Encephalitis in Mice Inoculated Intranasally with an Influenza Virus Strain Originated from a Water Bird.

Shinya, Kyoko; Silvano, Florence D.; Morita, Takehito; Shimada, Akinori; Nakajima, Masahide; Ito, Toshihiro; Otsuki, Koichi; Umemura, Takashi

doi:10.1292/jvms.60.627

Cited by 18 publications

(16 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present results of both histological and immunohistochemical examinations on the CNS of mice were consistent with those of our previous reports in which the virus invaded the brain stem after replicating in the respiratory mucosa [14,15]. Treatment of the mice with the antipyretics did not affect the development of the CNS lesions.…”

Section: Discussionsupporting

confidence: 93%

“…The organs were sectioned at a thickness of 4-µm and stained with hematoxylim and eosin (HE) for light microscopic examination. For detection of avian influenza virus antigens in the tissues, all the sections were stained using the streptavidin-biotin immunoperoxidase complex method (Histofine SAB-PO kit, Nichirei Corp., Tokyo) using rabbit anti-strain 499 [A/Whistling swan/Shimane/499/83 (H5N3)] hyperimmune serum [12,15] at a 1:2,000 dilution as the primary antibody.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

“…We previously demonstrated that nasal inoculation of a neurovirulent avian influenza virus, strain 24a5b that acquired virulence for chicks and mice through serial passages in air sac and brain of chicks, caused lethal encephalitis in mice and chicks. In the murine model, the virus invades the CNS via vagus nerves after replicating in the respiratory mucosa [14,15], whereas in the chick model, it spreads hematogenously to the CNS [12,16]. It would be intriguing to investigate the effect of antipyretics on the murine and poultry models of influenzal encephalitis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Effects of Antipyretics on Influenza Virus Encephalitis in Mice and Chicks

Sunden

Park

Matsuda

et al. 2003

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. To investigate the effect of antipyretics on the murine and poultry models of influenzal encephalitis, we injected large quantities of antipyretics, acetylsalicylic acid (aspirin) and dicrofenac sodium (voltaren). The effect of antipyretic treatment on the murine encephalitis model was unremarkable histologically and immunohistochemically. Whereas in chicks, CNS lesions consisting of perivascular cuffing and gliosis appeared only in those animals treated with the antipyretics and viral antigen was detected mai nly in the nuclei of glial cells or vascular endothelia of voltaren-treated animals. We here demonstrate that antipyretic treatment aggravated the hematogenous spread of the influenza virus to the CNS in chicks, but did not affect the transneural infection in mice.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The Effects of Antipyretics on Influenza Virus Encephalitis in Mice and Chicks

Sunden

Park

Matsuda

et al. 2003

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…5) indicated that these neurotropic MB variants had spread from the lungs to the brain trans-synaptically, via sensory nerves. A neuronal route of viral transmission has also been shown in mice infected with avian H5N3 viruses that adapted in chickens and became highly virulent (25,26). The virus of genotype B replicated efficiently in mouse lungs and caused some mortality in infected mice (Table 1, Fig.…”

Section: Sequence Comparison Of Original Viruses and Mb Variantsmentioning

confidence: 83%

Neurovirulence in Mice of H5N1 Influenza Virus Genotypes Isolated from Hong Kong Poultry in 2001

Lipatov¹,

Krauss²,

Guan

et al. 2003

J Virol

View full text Add to dashboard Cite

We studied the pathogenicity of five different genotypes (A to E) of highly pathogenic avian H5N1 viruses, which contained HA genes similar to those of the H5N1 virus A/goose/Guangdong/1/96 and five different combinations of "internal" genes, in a mouse model. Highly pathogenic, neurotropic variants of genotypes A, C, D, and E were isolated from the brain after a single intranasal passage in mice. Genotype B virus was isolated from lungs only. The mouse brain variants had amino acid changes in all gene products except PB1, NP, and NS1 proteins but no common sets of mutations. We conclude that the original H5N1/01 isolates of genotypes A, C, D, and E were heterogeneous and that highly pathogenic neurotropic variants can be rapidly selected in mice.

show abstract

“…Influenza virus antigens have also been detected in glial cells and neurocytes in mouse models of encephalitis (5)(6)(7). Besides, proinflammatory cytokines are reportedly increased in the CSF and plasma of patients with influenza encephalopathy and encephalitis (1,4,7,8).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis and Proinflammatory Cytokine Responses of Primary Mouse Microglia and Astrocytes Induced by Human H1N1 and Avian H5N1 Influenza Viruses

Wang

Zhang

et al. 2008

Cell Mol Immunol

View full text Add to dashboard Cite

Patients with an influenza virus infection can be complicated by acute encephalopathy and encephalitis. To investigate the immune reactions involved in the neurocomplication, mouse microglia and astrocytes were isolated, infected with human H1N1 and avian H5N1 influenza viruses, and examined for their immune responses. We observed homogeneously distributed viral receptors, sialic acid (SA)-α2,3-Galactose (Gal) and SA-α2,6-Gal, on microglia and astrocytes. Both viruses were replicative and productive in microglia and astrocytes. Virus-induced apoptosis and cytopathy in infected cells were observed at 24 h post-infection (p.i.). Expression of IL-1β, IL-6 and TNF-α mRNA examined at 6 h and 24 h p.i. was up-regulated, and their expression levels were considerably higher in H5N1 infection. The amounts of secreted proinflammatory IL-1β, IL-6 and TNF-α at 6 h and 24 h p.i. were also induced, with greater induction by H5N1 infection. This study is the first demonstration that both human H1N1 and avian H5N1 influenza viruses can infect mouse microglia and astrocytes and induce apoptosis, cytopathy, and proinflammatory cytokine production in them in vitro. Our results suggest that the direct cellular damage and the consequences of immunopathological injury in the CNS contribute to the influenza viral pathogenesis. Cellular & Molecular Immunology. 2008;5(2):113-120.

show abstract

Encephalitis in Mice Inoculated Intranasally with an Influenza Virus Strain Originated from a Water Bird.

Cited by 18 publications

References 11 publications

The Effects of Antipyretics on Influenza Virus Encephalitis in Mice and Chicks

The Effects of Antipyretics on Influenza Virus Encephalitis in Mice and Chicks

Neurovirulence in Mice of H5N1 Influenza Virus Genotypes Isolated from Hong Kong Poultry in 2001

Apoptosis and Proinflammatory Cytokine Responses of Primary Mouse Microglia and Astrocytes Induced by Human H1N1 and Avian H5N1 Influenza Viruses

Contact Info

Product

Resources

About