Endocan Blockade Suppresses Experimental Ocular Neovascularization in Mice

Abstract

The current data suggest that endocan is a potential therapeutic or an additional target for retinal and subretinal NV diseases.

Overexpression of miR‐181a‐5p inhibits retinal neovascularization through endocan and the ERK1/2 signaling pathway

“…Endocan mRNA levels were measured using real‐time qPCR and were also increased in the OIR retina compared with controls (Figure 1e). These results are consistent with our previous study (Su et al, 2018) and suggest that endocan may play a critical role in retinal angiogenesis.…”

“…OIR was induced as previously described (Connor et al, 2009; Su et al, 2018). Briefly, postnatal Day 7 (P7) C57BL/6J mice were exposed to 75% oxygen with their nursing mother for 5 days and returned to normal air (~21% oxygen) at P12 to receive appropriate treatments.…”

“…Mice received an intravitreal injection of miR‐181a‐5p agomir or agomir control miRNA (1 nM; RiboBio) according to the manufacturer's instructions, or with 1 μl mouse endocan neutralizing antibody (NAb; R&D Systems, Minneapolis, MN) at a concentration of 0.5 μg/μl or immunoglobulin G (IgG) isotype Ab, as previously described (Su et al, 2018). After returning to normal air at P12, mice were injected with 1 μl phosphate‐buffered saline (PBS), miR‐NC or miR‐181a‐5p mimic oligonucleotides, endocan Ab, and IgG isotype Ab ( n = 6 per group).…”

Blockade of Adenosine A2A Receptor Protects Photoreceptors after Retinal Detachment by Inhibiting Inflammation and Oxidative Stress

“…Experimental Animals. We followed the methods of Su et al [33]. All animal experiments followed the guidelines of the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by Shanghai Jiao Tong University School of Medicine Animal Care and Use Committee.…”

“…Immunofluorescence of Retinal Sections. Immunofluorescence of retinal sections was performed as previously reported [33,37]. The eyecups were cut into 10 μm thick sections.…”