety IR50404 for blast disease caused by the Magnaporthe grisea in Vietnam

Tran,

Ha,

Henry

et al. 2024

IJGPB

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Indica rice variety, IR50404 is the most-grown rice in Vietnam’s Mekong Delta. It is susceptible to multiple blast diseases, making it an ideal genotype for identifying pathological trait mutations. Chemically induced mutant lines were derived from IR50404 by treating seeds with ethyl methanesulfonate (EMS) (0.1 and 0.125%), methyl salicylate (MeSA) (0.1 and 0.125 mM), and their combinations. Chlorophyll, proline, and protease enzyme inhibitors were examined for disease tolerance in mutant rice-based plants. Blast resistance (41.6%) and protease inhibition (30.4%) were highest in T5 (0.1% EMS + 0.1 mM MeSA). Proline (3.91 g/mL) and chlorophyll (8.52 g/mL) concentrations were significantly higher after 0.125% EMS treatment than in the untreated control.In addition, a high rate of protease inhibition (30.4%) was improved after treatment with 0.125% EMS + 0.125 mM MeSA. Results showed that blast resistance was improved by 0.1% EMS and MeSA.This also has implications for the breeding of plant varieties other than rice

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“…Mutants TC 2819 and TC 2826 exhibited superior for sucrose content (20-24%) than parent CoC 671 (18-21%), respectively, at 10 th and 12 th month of maturity. Their study concluded that EMSinduced genetic variability for the mutants with smut resistance and agronomic traits in sugarcane [120].…”

Section: Chemical Mutagenesismentioning

confidence: 99%

In-vitro mutagenesis Approaches for Flowering Control in Sugarcane – A Review

Nagesha

Kumar

Venkatesha

et al. 2022

IJECC

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Sugarcane (Saccharum officinarum L.) is one of the important commercial crops grown globally as a source of raw material for wide range of sugar and bio-energy industries. Crop being a member of family Poaceae, terminates its growth by flowering (arrowing). The process of flowering is highly complex process and is mediated by temperature, photoperiod, humidity, altitude and latitude besides crop genetic makeup. Though flowering is crucial for crop improvement programmes, uncontrolled flowering under commercial cultivation poses a serious problem for sugarcane farmers and millers with a considerable loss in yield and quality. Therefore, in order to device sugarcane genotype with no flowering habit enormous efforts have been made through various approaches, among them mutagenesis is one such approach wherein propagation materials will be exposed to chemicals or radiation in order to bring desirable traits. This technique looks to be the prominent crop improvement strategy for sugarcane. Hence, in this paper review we brought together the earlier work done in the field of mutation breeding in devising non-flowering sugarcane genotypes and their impacts on commercial sugarcane production.

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EMS-Based In Vitro Mutagenesis and Mutant Screening for Smut Resistance with Agronomic Traits in Sugarcane

Cited by 17 publications

References 33 publications

Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis

Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis

The effect of chemical-induced mutagenesis in indica rice variety IR50404 for blast disease caused by the Magnaporthe grisea in Vietnam

In-vitro mutagenesis Approaches for Flowering Control in Sugarcane – A Review

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