Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes

Liu, Wanxin; Zhao, Qi; Piao, Shan-Hua; Chun-sheng, Wang; Kong, Qingran; Tie-zhu, AN

doi:10.1071/rd16498

Cited by 9 publications

(7 citation statements)

References 42 publications

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“…Together, these ribonucleoproteins form the RNA‐induced silencing complex (RISC) . The endoribonuclease activity of Ago2 cleaves the target RNA to achieve post‐transcriptional repression, while other Argonaute proteins repress expression through non‐degradative mechanisms.…”

Section: Challenges and Strategies In Targeting Multifunctional Host mentioning

confidence: 99%

Harnessing host–virus evolution in antiviral therapy and immunotherapy

Heaton

2019

Clin & Trans Imm

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Pathogen resistance and development costs are major challenges in current approaches to antiviral therapy. The high error rate of RNA synthesis and reverse‐transcription confers genome plasticity, enabling the remarkable adaptability of RNA viruses to antiviral intervention. However, this property is coupled to fundamental constraints including limits on the size of information available to manipulate complex hosts into supporting viral replication. Accordingly, RNA viruses employ various means to extract maximum utility from their informationally limited genomes that, correspondingly, may be leveraged for effective host‐oriented therapies. Host‐oriented approaches are becoming increasingly feasible because of increased availability of bioactive compounds and recent advances in immunotherapy and precision medicine, particularly genome editing, targeted delivery methods and RNAi. In turn, one driving force behind these innovations is the increasingly detailed understanding of evolutionarily diverse host–virus interactions, which is the key concern of an emerging field, neo‐virology. This review examines biotechnological solutions to disease and other sustainability issues of our time that leverage the properties of RNA and DNA viruses as developed through co‐evolution with their hosts.

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Section: Challenges and Strategies In Targeting Multifunctional Host mentioning

confidence: 99%

Harnessing host–virus evolution in antiviral therapy and immunotherapy

Heaton

2019

Clin & Trans Imm

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“…Deep sequencing of small RNAs has identified miRNAs, piRNAs, and a large number of siRNAs in mammalian oocytes (Tam et al ., 2008; Watanabe et al ., 2006, 2008). Studies in mammals indicate that siRNA function is essential during oocyte meiosis (Liu et al ., 2017 a ; Stein et al ., 2015). RNase III Dicer is required for both miRNA and siRNA biogenesis.…”

Section: Regulation Of Maternal Mrna Stabilitymentioning

confidence: 99%

“…RNase III Dicer is required for both miRNA and siRNA biogenesis. Dicer depletion in oocytes causes misregulated transcripts and meiotic defects such as abnormal spindle formation and chromosomal organization (Liu et al ., 2010; Liu et al ., 2017 a ; Suh et al ., 2010; Murchison et al ., 2007). However, miRNA function appears to be dispensable for oocyte maturation, at least in mammals (Suh et al ., 2010; Ma et al ., 2010; Liu et al ., 2017 a ).…”

Section: Regulation Of Maternal Mrna Stabilitymentioning

confidence: 99%

“…Dicer depletion in oocytes causes misregulated transcripts and meiotic defects such as abnormal spindle formation and chromosomal organization (Liu et al ., 2010; Liu et al ., 2017 a ; Suh et al ., 2010; Murchison et al ., 2007). However, miRNA function appears to be dispensable for oocyte maturation, at least in mammals (Suh et al ., 2010; Ma et al ., 2010; Liu et al ., 2017 a ). In mice and pigs, depletion of DiGeorge syndrome critical region 8 (DGCR8, a key component of miRNA biogenesis) in oocytes did not change the oocyte transcriptome and these oocytes undergo normal meiotic maturation, fertilization, and embryonic development (Suh et al ., 2010; Liu et al ., 2017 a ).…”

Section: Regulation Of Maternal Mrna Stabilitymentioning

confidence: 99%

“…In addition, timely elimination of maternal mRNAs is also crucial for proper oocyte‐to‐zygote transition. Increasing evidence indicates that multiple mechanisms, involving RBPs (Zhao et al ., 2020, 2022; Dumdie et al ., 2018; Sha et al ., 2018), posttranscriptional modifications (Chang et al ., 2018; Morgan et al ., 2017; Ivanova et al ., 2017; Zhao et al ., 2017), and small RNAs (Yang et al ., 2019; Roovers et al ., 2015; Liu et al ., 2017 a ; Stein et al ., 2015), account for maternal mRNA stabilization and destabilization during oocyte maturation. Despite these exciting findings, a comprehensive understanding of the regulation of mRNA fate during oocyte maturation is yet to be established.…”

Section: Introductionmentioning

confidence: 99%

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Spatiotemporal regulation of maternalmRNAsduring vertebrate oocyte meiotic maturation

Jiang

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et al. 2023

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Vertebrate oocytes face a particular challenge concerning the regulation of gene expression during meiotic maturation. Global transcription becomes quiescent in fully grown oocytes, remains halted throughout maturation and fertilization, and only resumes upon embryonic genome activation. Hence, the oocyte meiotic maturation process is largely regulated by protein synthesis from pre‐existing maternal messenger RNAs (mRNAs) that are transcribed and stored during oocyte growth. Rapidly developing genome‐wide techniques have greatly expanded our insights into the global translation changes and possible regulatory mechanisms during oocyte maturation. The storage, translation, and processing of maternal mRNAs are thought to be regulated by factors interacting with elements in the mRNA molecules. Additionally, posttranscriptional modifications of mRNAs, such as methylation and uridylation, have recently been demonstrated to play crucial roles in maternal mRNA destabilization. However, a comprehensive understanding of the machineries that regulate maternal mRNA fate during oocyte maturation is still lacking. In particular, how the transcripts of important cell cycle components are stabilized, recruited at the appropriate time for translation, and eliminated to modulate oocyte meiotic progression remains unclear. A better understanding of these mechanisms will provide invaluable insights for the preconditions of developmental competence acquisition, with important implications for the treatment of infertility. This review discusses how the storage, localization, translation, and processing of oocyte mRNAs are regulated, and how these contribute to oocyte maturation progression.

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KnowledgeNavigator: leveraging large language models for enhanced reasoning over knowledge graph

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Wang

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Complex Intell. Syst.

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Large language models have achieved outstanding performance on various downstream tasks with their advanced understanding of natural language and zero-shot capability. However, they struggle with knowledge constraints, particularly in tasks requiring complex reasoning or extended logical sequences. These limitations can affect their performance in question answering by leading to inaccuracies and hallucinations. This paper proposes a novel framework called KnowledgeNavigator that leverages large language models on knowledge graphs to achieve accurate and interpretable multi-hop reasoning. Especially with an analysis-retrieval-reasoning process, KnowledgeNavigator searches the optimal path iteratively to retrieve external knowledge and guide the reasoning to reliable answers. KnowledgeNavigator treats knowledge graphs and large language models as flexible components that can be switched between different tasks without additional costs. Experiments on three benchmarks demonstrate that KnowledgeNavigator significantly improves the performance of large language models in question answering and outperforms all large language models-based baselines.

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Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes

Cited by 9 publications

References 42 publications

Harnessing host–virus evolution in antiviral therapy and immunotherapy

Harnessing host–virus evolution in antiviral therapy and immunotherapy

Spatiotemporal regulation of maternalmRNAsduring vertebrate oocyte meiotic maturation

KnowledgeNavigator: leveraging large language models for enhanced reasoning over knowledge graph

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