A gronomy J our n al • Volume 10 9, I ssue 5 • 2 017 T he end-of-season CSNT was developed in Iowa by Binford et al. (1990) as a tissue test for grain corn, with samples taken at physiological maturity for distinguishing between fertilizer N applications that resulted in a crop yield increase and those that exceeded crop needs. Binford et al. (1990) showed that CSNT-N increased linearly with N fertilizer rate when N was applied beyond what was needed for maximum yield. A critical CSNT-N of 250 mg NO 3 -N kg -1 was established, based on a linear-to-plateau crop yield response model with relative corn yield as the dependent variable.Two years after the introduction of the CSNT, the optimum CSNT range was adjusted (700 to 2000 mg NO 3 -N kg -1) ) to identify deficiencies or excesses of N (Binford et al., 1992b). Similarly, Blackmer and Mallarino (1996) introduced four sufficiency categories including 250 and 700 mg NO 3 -N kg -1 defined as marginal and 700 to 2000 mg NO 3 -N kg -1 as optimal. The marginal category was described as the range in which N availability was very close to the minimal amounts needed, close enough to not be a concern, but too close to be a target for optimal N management (Blackmer and Mallarino, 1996).The initial studies in Iowa were followed by studies in other regions. Sims et al. (1995) and Varvel et al. (1997), working in Delaware and Nebraska, respectively, also concluded the CSNT was successful in separating essential vs. excessive fertilizer N applications. Studies by Fox et al. (2001) confirmed the usefulness of the CSNT in Pennsylvania and suggested that the original critical value of 250 mg NO 3 -N kg -1 resulted in a lower error rate (incorrect classification) than the value of 700 mg NO 3 -N kg -1 suggested in 1992.With the expansion of use of the CSNT, changes in methodologies were introduced. In the original work in Iowa, the NO 3 -N content was determined in 20-cm long corn stalk sections, with dry leaves removed, cut at 15 and 35 cm aboveground (Binford et al., 1990). This field sampling method has served as the convention for sampling for the majority of subsequent studies and is what is recommended currently to farmers by many Land Grant universities. Searching for a method to reduce the amount of plant material to be collected, dried, and ground, Isla and Blackmer (2007)
ABSTRACTThe corn (Zea mays L.) stalk nitrate test (CSNT) is a site-specific, after-the-fact, diagnostic tool for N management of corn. Although considered effective, widespread adoption of CSNT is limited due to somewhat impractical sample collection, handling, and laboratory sample processing protocols. Five studies were conducted to evaluate the impact of alternative sampling, sample handling, and laboratory processing protocols on CSNT outcomes. Study 1 evaluated the effect of stalk length and position on CSNT values. In Study 2 timing of sampling or growth stage was evaluated. In Study 3 several sampling patterns within a field (samples taken within a corn row vs. perpendicular or under an angle to r...