Enalapril reduces collagen type IV synthesis and expansion of the interstitium in the obstructed rat kidney

Kaneto, Hiroyuki; Morrissey, Jeremiah J.; McCracken, Ruth; Reyes, A.; Klahr, Saulo

doi:10.1038/ki.1994.215

Cited by 203 publications

(146 citation statements)

References 30 publications

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…Renal tissue was obtained from shamtreated and obstructed kidneys with and without TNF-␣ neutralization (3 different samples per time point). Total RNA was extracted from the tissue by homogenization in Trizol (GIBCO BRL, Gaithersburg, MD) as previously described (19). PCR was performed by adding 2 l of RT product to a commercial dual-PCR supermix (Maxim Biotech, San Francisco, CA) containing Taq DNA polymerase and primers for both TGF-␤1 and GAPDH.…”

Section: Methodsmentioning

confidence: 99%

TNF-α neutralization ameliorates obstruction-induced renal fibrosis and dysfunction

Meldrum¹,

Misseri²,

Metcalfe³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

TNF-α neutralization ameliorates obstruction-induced renal fibrosis and dysfunction

Meldrum¹,

Misseri²,

Metcalfe³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Interstitial ␣-SMA expression. Interstitial immunostaining for ␣-SMA was quantified by the pointing counting method (22) using an eyepiece graticule of 1 cm 2 with 10 equidistant lines at a final magnification of ϫ400. The results were expressed as the percentage of ␣-SMA-positive area in the cortical interstitium calculated according to the following formula and averaged among more than 20 fields in each section.…”

Section: Morphometric Analysismentioning

confidence: 99%

Genetically Modified Bone Marrow-Derived Vehicle Cells Site Specifically Deliver an Anti-Inflammatory Cytokine to Inflamed Interstitium of Obstructive Nephropathy

Yamagishi

Yokoo

Imasawa

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

In this study, we used genetically modified bone marrow-derived CD11b+CD18+ vehicle cells to deliver IL-1 receptor antagonist (IL-1ra) for treatment of inflamed renal interstitium in an animal model of unilateral ureteral obstruction (UUO). Vehicle cells that expressed the ICAM-1 ligands, CD11b and CD18, were obtained from bone marrow cells of DBA/2j mice and adenovirally transduced with the IL-1ra gene or glucocerebrosidase (GC) gene ex vivo. In kidneys treated to develop UUO, levels of ICAM-1, IL-1β, and IL-1R expression increased within 3 days compared with contralateral untreated kidneys in the same mice. Similarly, the macrophage infiltration in the cortical interstitium increased after 3 days in UUO kidneys, but not untreated kidneys. After UUO developed, DBA/2j mice were injected i.v. with either IL-1ra+ vehicle cells (IL-1ra-treated mice) or GC+ vehicle cells (GC-treated mice) at 24 h after UUO. Six days after the injection of these vehicle cells, marked increase of CD11b+ IL-1ra+ vehicle cells was observed in the ICAM-1-positive interstitium of UUO kidneys from IL-1ra-treated mice. In contrast, no CD11b+ IL-1ra+ cells appeared in ICAM-1-negative contralateral kidneys from these mice. Furthermore, the infiltration of macrophages (p < 0.001), expression of ICAM-1 (p < 0.005), and presence of α-smooth muscle actin (p = 0.005) in the interstitium of UUO kidneys were significantly decreased in IL-1ra-treated mice compared with GC-treated mice. These findings suggest that IL-1 may contribute to the development of renal interstitial injury and that our method can deliver a functioning gene encoding an antiinflammatory cytokine gene specifically at that site by interacting with local adhesion molecules.

show abstract

“…26 Direct inhibition of ACE in a rat UUO model reduced monocyte/macrophage infiltration and ECM proteins accumulation. 123 It is interesting that in WT mice, the ACE inhibitorinduced reduction of TNFα and MCP-1 gene expression is higher than that achieved by combined AT1 and AT2 blockade. These data suggest a role for AngII-derived peptides, whose production is inhibited by ACE inhibitors, and that may be increased by the availability of AngII following AT1/2 blockade (Figure 1).…”

Section: Renin-angiotensin System (Ras)mentioning

confidence: 99%

Obstructive renal injury: from fluid mechanics to molecular cell biology

ORTIZ¹

2010

OAJU

View full text Add to dashboard Cite

Urinary tract obstruction is a frequent cause of renal impairment. The physiopathology of obstructive nephropathy has long been viewed as a mere mechanical problem. However, recent advances in cell and systems biology have disclosed a complex physiopathology involving a high number of molecular mediators of injury that lead to cellular processes of apoptotic cell death, cell injury leading to inflammation and resultant fibrosis. Functional studies in animal models of ureteral obstruction using a variety of techniques that include genetically modified animals have disclosed an important role for the renin-angiotensin system, transforming growth factor-β1 (TGF-β1) and other mediators of inflammation in this process. In addition, high throughput techniques such as proteomics and transcriptomics have identified potential biomarkers that may guide clinical decision-making.

show abstract

Enalapril reduces collagen type IV synthesis and expansion of the interstitium in the obstructed rat kidney

Cited by 203 publications

References 30 publications

TNF-α neutralization ameliorates obstruction-induced renal fibrosis and dysfunction

TNF-α neutralization ameliorates obstruction-induced renal fibrosis and dysfunction

Genetically Modified Bone Marrow-Derived Vehicle Cells Site Specifically Deliver an Anti-Inflammatory Cytokine to Inflamed Interstitium of Obstructive Nephropathy

Obstructive renal injury: from fluid mechanics to molecular cell biology

Contact Info

Product

Resources

About