Endogenous peroxynitrite activated fluorescent probe for revealing anti‐tuberculosis drug induced hepatotoxicity

Wang, Nannan; Wang, Han; Zhang, Jian; Ji, Xin; Su, Huihui; Liu, Jinying; Wang, Jiamin; Zhao, Weili

doi:10.1016/j.cclet.2021.09.046

Cited by 59 publications

(24 citation statements)

References 46 publications

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“…These herbs were shortlisted, based on the basis of their ethnopharmacological activities. Previous investigations have been performed to analyze the phytochemical profile of these herbs through analytical methods, such as FT-IR, NMR spectroscopy, and HPLC-DAD [ 14 , 28 , 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Yousuf

Shabir²,

Kauts³

et al. 2022

Molecules

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Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies.

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Section: Discussionmentioning

confidence: 99%

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Yousuf

Shabir²,

Kauts³

et al. 2022

Molecules

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“…Among the reported methods for detecting H 2 S, the fluorescence-based assay has attracted public attention because of its real-time detection, simplicity of implementation, and nondestructive analysis, − and the fluorescent method has been applied in various areas such as food examination, biological imaging, and environmental monitoring. − For instance, Yan’s group reported a turn-on fluorescent probe which could target mitochondria, image H 2 S in living cells, and judge the H 2 S level in real water samples and beer; Xiao et al developed a color and fluorescence turn-on probe for monitoring the spoilage of eggs and fish; and Wang et al constructed a ratiometric and mitochondria-targeted probe, which not only could image endogenous and exogenous H 2 S in HeLa cells but also could measure the freshness of raw meat on-site . Although several fluorescent probes have been reported for detecting H 2 S, however, the majority of them is unable to visualize H 2 S in vivo primarily on account of their short emission wavelength (<650 nm), and only a few of the reported probes could detect H 2 S gas.…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Hydrogen Sulfide in Food Spoilage, Living Cells, and Zebrafish

Yang

Wang

et al. 2022

J. Agric. Food Chem.

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Hydrogen sulfide (H2S) is a significant component of various physiological processes, and it can also cause a negative effect on foodstuffs. In this work, we designed and synthesized an NIR fluorescent turn-on responding probe (DDM-H 2 S) with a large Stokes shift (190 nm) for the detection of H2S. DDM-H 2 S exhibited high selectivity and sensitivity, obvious color changes, and a fast response time for tracing H2S. When DDM-H 2 S reacted with H2S, the PET process was eliminated, and the recovered ICT process and NIR fluorescence were observed. Moreover, DDM-H 2 S could image endogenous and exogenous H2S in living HeLa cells and zebrafish. What is more, the probe DDM-H 2 S could be deposited easily to test paper strips, which were able to detect the H2S gas produced during food spoilage (such as eggs, raw meat, and fishes) by the color of test paper strips changing from pink to purple. Therefore, this work provides a promising approach for monitoring H2S in complicated biological systems and practical food samples.

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“…The selection of HClO-sensitive functional groups includes pmethoxyphenol, [12] hydroxylamine [13] and chalcogenide. [14] Among them, the fluorescent probes with aldoxime group conjugated with BODIPY, [15][16][17][18][19][20] rhodamine, [21] and phenanthroline [22] have exhibited great response. In particular, the aldoxime group can react with HClO to generate aldehyde, carboxylic acid, or nitrile oxide, which leads to the structure convert oriented emission change.…”

Section: Introductionmentioning

confidence: 99%

A BODIPY‐Based Far‐Red‐Absorbing Fluorescent Probe for Hypochlorous Acid Imaging

Wan

Wang

et al. 2022

ChemPhotoChem

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Hypochlorous acid (HClO) is produced by white blood cells to defend against injury and bacteria. However, as one of the reactive oxygen species, high intracellular HClO concentration could lead to chronic diseases that affect the cardiovascular and nervous systems. To monitor HClO concentrations in bio‐samples, the fluorescent probe is preferred to have: a) Absorbability in the far‐red window with reduced light toxicity and improved tissue penetration depth; b) Ratiometric features for accurate analysis. In this study, we reported a far‐red ratiometric HClO fluorescence probe based on BODIPY chromophore and aldoxime sensing group. Not only the color change of the probe solution can be detected by the naked eye, but also the emission ratios (I645/I670) showed a significant increase upon the introduction of HClO. More importantly, the feasibility of HClO monitoring in biosamples was demonstrated in vitro using a confocal microscope.

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Endogenous peroxynitrite activated fluorescent probe for revealing anti‐tuberculosis drug induced hepatotoxicity

Cited by 59 publications

References 46 publications

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Hydrogen Sulfide in Food Spoilage, Living Cells, and Zebrafish

A BODIPY‐Based Far‐Red‐Absorbing Fluorescent Probe for Hypochlorous Acid Imaging

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