Endometrial flushing α_Vβ₃ integrin, glycodelin and PGF2α levels for evaluating endometrial receptivity in women with polycystic ovary syndrome, myoma uteri and endometrioma

Abstract

The aim of this cross-sectional study is to compare endometrial flushing fluid levels of αβ integrin, glycodelin and PGF2α during the midluteal phase of the menstrual cycle of women with polycystic ovary syndrome (PCOS, n = 20), myoma uteri (n = 20) and endometrioma (n = 19) with the healthy controls (n = 20). After collecting samples at the midluteal phase of ovulatory volunteers and storing them at -80 °C, αβ integrin, glycodelin and PGF2α levels were analyzed using ELISA. The mean ages of the groups were 28.90 ± 5.45, 37.25 ± 2.73, 32.84 ± 6.62 and 32.15 ± 5.18 in PCOS, myoma uteri, endometrioma and control groups, respectively. The αβ integrin level (ng/ml) was statistically significantly higher in endometrioma group (9.70 ± 1.72, p < 0.05) as compared to myoma uteri and control groups. Similarly, glycodelin level (ng/ml) was significantly higher in endometrioma group (341.04 ± 93.32) than PCOS (p < 0.01), myoma uteri (p < 0.001) and healthy subjects (p < 0.001). Moreover, PGF2α level (350.04 ± 464.50 ng/ml) was significantly higher in PCOS group relative to myoma uteri (p < 0.001), endometrioma (p < 0.05) and control (p < 0.05) groups. In conclusion, αβ integrin level was significantly higher in endometrioma subjects than those with myoma uteri and control groups; glycodelin level was significantly higher in endometrioma group than other three groups, and lastly, PCOS patients had significantly higher PGF2α levels than those patients with myoma uteri, endometrioma and controls.

Endometrial Flushing Tumor Necrosis Factor Alpha and Interleukin 2 Levels in Women with Polycystic Ovary Syndrome, Leiomyoma and Endometrioma: Comparison with Healthy Controls

“…That study included structural proteins and progesterone-regulated molecules. This previous study found that αVβ3 integrin and glycodelin levels were higher in the endometrioma and PGF2α was higher in the PCOS patients than the control group in a statistically significant way [12].…”

“…In our 2017 study, we had chosen to evaluate three cell signaling molecules that have impact on endometrial receptivity with mostly paracrine effect [12]. That study included structural proteins and progesterone-regulated molecules.…”

“…All subjects were informed in detail about the research project verbally and their written informed consent was obtained. The study was conducted with patients that were also part of another study [12]. This other study focused on the levels of structural proteins and progesterone regulated molecules (αVβ3 integrin, glycodelin and PGF2α).…”

Combined effect of dydrogesterone and letrozole on humoral immune function, sex hormone levels and serology-related indices in patients with endometriosis

“…Pre-and posttreatment levels of IgA, FSH and LH did not differ appreciably between the two groups, but posttreatment E2 was significantly lower in the study group. By mediating immune-inflammatory reactions, IL-6 causes local pelvic fibrosis and adhesion, and aggravates cytotoxicity, thereby promoting the development of clinical symptoms seen in endometrial women [13]. Studies have shown that TNF-α is associated with dysregulation of cytokines, and is highly expressed in endometriosis [14].…”

Oxidative Stress in Endometrial Flushing Fluid of Patients with Polycystic Ovary Syndrome, Endometrioma and Uterine Leiomyoma: Comparison with Healthy Controls

“…The Helsinki declaration was followed. The study was conducted with patients that were also part of another study (13). The current study focuses on the comparison of OS markers among healthy patients and patients with benign gynecological diseases, whereas the earlier study investigated endometrial receptivity marker levels of the same population.…”