Encapsulation of Gold Nanoparticles in a DNA Origami Cage

Zhao, Zhao; Jacovetty, Erica L.; Liu, Yan

doi:10.1002/anie.201006818

Cited by 139 publications

(80 citation statements)

References 32 publications

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“…In step 2, to attach the Au “seed” to the DNA barrel (36), twenty-seven 21-nucleotide (nt) single-stranded (ss) DNA “handles” were immobilized in the interior surface of the barrel (Fig. 1A).…”

Section: Casting a Ag Cuboidmentioning

confidence: 99%

Casting inorganic structures with DNA molds

Sun

Boulais

Hakobyan

et al. 2014

Science

277

288

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We report a general strategy for designing and synthesizing inorganic nanostructures with arbitrarily prescribed three-dimensional shapes. Computationally designed DNA strands self-assemble into a stiff “nano-mold” that contains a user-specified three-dimensional cavity and encloses a nucleating gold “seed”. Under mild conditions, this seed grows into a larger cast structure that fills and thus replicates the cavity. We synthesized a variety of nanoparticles with three nanometer resolution: three distinct silver cuboids with three independently tunable dimensions, silver and gold nanoparticles with diverse cross sections, and composite structures with homo-/heterogeneous components. The designer equilateral silver triangular and spherical nanoparticles exhibited plasmonic properties consistent with electromagnetism-based simulations. Our framework is generalizable to more complex geometries and diverse inorganic materials, offering a range of applications in biosensing, photonics, and nanoelectronics.

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Section: Casting a Ag Cuboidmentioning

confidence: 99%

Casting inorganic structures with DNA molds

Sun

Boulais

Hakobyan

et al. 2014

Science

277

288

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“…We chose such short additional length because oligonucleotides attached to a gold nanoparticle can be pushed and squeezed upon mechanical restrictions reducing dramatically the hydrodynamic diameter of the oligonucleotide-GNP particles. [21,32] Overall, sizes Σ = 2·(r + s) of 9 nm and 14 nm were used . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 …”

Section: B Model Detailsmentioning

confidence: 99%

DNA‐Origami‐Driven Lithography for Patterning on Gold Surfaces with Sub‐10 nm Resolution

et al. 2017

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The programmability [1] and self-assembly properties of DNA provides means of precise organization of matter at the nanoscale. [2] DNA origami allows the folding of DNA into twodimensional [3] and three-dimensional [4] structures, and has been used to organize biomolecules, [2b, e, 5] nanophotonic [2a, c, f, 6] and electronic [7] components with a resolution of 6 nm / pixel. [8] Two-dimensional DNA origami has been also used as a platform to organize other chemical [9] species that can then be placed on technologically relevant substrates. [2c, 10] Nevertheless, these approaches have only used the DNA nanostructure to hold the chemical species on the surface and, to the best of our knowledge, have never been utilized to immobilize nucleic acids patterns on surfaces with sub-10 nm resolution providing an enable 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Submitted to 2 platform for potential applications such as multiplexed biochemical assays. [11] to the creation of metasurfaces [12] with potentially reconfigurable features. Revised ManuscriptHerein we report on the use of a two-dimensional DNA origami as a template to covalently attach DNA with a pre-programmed pattern on a surface (see Scheme 1). The method utilizes the incorporation of modified staple strands in programmed positions of the DNA origami (DNA origami stamp), acting as DNA ink. Once the DNA origami is immobilized on the surface, the modified staples can react with the surface creating a defined DNA pattern (Stamping step). The pattern can then be exposed upon denaturation of the DNA origami stamp (Unmasking step), allowing the non-bound staples to be rinsed off of the surface. As a proof-of-principle of this methodology, we have created a linear pattern of thiolmodified DNA ink on gold surfaces. The formation of the linear pattern was revealed by the successful formation of bead-on-a-string-like structures (here named "chains" for simplicity) composed of gold nanoparticles conjugated with thiol-oligonucleotides (OGNP) that are hybridized to the DNA ink pattern (Development step). The linear pattern provided a direct evidence of the stamping process and was chosen as a simple geometry that can be statistically analysed in our experimental setup. Montecarlo Simulations have been used for better understanding of our statistical results and to determine key elements governing the process that can be used for future optimization of pattern information transfer with DNA origami stamp methodology. Furthermore, we have studied the development of more complex patterns using Montecarlo Simulations.We demonstrate that our approach can be employed to form DNA patterns with sub-10 nm resolution to flat gold surfaces, an unsolved goal to date. This methodology can thus be extended to other surfaces utilizing different covalent strategies. [10b, 13] M...

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“…[11] Efforts have been made to control the density and orientation of DNAs trands on AuNPs, [12] which have proven useful in aw ide range of applications,i ncluding diagnosis,d rug delivery,b ioimaging, catalysis,and metamaterials. [14] However,whereas nearly perfect valence control has been realized, bond anisotropy is often poorly defined in these procedures.V ery recently,S leiman et al proposed as trategy in which DNAp atterns were transferred from DNAframework nanostructures onto 10 nm AuNPs. [14] However,whereas nearly perfect valence control has been realized, bond anisotropy is often poorly defined in these procedures.V ery recently,S leiman et al proposed as trategy in which DNAp atterns were transferred from DNAframework nanostructures onto 10 nm AuNPs.…”

mentioning

confidence: 99%

Transfer of Two‐Dimensional Oligonucleotide Patterns onto Stereocontrolled Plasmonic Nanostructures through DNA‐Origami‐Based Nanoimprinting Lithography

et al. 2016

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The precise functionalization of self-assembled nanostructures with spatial and stereocontrol is am ajor objective of nanotechnology and holds great promise for many applications.H erein, the nanoscale addressability of DNAo rigami was exploited to develop ap recise copymachine-like platform that can transfer two-dimensional oligonucleotide patterns onto the surface of gold nanoparticles (AuNPs) through ad eliberately designed toehold-initiated DNAd isplacement reaction. This strategy of DNA-origamibased nanoimprinting lithography (DONIL) demonstrates high precision in controlling the valence and valence angles of AuNPs.These DNA-decorated AuNPs act as precursors in the construction of discrete AuNP clusters with desired chirality.

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Encapsulation of Gold Nanoparticles in a DNA Origami Cage

Cited by 139 publications

References 32 publications

Casting inorganic structures with DNA molds

Casting inorganic structures with DNA molds

DNA‐Origami‐Driven Lithography for Patterning on Gold Surfaces with Sub‐10 nm Resolution

Transfer of Two‐Dimensional Oligonucleotide Patterns onto Stereocontrolled Plasmonic Nanostructures through DNA‐Origami‐Based Nanoimprinting Lithography

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