2022
DOI: 10.1016/j.biomaterials.2022.121669
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Emulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat

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Cited by 46 publications
(21 citation statements)
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“…[ 38,42 ] Our results suggest that Ca 2+ release from bioglass nanoparticles can be used to provide mechanical cues for mechanosensitive cells, such as myoblasts, to promote their differentiation into myotubes. [ 43–46 ]…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…[ 38,42 ] Our results suggest that Ca 2+ release from bioglass nanoparticles can be used to provide mechanical cues for mechanosensitive cells, such as myoblasts, to promote their differentiation into myotubes. [ 43–46 ]…”
Section: Resultsmentioning
confidence: 99%
“…[38,42] Our results suggest that Ca 2+ release from bioglass nanoparticles can be used to provide mechanical cues for mechanosensitive cells, such as myoblasts, to promote their differentiation into myotubes. [43][44][45][46] Of note, we also conducted experiments analogous to those with added bioglass but instead using silica nanoparticles at the same concentrations. The silica nanoparticles have a similar size and morphology to those of the bioglass particles that we used in our experiments.…”
Section: Production Of Axial Gradientsmentioning
confidence: 99%
“…Indeed, conjugating cell adhesion motifs to the polymer backbone is not always required, especially when the scaffold is made from animal-derived materials, such as collagen and gelatin. In some cases, nonmodified alginate scaffolds have supported cell adhesion in short-term culture conditions [ 30 , 31 , 32 ]; however, cell adhesion on nonmodified alginate was significantly lower compared to gelatin [ 32 ].…”
Section: Resultsmentioning
confidence: 99%
“…Substrate stiffness of approximately 8-16 kPa has been found optimal for myogenesis in certain cell types (Engler et al, 2004). Microcarriers should have a diameter of at least 100 lm, and larger microcarriers promote better cell attachment, while smaller ones result in higher growth rates due to increased shear stress (Norris et al, 2022). The review article by Bomkamp et al extensively examines the estimated requirements for suitable scaffolds in CM production.…”
Section: Requirements Of Scaffolds and Microcarriersmentioning
confidence: 99%
“…4b; they produced gelatin fibres with a diameter similar to the collagen fibres' diameter (MacQueen et al, 2019). Norris et al (2022) developed microcarriers by utilising waterin-oil emulsions as templates for gelatin microparticles by applying an embossing technique to create a grooved surface texture on the microcarriers, which showed that both smooth and grooved edible microcarriers effectively facilitated the growth and differentiation of C2C12 in suspension culture. Additionally, Liu et al employed a 3D porous gelatin microcarrier in two spinner flask bioreactors, one for muscle and the other for fat.…”
Section: Animal-based Protein Materialsmentioning
confidence: 99%