Encoded hydrogel microparticles with universal mismatch-incorporated DNA probes for highly specific multiplex detection of SNPs

Moon, Hyun June; Mun, Seok Joon; Lee, Jun Ho; Roh, Yoon Ho; Lim, Yong Jun; Bong, Ki Wan

doi:10.1016/j.talanta.2022.123480

Talanta

2022

DOI: 10.1016/j.talanta.2022.123480

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Encoded hydrogel microparticles with universal mismatch-incorporated DNA probes for highly specific multiplex detection of SNPs

Hyun June Moon

Seok Joon Mun

Jun Ho Lee

et al.

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Cited by 4 publications

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Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling

Roh,

Morales,

Huynh

et al. 2024

Small

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Extracellular vesicles (EVs) are promising for molecular diagnostics, but current analyses are limited by the rarity and compositional heterogeneity of EV protein expression. Therefore, single EV profiling methods require high sensitivity, multiplexing, and throughput to address these issues. Here a single EV analysis technique that utilizes squeezable methacrylated hyaluronic acid hydrogel microparticles (MHPs) is described as a scaffold to immobilize EVs and perform an integrated rolling circle amplification (RCA) assay for an ultra‐sensitive and multiplex analysis of single EV proteins. EVs are prepared into MHPs in a high‐throughput manner with droplet microfluidics and optimally labeled with antibody‐oligonucleotide conjugates in MHPs without steric limitations. By designing MHPs with high compressibility, single EV protein signals are amplified as RCA products that can be aligned on the same plane by physically squeezing MHPs and visualized with low magnification. This method provides a simple and scalable single EV imaging analysis pipeline for identifying multiplex marker expression patterns from single EVs. For validation, the single EV heterogeneity of highly expressed cancer cell markers is profiled across different cancer cell lines. These findings exemplify squeezable MHPs as a robust platform with high sensitivity, multiplexing, and scalability for resolving single EV heterogeneity and advancing molecular assay technologies.

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Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling

Roh,

Morales,

Huynh

et al. 2024

Small

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Current status and trends in small nucleic acid drug development: Leading the future

Miao,

Fu,

et al. 2024

Acta Pharmaceutica Sinica B

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Multiplex genotyping of SNPs in genomic DNA via hydrogel-based assay mediated with MutS and polyethylene glycol

Mun,

Jang,

Park

et al. 2023

Biosensors and Bioelectronics

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Encoded hydrogel microparticles with universal mismatch-incorporated DNA probes for highly specific multiplex detection of SNPs

Cited by 4 publications

References 50 publications

Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling

Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling

Current status and trends in small nucleic acid drug development: Leading the future

Multiplex genotyping of SNPs in genomic DNA via hydrogel-based assay mediated with MutS and polyethylene glycol

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