2024
DOI: 10.1002/chem.202403160
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Endogenous Cu(II) Labeling for Distance Measurements on Proteins by EPR

Hannah R. Hunter,
Shashank Kankati,
Zikri Hasanbasri
et al.

Abstract: In‐cell measurements of the relationship between structure and dynamics to protein function is at the forefront of biophysics. Recently, developments in EPR methodology have demonstrated the sensitivity and power of this method to measure structural constraints in‐cell. However, the need to spin label proteins ex‐situ or use noncanonical amino acids to achieve endogenous labeling remains a bottleneck. In this work we expand the methodology to endogenously spin label proteins with Cu(II) spin labels and describ… Show more

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