Endolymphatic hydrops induced by chronic administration of vasopressin

Takeda, Taizo; Takeda, Setsuko; Kitano, Hiroya; Okada, Teruhiko; Kakigi, Akinobu

doi:10.1016/s0378-5955(99)00180-x

Cited by 134 publications

(124 citation statements)

References 16 publications

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“…39 Morphologically, the endolymphatic space was found to increase in response to systemic application of VP. 13 Clinically, Takeda et al 9 demonstrated evidence of elevated serum arginine VP levels in patients with MD. Hornibrool et al 40 reported that there have not been abnormal plasma levels of arginine VP in patients of MD.…”

Section: Discussionmentioning

confidence: 99%

“…8 Recently, it has come to light that this mechanism has a crucial role not only in the kidney but also in the inner ear. In addition, the following evidence has accumulated to suggest that VP is closely associated with the formation of EH: (1) plasma levels of arginine VP are higher in patients with MD and may depend on the phase that the patient is in, [9][10][11] (2) acute and chronic application of arginine VP produces EH in guinea-pigs and rats, [12][13][14] (3) V2 receptor mRNA is expressed in rat and human inner ear, [15][16][17][18] and (4) expression of V2 receptor mRNA in the rat inner ear is downregulated by VP application. 19 Such accumulated evidence has led to the assumption that production of endolymph is controlled by VP-AQP2 system.…”

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confidence: 99%

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Morphological and functional changes in a new animal model of Ménière's disease

Egami

Kakigi

Sakamoto

et al. 2013

Laboratory Investigation

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The purpose of this study was to clarify the underlying mechanism of vertiginous attacks in Ménière's disease (MD) while obtaining insight into water homeostasis in the inner ear using a new animal model. We conducted both histopathological and functional assessment of the vestibular system in the guinea-pig. In the first experiment, all animals were maintained 1 or 4 weeks after electrocauterization of the endolymphatic sac of the left ear and were given either saline or desmopressin (vasopressin type 2 receptor agonist). The temporal bones from both ears were harvested and the extent of endolymphatic hydrops was quantitatively assessed. In the second experiment, either 1 or 4 weeks after surgery, animals were assessed for balance disorders and nystagmus after the administration of saline or desmopressin. In the first experiment, the proportion of endolymphatic space in the cochlea and the saccule was significantly greater in ears that survived for 4 weeks after surgery and were given desmopressin compared with other groups. In the second experiment, all animals that underwent surgery and were given desmopressin showed spontaneous nystagmus and balance disorder, whereas all animals that had surgery but without desmopressin administration were asymptomatic. Our animal model induced severe endolymphatic hydrops in the cochlea and the saccule, and showed episodes of balance disorder along with spontaneous nystagmus. These findings suggest that administration of desmopressin can exacerbate endolymphatic hydrops because of acute V2 (vasopressin type 2 receptor)-mediated effects, and, when combined with endolymphathic sac dysfunction, can cause temporary vestibular abnormalities that are similar to the vertiginous attacks in patients with MD. Ménière's disease (MD) is a well-known inner ear disorder characterized by symptoms including recurring attacks of vertigo typically lasting for hours, fluctuating sensorineural hearing loss, and tinnitus. Since the milestone findings on the temporal bones of MD patients, 1,2 endolymphatic hydrops (EH) has been considered as the histopathological origin of MD, as characteristic morphological changes were reported to be produced by surgical obstruction of the endolymphatic sac (ES) in guinea-pig. 3 This observation indicates that malabsorption of endolymph in the ES is one of the possible mechanisms underlying the development of EH. This experimentally induced EH has been frequently used as an animal model to investigate the pathogenesis of inner ear disorders associated with EH. In terms of vestibular function, while canal dysfunction and/or spontaneous nystagmus have been occasionally observed in this animal model, 4,5 episodes of vertiginous attack or balance disorder have only rarely been observed. 6 In other words, this model is insufficient for fully representing the common symptoms of MD.Water homeostasis of the inner ear is essential for maintaining the functions of hearing and balance. EH is considered to be the result of disruption of inner ear water homeostasis, which ...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Morphological and functional changes in a new animal model of Ménière's disease

Egami

Kakigi

Sakamoto

et al. 2013

Laboratory Investigation

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“…These findings also suggest that vasopressin directly regulates fluid levels in the inner ear. We performed almost the same experiments in guinea pigs [14]. The dosage of AVP in the present study was 8 mU/kg/min and the dosages in the guinea pigs were from 0.2 to 1 mU/kg/min.…”

Section: Discussionmentioning

confidence: 89%

“…The increase in the endolymphatic space was quantitatively analyzed in the same way as reported by Takeda et al [14].…”

Section: Histological Examination Of Rat Inner Earsmentioning

confidence: 98%

Endolymphatic Hydrops by Administration of Vasopressin in the Rat.

Kitano

Takeda²,

Takeda³

et al. 2001

Acta Histochem. Cytochem

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The anti-diuretic hormone, vasopressin, has been shown to be important in regulating inner ear fluid. We report on the involvement between Meniere's disease and vasopressin. We designed an experiment to determine whether vasopressin directly affects the fluid level. We chronically infused this hormone in rats and examined them for morphological signs of endolymphatic hydrops. Vasopressin infusion significantly increased the scala media area by 5.9r7.1% (P0.01), and the length of Reissner's membrane by 5.9r3.2% (P0.001). The results suggest that high plasma levels of vasopressin may be a principal causal factor of endolymphatic hydrops in Meniere's disease, perhaps due to down-regulating the number of vasopressin receptors.

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“…In fact, the stria vascularis (SV) plays an important role in the inner fluid homeostasis [25]. Experimentally, the administration of vasopressin was reported to induce endolymphatic hydrops [10,20], and vasopressin type 2 receptor was demonstrated in the SV (unpublished data). These reports support the hypothesis that SV regulates the volume of cochlear fluid and generates endolymph via hormones.…”

Section: Introductionmentioning

confidence: 98%