Endogenous hyaluronan in the normal and traumatized rabbit iris

Abstract

ABSTRACT.Purpose: Hyaluronan concentration in the iris tissue after iridectomy was studied in order to establish the influence of trauma on the production of hyaluronan in the anterior chamber of the eye and whether the iris participates in this process or not.

Synthesis of hyaluronan by normal and wounded rabbit iris

“…The concentration increased four-to fivefold after a peripheral iridectomy, reaching a peak of 115 µg/g on day 2 [7]. The change was statistically significant.…”

“…There is previous evidence that a larger trauma results in a greater increase in hyaluronan production than a small trauma. Complete extracapsular lens extraction resulted in more trauma than peripheral iridectomy and subsequent aqueous hyaluronan concentrations were 6.16 µg/ml and 2.0 µg/ml, respectively [7,13,14].…”

“…Endogenous hyaluronan has in the normal eye been found in the ciliary body, the aqueous humor, the trabecular meshwork, the sclera, the corneal endothelium, the zonules and the iris [7,10,12,16]. It has been shown that endogenous hyaluronan levels increase in cornea, iris and aqueous humor after trauma; extracapsular lens extraction [13], radial keratotomy [14], iridectomy [7], alkali burns [3], and excimer laser treatment [4].…”

“…It has been shown that endogenous hyaluronan levels increase in cornea, iris and aqueous humor after trauma; extracapsular lens extraction [13], radial keratotomy [14], iridectomy [7], alkali burns [3], and excimer laser treatment [4]. In the cornea it appears to be synthesized locally, but the origin of aqueous hyaluronan both in normal and wounded eyes is unknown.…”

“…However, in a previous study we could show that the hyaluronan concentration in the iris increases rapidly after peripheral iridectomy with a peak concentration on day 2, which perhaps could be the result of local synthesis in the iris. Aqueous humor hyaluronan concentrations follow the changes in the iris stroma [7].…”