Encapsulation of green tea polyphenol nanospheres in PVA/alginate hydrogel for promoting wound healing of diabetic rats by regulating PI3K/AKT pathway

Chen, Guan-Yu; He, Libang; Zhang, Peng; Zhang, Jie; Mei, Xifan; Wang, Dahao; Zhang, Yiyao; Ren, Xiuli; Chen, Zhenhua

doi:10.1016/j.msec.2020.110686

Cited by 94 publications

(50 citation statements)

References 35 publications

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“…The clearest trend in microvascular endothelial cells was the significant enrichment of DEGs in the immune and endocrine system pathways, such as the phosphatidylinositol 3-hydroxy kinase/ protein kinase B (PI3K/AKT) signaling pathway. PI3K/AKT signaling is significant in the normal wound healing process and plays a vital role in the proliferation, regeneration, remodeling, and reepithelialization of injured tissue (28)(29)(30). In addition, much evidence points to a critical role of the PI3K/AKT pathway in wound healing in diverse pathological states, including hyperglycemia (31,32).…”

Section: Discussionmentioning

confidence: 99%

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Zhang

Yang

et al. 2021

Front. Endocrinol.

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Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients’ quality of life and cause long-lasting disability. However, the cellular and molecular mechanisms linking diabetes-related hyperglycemia and skin complications are still incompletely understood. To assess the role of the various skin-cell types in hyperglycemia-induced skin disorders, we performed RNA sequencing-based transcriptome analysis, measuring gene expression patterns in biological replicates in normal- and high glucose-stimulated skin cells. Three primary human skin-cell types were examined, i.e., epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells. For each separate cell type, we identified gene expression. Comparing gene abundances and expression levels revealed that transcription profiles exhibit distinct patterns in the three skin-cell types exposed to normal (i.e., physiological) glucose treatment and high (i.e., supraphysiological) glucose treatment. The obtained data indicate that high glucose induced differential gene expression and distinct activity patterns in signaling pathways in each skin-cell type. We are adding these data to the public database in the hope that they will facilitate future studies to develop novel targeted interventions for diabetic skin complications.

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Section: Discussionmentioning

confidence: 99%

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Zhang

Yang

et al. 2021

Front. Endocrinol.

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“…The advanced wound care study has shown that application of high concentrations of polyphenols to wound repair has antibacterial and cytokine-release inhibition effects [ 41 , 42 ]. In addition, the chitosan polymer, incorporated as a hydrogel backbone, has been applied for wound care with antibacterial effects and exhibited biocompatibility [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Enzyme-mediated one-pot synthesis of hydrogel with the polyphenol cross-linker for skin regeneration

Kim

et al. 2020

Materials Today Bio

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Polyphenols can trigger immunity that activates intracellular anti-inflammatory signaling and prevents external infections. In this study, we report the fabrication of chitosan-based hydrogels with epigallocatechin gallate (EGCG) using enzyme-mediated one-pot synthesis. The tyrosinase-mediated oxidative reaction of the phenolic rings of EGCG with the primary amines on chitosan results in stable EGCG-chitosan hydrogels. The EGCG concentrations contributed to the cross-linking density and physical properties of EGCG-chitosan hydrogels. Furthermore, EGCG-chitosan hydrogels maintained intrinsic properties such as antibacterial and antioxidant effects. When endotoxin-activated RAW 264.7 macrophage cells were cultured with EGCG-chitosan hydrogels, the hydrogels reduced the inflammatory response of the RAW 264.7 cells. Furthermore, subcutaneous implantation of EGCG-chitosan hydrogels reduced endogenous macrophage and monocyte activation. When the EGCG-chitosan hydrogels were applied to a full-skin defect wound, they facilitated skin regeneration. Our study demonstrates that the one-pot synthesized EGCG-chitosan hydrogels can be applied in broad tissue regeneration applications that require immune modulation.

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“…Resveratrol-polypeptide-hydrogel nanoparticles are not cytotoxic and inhibit the macrophage release of pro-inflammatory factors to accelerate wound healing [ 247 ]. Animal experiments and molecular mechanism studies have shown that PVA/alginate hydrogel particles coated with tea polyphenols can promote wound healing in diabetic rats by regulating the PI3K/AKT signaling pathway [ 248 ]. Similarly, a newly developed hydrogel based on plant polyphenols, tannins and polypyrrole chains can stimulate tissue repair after bone marrow injury [ 249 ].…”

Section: Nanoformulations For Loading and Delivery Of Polyphenolsmentioning

confidence: 99%

Nanoformulations to Enhance the Bioavailability and Physiological Functions of Polyphenols

et al. 2020

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Polyphenols are micronutrients that are widely present in human daily diets. Numerous studies have demonstrated their potential as antioxidants and anti-inflammatory agents, and for cancer prevention, heart protection and the treatment of neurodegenerative diseases. However, due to their vulnerability to environmental conditions and low bioavailability, their application in the food and medical fields is greatly limited. Nanoformulations, as excellent drug delivery systems, can overcome these limitations and maximize the pharmacological effects of polyphenols. In this review, we summarize the biological activities of polyphenols, together with systems for their delivery, including phospholipid complexes, lipid-based nanoparticles, protein-based nanoparticles, niosomes, polymers, micelles, emulsions and metal nanoparticles. The application of polyphenol nanoparticles in food and medicine is also discussed. Although loading into nanoparticles solves the main limitation to application of polyphenolic compounds, there are some concerns about their toxicological safety after entry into the human body. It is therefore necessary to conduct toxicity studies and residue analysis on the carrier.

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Encapsulation of green tea polyphenol nanospheres in PVA/alginate hydrogel for promoting wound healing of diabetic rats by regulating PI3K/AKT pathway

Cited by 94 publications

References 35 publications

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Enzyme-mediated one-pot synthesis of hydrogel with the polyphenol cross-linker for skin regeneration

Nanoformulations to Enhance the Bioavailability and Physiological Functions of Polyphenols

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