Endometrial cancer and its cell lines

Skok, Kristijan; Maver, Uroš; Gradišnik, Lidija; Kozar, Nejc; Takač, Iztok; Arko, Darja

doi:10.1007/s11033-019-05226-3

Cited by 17 publications

(23 citation statements)

References 57 publications

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“…Among the studies that tackle the issue of EC CLs some of them show similarities while others differ in certain aspects 8,9,50,51,53 . Our findings from the literature were that HEC‐1‐A, HEC‐1‐B and KLE are reported differently 2 . Crucial properties and the originators' information of the most common EC CLs are succinctly summarized in Table 5.…”

Section: Cancer Of the Endometrium And Its Cell Linesmentioning

confidence: 72%

“…Other issues include the misidentification and redundancy of these cell isolates. This was shown by Korch et al in 2012, 50 who presented proof that ECC‐1 isolates were contaminated and genotyped either as Ishikawa cells, MCF‐7 breast cancer cells (or a combination) 2 . Furthermore, another issue is the uncertainty regarding their primary type (I or II) due to conflicting reports in the literature 8,51,52 .…”

Section: Cancer Of the Endometrium And Its Cell Linesmentioning

confidence: 96%

“…Culturing procedures have drastically changed over the years, especially with the development of new reagents and new materials. If comparing our current procedures with the past, we can now appreciate a much more streamlined process 2 . The core principle, which is the usage of a culturing medium that supports the growth of cells as well as additions in the form of antibiotics that stop the growth of bacteria, is of course universally the same.…”

Section: Cell Line Culturingmentioning

confidence: 99%

“…One of the oldest and still most recurring problems in CL culturing remains to be the potential of genetic or epigenetic changes can potentially arise during their growth and use. The latter limits the correlation potential of results based on these with the properties of the primary tissue 2,4,5 . By this, also their clinical relevance might be questioned.…”

Section: Introductionmentioning

confidence: 99%

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Gynaecological cancers and their cell lines

Skok

Gradišnik

Maver

et al. 2021

J Cellular Molecular Medi

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Cell lines are widely used for various research purposes including cancer and drug research. Recently, there have been studies that pointed to discrepancies in the literature and usage of cell lines. That is why we have prepared a comprehensive overview of the most common gynaecological cancer cell lines, their literature, a list of currently available cell lines, and new findings compared with the original studies. A literature review was conducted via MEDLINE, PubMed and ScienceDirect for reviews in the last 5 years to identify research and other studies related to gynaecological cancer cell lines. We present an overview of the current literature with reference to the original studies and pointed to certain inconsistencies in the literature. The adherence to culturing rulesets and the international guidelines helps in minimizing replication failure between institutions. Evidence from the latest research suggests that despite certain drawbacks, variations of cancer cell lines can also be useful in regard to a more diverse genomic landscape.

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Section: Cancer Of the Endometrium And Its Cell Linesmentioning

confidence: 72%

Section: Cancer Of the Endometrium And Its Cell Linesmentioning

confidence: 96%

Section: Cell Line Culturingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gynaecological cancers and their cell lines

Skok

Gradišnik

Maver

et al. 2021

J Cellular Molecular Medi

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“…In addition, GTEE increased the expression of cyclins B and E in HEC‐1‐A cells, but not in KLE and AN3 CA cells (Figure 2C). However, the doubling times of HEC‐1‐A, KLE, and AN3 CA cells were found to be 27 to 31 hours, 45 to 55 hours and 34 to 114 hours, respectively 19 . Therefore, after GTEE treatment, the increased expression of cyclins B and E may cause a shorter doubling time in HEC‐1‐A cells.…”

Section: Discussionmentioning

confidence: 96%

Ganoderma tsugae suppresses the proliferation of endometrial carcinoma cells via Akt signaling pathway

Tsai

Kuo

et al. 2020

Environmental Toxicology

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Ganoderma is one of the common medicinal mushrooms in traditional Chinese medicine. Previous researches have unveiled the multifaceted biological activity of Ganoderma extract. Ganoderma tsugae has been investigated the potential on curing prostate, colon, lung, epidermoid, breast and ovarian cancers, but not including endometrial cancer. Endometrial cancer is a gynecological malignant tumor with serious drug resistance problem in clinical cancer treatment. This study aimed to demonstrate the first study of Ganoderma on treating endometrial cancer. The Ganoderma tsugae ethanol extract (GTEE) could suppress the proliferation of endometrial cancer cells HEC‐1‐A, KLE, and AN3 CA. GTEE also induced G1/S phase arrest and mitochondria‐mediated apoptosis in endometrial cancer cells. Furthermore, the Akt signaling pathway could be suppressed by GTEE. Therefore, our results suggest for the first time that GTEE has the potential to be an adjuvant therapeutic agent in the treatment of endometrial cancer.

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A New Type of Endometrial Cancer Models in Mice Revealing the Functional Roles of Genetic Drivers and Exploring their Susceptibilities

Chen,

Dai,

Zhao

et al. 2023

Advanced Science

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Endometrial cancer (EC) is the most common female reproductive tract cancer and its incidence has been continuously increasing in recent years. The underlying mechanisms of EC tumorigenesis remain unclear, and efficient target therapies are lacking, for both of which feasible endometrial cancer animal models are essential but currently limited. Here, an organoid and genome editing‐based strategy to generate primary, orthotopic, and driver‐defined ECs in mice is reported. These models faithfully recapitulate the molecular and pathohistological characteristics of human diseases. The authors names these models and similar models for other cancers as organoid‐initiated precision cancer models (OPCMs). Importantly, this approach can conveniently introduce any driver mutation or a combination of driver mutations. Using these models,it is shown that the mutations in Pik3ca and Pik3r1 cooperate with Pten loss to promote endometrial adenocarcinoma in mice. In contrast, the Kras G12D mutati led to endometrial squamous cell carcinoma. Then, tumor organoids are derived from these mouse EC models and performed high‐throughput drug screening and validation. The results reveal distinct vulnerabilities of ECs with different mutations. Taken together, this study develops a multiplexing approach to model EC in mice and demonstrates its value for understanding the pathology of and exploring the potential treatments for this malignancy.

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Endometrial cancer and its cell lines

Cited by 17 publications

References 57 publications

Gynaecological cancers and their cell lines

Gynaecological cancers and their cell lines

Ganoderma tsugae suppresses the proliferation of endometrial carcinoma cells via Akt signaling pathway

A New Type of Endometrial Cancer Models in Mice Revealing the Functional Roles of Genetic Drivers and Exploring their Susceptibilities

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