Enamel epithelium expresses bone sialoprotein (BSP)

Abstract

HF: Enamel epithelium expresses hone sialoprolein ( 8 P). Eur J Oral Sci /99 : 106 (suppll ): 33 1 336. \.. Eur J Oral ci. 199Bone sialoprotein ( B P) is a major non-collagenou extracellular matrix protein in bone and other mineralized connective ti ues. B P i synthesized and secreted by bone-. dentin-and cementum-forming cells. In this study we hypothesized that B P may be also involved in enamel formation through it postulated role in matrix mineralization. In situ hybridization with cR A probes for rat and hamster BSP. respectively. showed strong mRNA signals in ameloblas ts actively synthesizing enamel matrix in developing incisors. However. no hybridization signals were observed at an earlier developmental tage when bell-shaped molar tooth germs were being formed . Immun ohistochemical analysis of tooth tis ue from transgenic mice harboring a 2.7 kb rat BSP promoter ligated to a lucifera e reporter gene revealed trong staining for luciferase in the enamel epithelium of the developing tooth germ . Interestingly. BSP expre ion was al ·o observed in epit helial cell of an ameloblastoma. The neoplastic epithelial nest and cords demonstrated strong mRNA signal to the human BSP probe while the connective tissue stroma showed only a background level of silver gra ins. Immune taining also howed deposition of B P by the odontogenic cells of the tumor. These results demon trate that B Pis expre sed by the enamel-forming epi thelium of developing teeth. sugge ting a pos ·ible role for B P in enamel formation and it subsequent mineraliza tion . xpre sion of the B P gene in ameloblastoma is consi tent with the expression of B P by the enamel epithelium and al o with the expression of BSP by neopla ·tic ti ·sues. suggesting a possible role in tumorigenesis.

Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells

“…1 G-L) in agreement with previous reports in vitro and in vivo (10,18). Expressional patterns of BSP protein analyzed by Western blot assay demonstrated that two bands were detected at positions, 39-and 27-kDa, respectively in the two mouse odontoblastic cells (Fig.…”

“…High BSP expression is restricted to the mineralized connective tissues and seen at the onset of bone, dentin and cementum formation, indicating a role of this protein in the initial mineralization of these tissues (10,(18)(19). OPN is a secreted adhesive glycoprotein expressed in both mineralized and non-mineralized tissues (20).…”

“…Also, these proteins contain an Arg-Gly-Asp (RGD) motif that mediates cell attachment/signaling via interaction with cell surface integrins and post-translational modification of phosphorylation and glycosylation. These SIBLING genes are highly expressed in mineralizing tissues related to tooth and bone development and mineralization (9)(10)(11)(12)(13)(14)(15)(16)(17).…”

Dentin Sialoprotein (DSP) Has Limited Effects on In Vitro Apatite Formation and Growth

“…Sialoproteins such as osteopontin (OPN), bone sialoprotein (BSP), and dentin sialoprotein (DSP) accumulate at the mineralization fronts in bone, dentin and other mineralizing tissues [1][2][3][4][5][6][7][8][9][10]. These proteins are part of an extended family of generally related secreted phosphoproteins, SIBLINGS (Small integrin-binding ligand, N-linked glycoproteinS), which share chromosomal location and general gene structure, but are not sequence related [11].…”

Molecular alterations in the development and progression of odontogenic tumors

“…Two dentin proteins, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), are tooth-specific. BSP, osteonectin, osteocalcin, and osteopontin are found in many types of odontogenic tumors, suggesting that these proteins play a role in pathological mineralization and/or tumor formation (114,(125)(126)(127). Expression of BMP, which induces bone and tooth formation, has been recognized in various types of odontogenic tumors (107)(108)128).…”