Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

Salmi, Teea; Collin, Pekka; Korponay-Szabó, Ilma Rita; Laurila, Kaija; Partanen, Jukka; Huhtala, Heini; Király, Róbert; Lóránd, L.; Reunala, Timo; Mäki, Markku; Kaukinen, Katri

doi:10.1136/gut.2005.071514

Cited by 228 publications

(190 citation statements)

References 43 publications

(36 reference statements)

Supporting

Mentioning

167

Contrasting

Unclassified

Order By: Relevance

“…Autoantibodies can be present only in the mucosa. 35 All "seronegative" relatives had sub-total VA. Thus, although a previous study demonstrated that endomysial positivity depends on severity of VA, 36 the degree of the lesion didn't explain the absence of circulating antibodies in our patients.…”

Section: Discussionmentioning

confidence: 98%

Predictors of Family Risk for Celiac Disease: A Population-Based Study

Rubio–Tapia

Dyke

Lahr

et al. 2008

Clinical Gastroenterology and Hepatology

138

130

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 98%

Predictors of Family Risk for Celiac Disease: A Population-Based Study

Rubio–Tapia

Dyke

Lahr

et al. 2008

Clinical Gastroenterology and Hepatology

138

130

View full text Add to dashboard Cite

show abstract

“…However, intestinal anti-TG2 antibodies may be even more relevant from a clinical viewpoint. These antibodies are produced at intestinal level [6], where they could be deposited even before they appear in circulation [7,20]. Two aspects can make intestinal anti-TG2 antibodies relevant: the first is their suggested ability in potential coeliac patients to predict evolution towards a clear enteropathy; the second is their possible role in revealing a condition of gluten sensitivity in patients with absence of CD-associated autoantibodies in their serum [21,22].…”

Section: Discussionmentioning

confidence: 99%

Intestinal anti-tissue transglutaminase antibodies in potential coeliac disease

Tosco

Aitoro

Auricchio

et al. 2012

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryAnti-tissue transglutaminase 2 (anti-TG2) antibodies are present in the serum of the great majority of untreated coeliac disease (CD) patients. They are produced and deposited in the small intestinal mucosa. Potential CD patients present serum anti-TG2 antibodies higher than cut-off, but a normal duodenal mucosa where mucosal deposits of anti-TG2 are not always detectable. The aim of our work was to investigate the presence of anti-TG2 intestinal antibodies in patients with potential CD, and identify the most sensitive test to detect them. Twelve active CD patients, 28 potential CD patients and 39 non-CD controls were enrolled. Biopsy fragments from all patients were analysed by double immunofluorescence to detect mucosal deposits of anti-TG2 antibodies. Fragments from the same subjects were also cultured for 24 h with medium in the presence or absence of gliadin peptides. Anti-TG2 autoantibodies secreted into supernatants were measured by enzyme-linked immunosorbent assay. All active CD, 68% of potential CD patients and 20% of non-CD controls showed mucosal deposits of immunoglobulin (Ig)A anti-TG2; at the same time 100, 96 and 8% of active CD, potential CD and non-CD control patients secreted these antibodies in culture supernatants, respectively. Our data showed that, to detect intestinal anti-TG2 antibodies, the measurement of antibodies secreted into culture supernatants has higher sensitivity and specificity (97·5 and 92·3%, respectively) than the detection of mucosal deposits (77·5 and 80·0%, respectively). The measurement of intestinal anti-TG2 antibodies may prove useful in clinical practice to predict evolution towards mucosal atrophy in potential coeliac patients and identify patients with gluten sensitivity.

show abstract

“…Glutene duyarlı enteropati, çölyak "sprue" (nontropical sprue) olarak da adlandırılır. Bir enteropati olarak bilinmesine rağmen son yıllarda ortaya konulan ve belirginleşen gastrointestinal sistem dışı bulguları ile her sistemin hastalığı haline gelmiştir (3). Çocukluk çağının en yaygın malabsorbsiyon nedeni olan bu hastalık çocukları ve eriş-kinleri yaşam boyu etkilemekte ve her yaşta ortaya çıka-bilmektedir (3).…”

Section: Introductionunclassified

“…Çölyak hastalığında glutenin içindeki gliadin veya çeşitli dokulara karşı IgA'nın ön planda olduğu güçlü humoral ve sitotoksik hücresel immün yanıt gelişir. Gliadin molekülü tümüyle toksiktir (3,4). Hastalık ince barsak (İB) mukozasında intraepitelyal lenfosit (IEL) artışı, kript hiperplazisi ve villus atrofisi bulguları ile tanı alır (5).…”

Section: Introductionunclassified