Endocytic and transcytic pathways in Caco-2 cells

Modeling Kinetics of Subcellular Disposition of Chemicals

“…1658 The differentiated monolayer imitates intestinal epithelium and is widely used to assess the oral absorption potential of chemicals. [1659][1660][1661][1662][1663][1664][1665][1666][1667][1668][1669][1670][1671] The unstirred aqueous layers affect permeation through the Caco-2 monolayer. 1672 In several studies, the Caco-2 permeability was shown to be a predictor of intestinal absorption.…”

“…Initial studies, focusing on the uptake of physiologic compounds, , were soon extended to the transport setup utilizing the porous supports . The differentiated monolayer imitates intestinal epithelium and is widely used to assess the oral absorption potential of chemicals. – The unstirred aqueous layers affect permeation through the Caco-2 monolayer …”

“…Depending on the structure of transported chemicals, the Caco-2 monolayer permeation is affected by any or all of the following processes: passive transcellular transport, , active transport , and efflux, transport through tight junctions, ,, endocytosis, transcytosis, and metabolism. , This variety, although providing the opportunity to study any of the involved mechanisms, complicates the correlation of the permeability data with physicochemical properties of transported chemicals. Several attempts have been made to discern the fluxes through individual routes. – ,,,, …”

Differences in Medium-Induced Conformational Plasticity Presumably Underlie Different Cytotoxic Activity of Ricin and Viscumin

“…According to the MTS results, a one-hour treatment is sufficient to cause HT29 cell death at all concentrations used for ricin (10 −9 M, 10 −8 M, and 10 −7 M). However, in the case of viscumin, notable cell death was achieved only for the concentration of 10 −7 M. The saturation of RIP binding sites on the cell surface was achieved at a concentration of 10 −8 M [ 33 ]. Thus, the number of RIP molecules entering the cell might be significantly lower at 10 −9 M in comparison with the other two concentrations.…”