Enabling Single-Molecule Detection in Living Cells: Ultra-Sensitive Microscopy and Spectroscopy in 3D

Abstract

We demonstrate a cryogenic super-resolution correlative light and electron microscopy of vitrified specimens which were prepared by high pressure freezing and cryo-sectioning to maintain a close-to-native state with better fluorescence preservation. Several fluorescent proteins were found photoswitchable and emitted much more photons under cryo-condition, hence resulting in higher localization precision. We observed nice correlation of a mitochondria protein with mitochondria outer membrane at nanometer resolution in three dimensional.