Encapsulation of Polyphenols from Lycium barbarum Leaves into Liposomes as a Strategy to Improve Their Delivery

Abstract

This study is focused on the encapsulation of polyphenols from Lycium barbarum leaves into liposomes as a strategy to improve their delivery. Liposomes loaded with Lycium barbarum leaves extract were obtained and characterized for particle size, polydispersity, entrapment efficiency, and stability. Liposomes presented entrapment efficiency higher than 75%, nanometric particle size, narrow polydispersity, and good stability over three months at 4 °C. The liposomes containing Lycium barbarum offered a slower release of polyphenols with attenuated burst effect compared with the dissolution of free Lycium barbarum extract in phosphate buffer solution at pH 7.4. Moreover, an in vitro pretreatment of 24 h with loaded liposomes showed a cytoprotective effect against H2O2-induced cytotoxicity on L-929 mouse fibroblasts cells. These preliminary findings imply that liposomes could be successfully employed as carriers for polyphenols in pharmaceutical applications.

Development, Characterization and Incorporation of Alginate-Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies

“…Further, Yücel et al [ 39 ] reported liposomal EE of 62% for rosmarinic acid prepared by the dry film hydration method using dipalmitoyl phosphatidylcholine and cholesterol. Liposomes were also found to be an appropriate encapsulation technique for Polygonum aviculare L. extract [ 40 ], Glycyrrhiza glabra L. roots extract [ 41 ], and Lycium barbarum L. [ 42 ] leaves extract, achieving EE of 83, 84 and 84.6%, respectively.…”

Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review

“…Polyphenols from goji berries’ leaves have been encapsulated in liposomes to improve their delivery in terms of sustaining their release, and avoiding the burst effect compared to the dissolution of the free extract [ 151 ]. The obtained liposomes have successfully served as carriers for polyphenols and demonstrated a cytoprotective effect on L-929 mouse fibroblasts cells.…”

Phytochemicals and bioactivities of Goji (Lycium barbarum L. and Lycium chinense Mill.) leaves and their potential applications in the food industry: a review

“…Limitations come into being if employing extracts from Goji leaves directly due to the poor solubility, toxicity and degradation in a gastrointestinal medium (Pavaloiu et al ., 2021). To deal with this, one of the strategies is to encapsulate the extracts into emerging drug delivery systems (Yadav et al ., 2014).…”

“…Pavaloiu et al . (2021) prepared the liposomes loaded with polyphenolic extracts from LBL. Liposome encapsulation promoted the delivery of LBL extracts.…”