Encapsulation of viable cells within polyacrylate membranes

Gharapetian, Hrire M.; Davies, Nathan; Sun, Anthony M.

doi:10.1002/bit.260281019

Cited by 37 publications

(13 citation statements)

References 10 publications

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“…In principle, animal cells can be cultivated in immobilization systems, where the cells are encapsulated [113][114][115][116][117] or entrapped in a polymer matrix, such as agarose [118] or alginate [119]. These capsules or beads are then cultivated in the form of suspensions in stirred tank reactors.…”

Section: Other Immobilization Systemsmentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

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Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for treatment of acquired or inherited diseases. In this review, the present state-of-the-art of the production of retroviral vectors and the most important parameters, such as the choice of the producer cell line, stability issues, medium additives, serum, type of bioreactor, that influence production issues is presented and discussed in light of an optimal vector production. The available literature data clearly indicate that, on one hand, the choice of the producer cell line is of utmost importance for obtaining a high level producer cell line, and that, on the other hand, the optimization of the medium, e.g. the replacement of glucose by fructose, has a potential for improving vector production rates and titers. Finally, the use of high-density perfusion culture systems for adherent as well as for suspension cells presents the best choice for a production system, because high cell densities imply high reactor specific production rates, which must be associated with a rapid harvest of the produced vector, thus avoiding vector inactivation due to an extended residence time. The overall optimization of the cultivation and production parameters will have a significant impact on the use of retroviral vectors for gene therapy purposes.

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Section: Other Immobilization Systemsmentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

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“…Culture of mammalian cells for the production of biologicals have used methods such as batch, fed-batch, chemostat or perfusion cultures (Feder, 1983(Feder, , 1985, hollow fibers (Von Wedel, 1985; Knazek, 1972), microcapsules (Jarvis, 1983;Gharapetian, 1986;Sefton, 1987), rotating filters (Himmelfarb, 1969;Tolbert, 1981), airlift fermentors (Katinger, 1979; Arathoon, 1986), etc.. We consider that an important point for industrial-scale mammalian cell culture systems are ease of scale up and handling, high cell density and high concentration of products. But no culture system which satisfies all of these conditions has been reported so far.…”

Section: Introductionmentioning

confidence: 99%

High cell density perfusion culture of hybridoma cells recycling high molecular weight components

et al. 1988

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We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.

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“…Toxic effects arising out of direct contact with charged polymers may affect the viability and growth of animal cells (Gharapetian et al;. Charged polyelectrolytes can also interfere with the functioning of bioactive materials such as enzymes by complexing with multivalent ions which may serve as cofactors.…”

Section: Resultsmentioning

confidence: 99%