Endogenous synthesis of n−3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse

Kelton, D.F.; Lysecki, Clara; Aukema, Harold M.; Anderson, Breanne M.; Kang, Jing; Ma, Dávid

doi:10.1016/j.plefa.2013.08.002

Cited by 7 publications

(10 citation statements)

References 56 publications

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“…Mice that received herring-derived omega-3 phospholipids concentrate rich in phosphatidylcholine (10 % w/w) had elevated levels of n-3 phosphatidylcholine and phosphatidylethanolamine in the liver [52]. This also occurs with fat-1 mice (endogenous production of n-3 PUFA), which have elevated n-3 containing phospholipids in the kidney [53]. In healthy subjects, fish oil supplementation alters lipid metabolism and increases the proportion of phospholipids and triacylglycerol containing long-chain polyunsaturated fatty acids.…”

Section: Discussionmentioning

confidence: 95%

Effects of a Diet Rich in n‐3 Polyunsaturated Fatty Acids on Hepatic Lipogenesis and Beta‐Oxidation in Mice

Bargut

Frantz

Mandarim-de-Lacerda

et al. 2014

Lipids

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Here, we investigate whether a diet rich in fish oil can lead to the development of hepatic alterations associated with non-alcoholic fatty liver disease (NAFLD). To achieve this goal, we provided, for 8 weeks, four different diets to 3-month-old C57BL/6 mice: (a) standard-chow diet (SC; 40 g soybean oil/kg diet, 10 % of the total energy content from lipids), (b) fish oil diet (FO; 4 g soybean oil and 36 g fish oil/kg diet, 10 % of the total energy content from lipids), (c) high-fat diet (HF; 40 g soybean oil and 238 g lard/kg diet, 50 % of the total energy content from lipids), and (d) high-fish oil diet (HFO; 40 g soybean oil and 238 g fish oil/kg diet, 50 % of the total energy content from lipids). Biochemical analyses, stereology, western-blotting and RT-qPCR were used. In the HF group, we found evidence of obesity, metabolic syndrome, and liver damage, along with hypertriglyceridemia, hepatic insulin resistance, and steatosis. On the other hand, the HFO group did not present these alterations and remained similar to the controls. The changes observed in the animals fed the HF diet were accompanied by an increase in hepatic lipogenesis and a decrease in beta-oxidation; meanwhile, in the HFO group, the opposite results were found, that is, reduced lipogenesis and elevated beta-oxidation, were most likely responsible for the prevention of deleterious hepatic alterations and liver damage. In conclusion, a diet rich in fish oil has beneficial effects on hepatic insulin resistance, lipogenesis and beta-oxidation and prevents hepatic tissue from liver damage and NAFLD.

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Section: Discussionmentioning

confidence: 95%

Effects of a Diet Rich in n‐3 Polyunsaturated Fatty Acids on Hepatic Lipogenesis and Beta‐Oxidation in Mice

Bargut

Frantz

Mandarim-de-Lacerda

et al. 2014

Lipids

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“…This occurred in part by the increased FAT / CD36 gene expression, which, along with other genes, is able to regulate the homeostasis of free fatty acids (Kelton et al . ). Free fatty acids released by circulating lipoproteins are rapidly absorbed through the FAT/CD36 membrane receptor by adipocytes.…”

Section: Discussionmentioning

confidence: 97%

Enhanced pan‐peroxisome proliferator‐activated receptor gene and protein expression in adipose tissue of diet‐induced obese mice treated with telmisartan

Penna-de-Carvalho

Graus-Nunes

Rabelo-Andrade

et al. 2014

Experimental Physiology

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New Findings r What is the central question of this study?Telmisartan, an antihypertensive, has beneficial side-effects through its peroxisome proliferator-activated receptor (PPAR) β/δ agonism in white adipose tissue, besides its well-known property of partial PPARγ agonism. Here, we investigated a potential pan-PPAR role of this drug in the white and brown adipose tissues. r What is the main finding and its importance? A. Penna-de-Carvalho and others telmisartan ameliorates inflammation and insulin resistance, as well as inducing non-shivering thermogenesis. Our results point to new therapeutic targets for the control of obesity and comorbidities through pan-PPAR-related effects.

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“…Almost no data is available on the differences in fatty acids and oxylipins in fat-1 versus WT mice after dietary supplementation with n3-PUFA. The only available study compares the effects of nine weeks of feeding on selected oxylipins and fatty acids in kidney tissue [ 39 ]. Therefore, in the present study we thoroughly investigated the modulation of both, the total fatty acid and oxylipin profile in fat-1 vs WT mice on a standard, sunflower oil based diet and the same diet enriched with n3-PUFA (1% EPA and 1% DHA).…”

Section: Introductionmentioning

confidence: 99%

Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo—A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet

et al. 2017

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Dietary intervention and genetic fat-1 mice are two models for the investigation of effects associated with omega-3 polyunsaturated fatty acids (n3-PUFA). In order to assess their power to modulate the fatty acid and oxylipin pattern, we thoroughly compared fat-1 and wild-type C57BL/6 mice on a sunflower oil diet with wild-type mice on the same diet enriched with 1% EPA and 1% DHA for 0, 7, 14, 30 and 45 days. Feeding led after 14–30 days to a high steady state of n3-PUFA in all tissues at the expense of n6-PUFAs. Levels of n3-PUFA achieved by feeding were higher compared to fat-1 mice, particularly for EPA (max. 1.7% in whole blood of fat-1 vs. 7.8% following feeding). Changes in PUFAs were reflected in most oxylipins in plasma, brain and colon: Compared to wild-type mice on a standard diet, arachidonic acid metabolites were overall decreased while EPA and DHA oxylipins increased with feeding more than in fat-1 mice. In plasma of n3-PUFA fed animals, EPA and DHA metabolites from the lipoxygenase and cytochrome P450 pathways dominated over ARA derived counterparts.Fat-1 mice show n3-PUFA level which can be reached by dietary interventions, supporting the applicability of this model in n3-PUFA research. However, for specific questions, e.g. the role of EPA derived mediators or concentration dependent effects of (individual) PUFA, feeding studies are necessary.

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Endogenous synthesis of n−3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse

Cited by 7 publications

References 56 publications

Effects of a Diet Rich in n‐3 Polyunsaturated Fatty Acids on Hepatic Lipogenesis and Beta‐Oxidation in Mice

Effects of a Diet Rich in n‐3 Polyunsaturated Fatty Acids on Hepatic Lipogenesis and Beta‐Oxidation in Mice

Enhanced pan‐peroxisome proliferator‐activated receptor gene and protein expression in adipose tissue of diet‐induced obese mice treated with telmisartan

Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo—A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet

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