Endometrial reconstruction from stem cells

Gargett, Caroline E.; Ye, Louie

doi:10.1016/j.fertnstert.2012.05.004

Cited by 160 publications

(122 citation statements)

References 79 publications

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“…Several studies (28)(29)(30) have reported that EnSCs may contribute to endometrial repair. Low proliferation and differentiation rate of EnSCs may lead to a variety of endometrial diseases, including endometriosis, endometrial polyps or even endometrial cancer (31).…”

Section: Discussionmentioning

confidence: 99%

Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury

et al. 2018

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Abstract. The present study investigated the effects of endometrial stem cell (EnSCs) transplantation combined with estrogen in the repair of endometrial injury. A total of 30 patients with intrauterine adhesions (IUA) and 30 healthy individuals were selected. Expression of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-BB in endometrial tissue was assessed. Additionally, expression levels of epithelial membrane antigen (EMA), cytokeratin (CK), integrin α-6 (CD49f), Thy-1 membrane glycoprotein (THY-1), collagen type 1 (Col I), fibroblast (5B5) and vimentin in EnSCs were detected using western blot analysis and reverse transcription-quantitative polymerase chain reaction. A rat model of IUA was established and female rats were divided into the control, model, EnSCs, estrogen and estrogen plus EnSCs (E+EnSCs) groups. Blood was extracted at 1 and 5 weeks post-treatment, and serum levels of transforming growth factor (TGF)-β1, EGF, 17 β-estradiol (E2) and PDGF-BB were measured using ELISA. Hematoxylin and eosin staining was performed to observe the pathological changes of endometrial tissue in rats. Western blot analysis was used to detect the expression of estrogen receptor (ESR1) and matrix metalloproteinase (MMP)-9 in the endometrium. The results revealed that patients with IUA exhibited increased expression levels of EGF and PDGF-BB compared with those in control group. Additionally, EnSCs exhibited significantly increased expression levels of EMA, CD49f, CK, Col I, THY-1, 5B5 and vimentin compared with the remaining groups. An increased number of newly formed glands was observed in the E+EnSCs group compared with that in the EnSCs group.Increased levels of E2, but decreased levels of TGF-β1, EGF, PDGF-BB, ESR1 and MMP-9 were detected in EnSCs and estrogen groups compared with those in E+EnSCs group. These results suggest that EnSCs transplantation combined with estrogen could improve endometrial abnormalities. IntroductionIntrauterine adhesions (IUA) refer to the endometrial fibrosis caused by endometrial injury in operative hysteroscopy (1). Endometrial injuries may lead to recurrent miscarriages, infertility, amenorrhea, abnormal placentation, dysmenorrhea and abnormal uterine bleeding, thus being detrimental to the health of affected women (2). Expression levels of transforming growth factor (TGF)-β1, epidermal growth factor (EGF), basic fibroblast growth factor and platelet-derived growth factor (PDGF)-BB were found to be significantly increased in exudates in patients with IUA following surgery (3,4), which in turn promoted the growth of fibroblasts and the formation of adhesions. Despite advances in intraoperative techniques, including minimizing trauma of the healthy myometrium and endometrium, and reducing the usage of electrosurgery (5), the incidence of IUA caused by surgery remains high. Therefore, the development of novel approaches to inhibit the formation of post-surgical IUA is required.A variety of novel therapies for IUA have been developed. Estrogen is the most importa...

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Section: Discussionmentioning

confidence: 99%

Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury

et al. 2018

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“…Self-renewing stem cells which have high proliferative and differentiative capacity would be ideal for recellularization purposes. Current attempts to reconstruct the endometrium using stem cells from different sources have been reviewed [44]. The human endometrium contains a small proportion of both stromal and epithelial progenitor cells [45].…”

Section: Discussionmentioning

confidence: 99%

Development of a novel human recellularized endometrium that responds to a 28-day hormone treatment†

Olalekan

Burdette

Getsios

et al. 2017

Biology of Reproduction

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Three-dimensional (3D) in vitro models have been established to study the physiology and pathophysiology of the endometrium. With emerging evidence that the native extracellular matrix (ECM) provides appropriate cues and growth factors essential for tissue homeostasis, we describe, a novel 3D endometrium in vitro model developed from decellularized human endometrial tissue repopulated with primary endometrial cells. Analysis of the decellularized endometrium using mass spectrometry revealed an enrichment of cell adhesion molecules, cytoskeletal proteins, and ECM proteins such as collagen IV and laminin. Primary endometrial cells within the recellularized scaffolds proliferated and remained viable for an extended period of time in vitro. In order to evaluate the hormonal response of cells within the scaffolds, the recellularized scaffolds were treated with a modified 28-day hormone regimen to mimic the human menstrual cycle. At the end of 28 days, the cells within the endometrial scaffold expressed both estrogen and progesterone receptors. In addition, decidualization markers, IGFBP-1 and prolactin, were secreted upon addition of dibutyryl cyclic AMP indicative of a decidualization response. This 3D model of the endometrium provides a new experimental tool to study endometrial biology and drug testing. Summary SentencePrimary endometrial cells within a recellularized scaffold respond to a 28-day menstrual cycle.

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“…Mesenchymal stem cells derived from bone marrow, adipose tissue and other sources have been long used for cell replacement therapies with varying degrees of success. Studies from our laboratory and others have recently concentrated on the endometrium, which contains a rich supply of mesenchymal stem cell-like cells [61]. In fact, the endometrium is the only source of tissue derived mesenchymal stem cells that can be obtained without analgesic requirements, thereby rendering it a feasible and functional source for stem cell therapies.…”

Section: Differentiation Of Endometrium-derived Stem Cells To Treat Nmentioning

confidence: 99%

The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

Mutlu

Hufnagel

Taylor

2015

Biology of Reproduction

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Stem cell therapies have opened new frontiers in medicine with the possibility of regenerating lost or damaged cells. Embryonic stem cells, induced pluripotent stem cells, hematopoietic stem cells, and mesenchymal stem cells have been used to derive mature cell types for tissue regeneration and repair. However, the endometrium has emerged as an attractive, novel source of adult stem cells that are easily accessed and demonstrate remarkable differentiation capacity. In this review, we summarize our current understanding of endometrial stem cells and their therapeutic potential in regenerative medicine.

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Endometrial reconstruction from stem cells

Cited by 160 publications

References 79 publications

Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury

Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury

Development of a novel human recellularized endometrium that responds to a 28-day hormone treatment†

The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

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