Endophilin B is required for the <i>Drosophila</i> oocyte to endocytose yolk downstream of Oskar

Tsai, Yi Cheng; Chiang, Wei; Liou, Willisa; Lee, Wei Hao; Chang, Yu Wei; Wang, Pei Yu; Li, Yi Chen; Tanaka, Tsubasa; Nakamura, Akira; Pai, Li‐Mei

doi:10.1242/dev.097022

Cited by 6 publications

(10 citation statements)

References 54 publications

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“…Both Short and Long Oskar localize at the posterior pole of oocytes. However, while Short Oskar localizes, together with Vasa, to electrondense particles termed polar granules, Long Oskar is excluded from these structures and instead localizes to endocytic membranes along the oocyte cortex (Breitwieser et al 1996;Mahowald 1962;Tsai et al 2014;Vanzo et al 2007). Unlike Short Oskar, Long Oskar is not present in pole cells (Markussen et al 1995).…”

Section: Oskar -The Drosophila Germ Plasm Organizermentioning

confidence: 99%

LOTUS-domain proteins - developmental effectors from a molecular perspective

Kubíková

Reinig

Salgania

et al. 2020

Biological Chemistry

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The LOTUS domain (also known as OST-HTH) is a highly conserved protein domain found in a variety of bacteria and eukaryotes. In animals, the LOTUS domain is present in the proteins Oskar, TDRD5/Tejas, TDRD7/TRAP/Tapas, and MARF1/Limkain B1, all of which play essential roles in animal development, in particular during oogenesis and/or spermatogenesis. This review summarizes the diverse biological as well as molecular functions of LOTUS-domain proteins and discusses their roles as helicase effectors, posttranscriptional regulators, and critical co-factors of piRNA-mediated transcript silencing.

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Section: Oskar -The Drosophila Germ Plasm Organizermentioning

confidence: 99%

LOTUS-domain proteins - developmental effectors from a molecular perspective

Kubíková

Reinig

Salgania

et al. 2020

Biological Chemistry

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“…Loss of EndoA has been shown to cause severe endocytic defects and impair neurotransmission in various models (Verstreken et al ., 2002 ; Milosevic et al ., 2011 ). Endophilin B (D‐EndoB) is required for yolk endocytosis in Drosophila melanogaster ; loss of D‐EndoB reduces yolk content similar to yolkless mutants and results in low female fertility (Tsai et al ., 2014 ). However, it is unclear whether EndoA is involved in yolk endocytosis during oogenesis.…”

Section: Introductionmentioning

confidence: 99%

lncR26319/miR‐2834/EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

Wang

Cheng

et al. 2022

Insect Science

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Oocyte maturation is critical for insect reproduction. Vitellogenesis, the timely production and uptake of vitellogenin (Vg), is crucial for female fecundity. Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis. In the silkworm, Bombyx mori, we discovered that a nucleus-enriched long-noncoding RNA (lncRNA) lncR26319 regulates Endophilin A (EndoA) -a member of the endophilin family of endocytic proteins -through competitive binding to miR-2834. The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm; loss of EndoA or overexpression of miR-2834 significantly reduced egg numbers in virgin moths. In addition, accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females. The expression of Vg, 30-kDa (30K) protein, and egg-specific protein (Esp) decreased after knockdown of EndoA or overexpression of miR-2834, while knockdown of miR-2834 had an opposite effect on the expression of Vg, 30K protein gene, and Esp. These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in the Vg uptake and leads to the normal progression of oogenesis in the silkworm. Thus, miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.

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“…In our study, we found that RNAi-mediated deletion of Endophilin B1 resulted in underdeveloped ovaries, and decreased fecundity, which is consistent with the results that showed that endophilin B could regulate progression of vitellogenesis in Drosophila. 67 However, injection of miR-311-3p agomir had no effect on the expression of Vg in the fat body at the transcriptional level, possibly as a result of the high expression of the miR-311-3p in the ovary and primary involvement in the uptake of yolk by receptor-mediated endocytosis, thus controlling ovarian development and fertility. The abundance of yolk protein in the hemolymph needs to be measured in a further study.…”

Section: Discussionmentioning

confidence: 98%

“…Drosophila Endophilin B acts in Oskar-induced endocytosis that facilitates yolk protein deposition into the oocyte and acceleration of vitellogenesis and thus improves fecundity. 67,68 Indeed, the expression level of Endophilin B was positively correlated with egg production in Drosophila; a high level of Endophilin B resulted in a 30% increase in fecundity by promoting endocytosis in the oocyte. 65 However, miRNAs that target Endophilin B to regulate reproduction have not been identified in Drosophila.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent dual‐luciferase experiments further confirmed that Endophilin B1 was the target gene of miR‐311‐3p in vitro . Drosophila Endophilin B acts in Oskar‐induced endocytosis that facilitates yolk protein deposition into the oocyte and acceleration of vitellogenesis and thus improves fecundity 67,68 . Indeed, the expression level of Endophilin B was positively correlated with egg production in Drosophila ; a high level of Endophilin B resulted in a 30% increase in fecundity by promoting endocytosis in the oocyte 65 .…”

Section: Discussionmentioning

confidence: 99%

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RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

Zhang

et al. 2022

Pest Management Science

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Background The oriental fruit fly, Bactrocera dorsalis, is a highly invasive pest in East Asia and the Pacific. With the development of pesticides resistance, environment‐friendly pesticides are urgently needed. MicroRNAs (miRNAs) are critical regulators of numerous biological processes, including reproduction. Thus, it is significant to identify reproductive‐related miRNAs in this notorious pest to facilitate its control, such as RNAi‐based biopesticides targeting essential miRNAs. Results A high‐throughput sequencing was carried out to identify miRNAs involved in reproduction from the ovary and fat body at four developmental stages [1 day (d), 5, 9, and 13 days post‐eclosion] in female B. dorsalis. Results showed that 98 and 74 miRNAs were differentially expressed in ovary and fat body, respectively, during sexual maturation. Gene ontology analysis showed that target genes involved in oogenesis and lipid particle accounted for 33% and 15% of the total targets, respectively. Among these differentially expressed miRNAs, we found by qPCR that miR‐311‐3p was enriched in the ovary and down‐regulated during sexual maturation. Injection of agomir‐miR‐311‐3p resulted in arrested ovarian development, reduced egg deposition and progeny viability. Endophilin B1 was confirmed to be the target of miR‐311‐3p, via dual‐luciferase assay and expression profiling. Knockdown of Endophilin B1 resulted in reproductive defects similar to those caused by injection of miR‐311‐3p agomir. Thus, miR‐311‐3p might play a critical role in female reproduction by targeting Endophilin B1. Conclusion Our data not only provides knowledge on the abundance of reproductive‐related miRNAs and target genes, but also promotes new control strategies for this pest. © 2022 Society of Chemical Industry.

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Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar

Cited by 6 publications

References 54 publications

LOTUS-domain proteins - developmental effectors from a molecular perspective

LOTUS-domain proteins - developmental effectors from a molecular perspective

lncR26319/miR‐2834/EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

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