Enantiomer labelling, a method for the quantitative analysis of amino acids

Frank, Hartmut; Nicholson, G. J.; Bayer, Ernst

doi:10.1016/s0021-9673(00)91157-9

Cited by 213 publications

(78 citation statements)

References 9 publications

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“…Amino acids were separated as trifluoroacetyl-amino acid propylesters by gas chromatography on a glass capillary coated with ChirasiI-Val. The D-amino acid content was determined by enantiomer labelling (Frank et al, 1978). Amino acid analysis produced results according to the expected data, and no racemization could be detected.…”

Section: Methodsmentioning

confidence: 98%

Heterogeneity of linear B cell epitopes of the measles virus fusion protein reacting with late convalescent sera

Wiesmüller

Spahn²,

Handtmann³

et al. 1992

Journal of General Virology

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B cell epitopes of the measles virus fusion protein were mapped, by reacting sera from late convalescent donors with synthetic overlapping pentadecapeptides, segments covering the whole F protein sequence. When the regions of antibody binding were compared with the predicted secondary structure of the F protein, no detectable pattern became apparent. Exposed sequences as well as sequences hidden in the viral membrane or in the protein core of both the F1 and F2 polypeptides were recognized by the antibodies. The heterogeneity of the binding patterns was not merely dependent on the anti-measles virus titre. The importance of antibodies recognizing linear epitopes of the measles virus fusion protein for the immune protection is presently not known.

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Section: Methodsmentioning

confidence: 98%

Heterogeneity of linear B cell epitopes of the measles virus fusion protein reacting with late convalescent sera

Wiesmüller

Spahn²,

Handtmann³

et al. 1992

Journal of General Virology

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“…93,94 By adopting this method developed for (L) amino acids, a known quantity of the pure enantiomer can also be added to a racemic mixture to be quantified and the amount of the racemate originally present is then calculated from er determinated after the addition of the enantiomerically pure internal standard. In the absence of self-recognition between enantiomers in nonracemic mixtures (the EE effect), 95 the enantiomeric ratio er is not obscured by work-up, sample manipulations, or detection.…”

Section: Extending the Methods Of Enantiomer Labeling For The Quantitamentioning

confidence: 99%

Contributions to the theory and practice of the chromatographic separation of enantiomers

Schurig

2005

Chirality

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The theory and practice of enantioselective capillary chromatography employing metal coordination compounds and modified cyclodextrins as chiral stationary phases are treated. A unified approach involving all contemporary chromatographic methods and a single enantioselective column is described. Reliable thermodynamic data of enantioselectivity are derived by the retention-increment method. The existence of an isoenantioselective temperature is demonstrated. Kinetic enantiomerization studies are presented. The preparative-scale separation of enantiomers by gas chromatography with enantioselective packed columns is achieved. Unusual phenomena and future aspects of enantioselective chromatography are discussed.

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“…Aspartate enantiomers were quantified by gas chromatography on a capillary column coated with a chiral stationary phase (38)(39)(40). Isopropyl esters of the elastin hydrolysates were produced, followed by acylation with PFAA.…”

Section: Introductionmentioning

confidence: 99%

Marked longevity of human lung parenchymal elastic fibers deduced from prevalence of D-aspartate and nuclear weapons-related radiocarbon.

Shapiro

Endicott²,

Province³

et al. 1991

J. Clin. Invest.

576

367

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Normal structure and function of the lung parenchyma depend upon elastic fibers. Amorphous elastin is biochemically stable in vitro, and may provide a metabolically stable structural framework for the lung parenchyma. To test the metabolic stability of elastin in the normal human lung parenchyma, we have (a) estimated the time elapsed since the synthesis of the protein through measurement of aspartic acid racemization and (b) modeled the elastin turnover through'measurement ofthe prevalence of nuclear weapons-related "C. Elastin purified by a new technique from normal lung parenchyma was hydrolyzed; then the prevalences of D-aspartate and "4C were measured by gas chromatography and accelerator-mass spectrometry, respectively. D-aspartate increased linearly with age; K,,.,p (1.76x 10-3yr-') was similar to that previously found for extraordinarily stable human tissues, indicating that the age of lung parenchymal elastin corresponded with the age of the subject. Radiocarbon prevalence data also were consistent with extraordinary metabolic stability of elastin; the calculated mean carbon residence time in elastin was 74 yr (95% confidence limits, 40-174 yr). These results indicate that airspace enlargement characteristic of "aging lung" is not associated with appreciable new synthesis of lung parenchymal elastin. The present study provides the first tissue-specific evaluation of turnover of an extracellular matrix component in humans and underscores the potential importance of elastin for maintenance of normal lung structure. Most importantly, the present work provides a foundation for strategies to directly evaluate extracellular matrix injury and repair in diseases of lung (especially pulmonary emphysema), vascular tissue, and skin. (J. Clin. Invest. 1991.

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Enantiomer labelling, a method for the quantitative analysis of amino acids

Cited by 213 publications

References 9 publications

Heterogeneity of linear B cell epitopes of the measles virus fusion protein reacting with late convalescent sera

Heterogeneity of linear B cell epitopes of the measles virus fusion protein reacting with late convalescent sera

Contributions to the theory and practice of the chromatographic separation of enantiomers

Marked longevity of human lung parenchymal elastic fibers deduced from prevalence of D-aspartate and nuclear weapons-related radiocarbon.

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