Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine

Abstract

Enantiomer‐specific high‐performance liquid chromatography with fluorescence detection using 4‐(4,5‐diphenyl‐1H‐imidazol‐2‐yl)‐benzoyl chloride as a fluorescence labeling reagent was applied to determine methamphetamine and its metabolites in abusers’ hair and urine. Hair samples were segmentally analyzed based on 1 cm long segments. In four hair samples, only the S(+)‐enantiomers of methamphetamine and its N‐demethylated metabolite, S(+)‐amphetamine were detected. Satisfactory correlation (r = 0.901) between the results of high‐performance liquid chromatography‐fluorescence and those of gas chromatography‐nitrogen phosphorous detection was obtained (n = 19). In an abuser's urine sample, the S(+)‐ and R(−)‐enantiomers of methamphetamine, amphetamine and para‐hydroxymethamphetamine were detected. The degree of N‐demethylation of S(+)‐methamphetamine into the corresponding metabolite of amphetamine was significantly higher than that of the R(−)‐enantiomer. Copyright © 1999 John Wiley & Sons, Ltd.

High throughput chiral analysis of urinary amphetamines by GC-MS using a short narrow-bore capillary column

“…For chiral analysis of a compound, MS analysis is generally powerless, because both enantiomers of a target compound give almost the same mass spectra; it is essential to completely separate the enantiomers by chromatography. Although chromatographic separation of enantiomers by HPLC has been reported [1], the Table 1 Validation data for enantiomers of amphetamines spiked into human urine measured by the present high throughput gas chromatography-mass spectrometry method number of theoretical plates is much higher in GC analysis than in HPLC analysis. For chiral analysis of a drug, GC-MS seems much more advantageous than liquid chromatography (LC)-MS (-MS), because of the above higher separation ability.…”

“…Chiral analyses of amphetamine-type stimulants (ATSs) in human samples have been widely performed, utilizing high-performance liquid chromatography (HPLC) [1], capillary electrophoresis [2], and gas chromatographymass spectrometry (GC-MS) [3]. The importance of such analysis has increased in recent years.…”

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

“…Thereupon, HPLC-FL methods using DIB-Cl for the chiral quantification of MP and AP in urine and hair were developed. [65][66][67] The enantioseparation of DIB-labels was achieved on a Chiralcel OD-RH column with a mixture of acetonitrile and phosphate-citrate buffer containing NaPF6 as a mobile phase. Chiral analyses of MP and AP by using urine samples collected from a patient who received selegiline as an anti-Parkinson's disease medicament and from suspected MP abusers were performed.…”

New trends in hair analysis and scientific demands on validation and technical notes

“…Enantioselective determination procedures in hair specimens have mainly been described for methadone and its main metabolite [121][122][123] as well as for amphetamine, methamphetamine and designer amphetamines [124][125][126][127][128]. Together with the development of novel enantioselective procedures the enantioselective determination of special analytes in hair specimens opens up the possibility to conduct further research, e.g.…”