Endophyte-mediated resistance against white pine blister rust in Pinus monticola

Ganley, Rebecca J.; Sniezko, Richard A.; Newcombe, George

doi:10.1016/j.foreco.2008.01.052

Cited by 127 publications

(106 citation statements)

References 30 publications

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“…By the production of specific metabolites and biologically active chemicals which strengthen the plant, it reduces the level of herbivore damage, e.g., gall insects. A successful interaction can also be characterized by an increased resistance of the host against pathogens by "controlling" these pathogens (e.g., white pine blister rust in Pinus monticola -Ganley et al 2008). For the microorganisms the advantage may be the persistence in a host over a longer period and the dispersal with the next host generation up to the moment the host's living conditions change (Saikkonen et al 1998).…”

Section: Resultsmentioning

confidence: 99%

Endophytes in changing environments - do we need new concepts in forest management?

Krabel¹,

Morgenstern²,

Herzog³

2013

iForest

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Section: Resultsmentioning

confidence: 99%

Endophytes in changing environments - do we need new concepts in forest management?

Krabel¹,

Morgenstern²,

Herzog³

2013

iForest

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“…Controlled inoculation studies have shown that particular fungal leaf endophyte species can reduce disease in their plant hosts (Arnold et al 2003, Ganley et al 2008, Raghavendra and Newcombe 2013. Variation in host plant genotype is also well known to influence disease: genetic resistance, which can vary among plant genotypes, strongly determines pathogen symptom severity (Jones and Dangl 2006).…”

Section: Introductionmentioning

confidence: 99%

Leaf endophytes and Populus genotype affect severity of damage from the necrotrophic leaf pathogen, Drepanopeziza populi

et al. 2013

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Abstract. Fungal leaf endophytes-nonpathogenic microfungi that live within plant leaves-are ubiquitous in land plants. Leaf endophytes and host plant genotypes may interact to determine plant disease severity. In a greenhouse inoculation experiment, we found that leaf endophyte species and Populus angustifolia genotypes both affected disease outcomes in plants inoculated with the necrotrophic leaf pathogen Drepanopeziza populi. Contrary to many studies showing endophytes conferring defense, all plant genotypes inoculated with the endophyte Penicillium sp. prior to inoculation with the pathogen D. populi were characterized by greater pathogen symptom severity than plants inoculated with the pathogen only. We quantified defense gene expression via qRT-PCR, but found no evidence that increased pathogen damage was related to differential expression of the assayed genes. A second endophyte, Truncatella angustata, which was previously found to reduce symptom severity of the biotrophic pathogen Melampsora in Populus trichocarpa, did not affect symptom severity of the necrotrophic pathogen D. populi or defense gene expression. Overall, our study highlights the variable effects of endophytes on pathogen symptom severity, and illustrates that plant genotypic variation can remain important for disease outcomes even in the presence of endophytes altering disease. Additional work is needed to elucidate the mechanism by which fungal leaf endophytes alter disease in their host plants.

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“…The development of PCR primers specific to the target organism is one of the most important steps in the PCR assay. The ITS regions of rDNA have been widely used to design specific primers for the identification of fungi of interest due to their high copy number and the fact that they contain both conserved and variable regions (Lovic et al, 1995;Ghignone et al, 2003;Stenström & Ihrmark, 2005;Langrell, 2011;Lin et al, 2014). In addition, a large amount of rDNA sequence data from a variety of fungi is available in public databases.…”

Section: Discussionmentioning

confidence: 99%

“…Some endophytic fungi are latent pathogens that cause disease under certain conditions (Begoude et al, 2011;Sakalidis et al, 2011;Stanosz et al, 2001), while others can enhance host performance by conferring resistance to pathogens (Ganley et al, 2008;Romeralo et al, 2015). In addition, some endophytic fungi in the needles of conifers have been reported to be decomposers of needle litter (Müller et al, 2001;Korkama-Rajala et al, 2008;Osono & Hirose, 2011;Yuan & Chen, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a variety of molecular tools have been used to differentiate among fungal species. Among these, species-specific polymerase chain reaction (PCR) has emerged as a powerful tool for the identification and detection of phytopathogenic fungi, such as root rot pathogen Rhizopycnis vagum (Ghignone et al, 2003), collar rot pathogen Sclerotium rolfsii (Pravi et al, 2014), chestnut blight pathogen Cryphonectria parasitica (Popov et al, 2010), and pine needle pathogen Lophodermium spp. (Stenström & Ihrmark, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>

Kihara¹,

Ueno²,

Arase³

2015

OJF

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A specific and sensitive polymerase chain reaction (PCR) assay based on the internal transcribed spacer (ITS) region of rDNA sequences was developed to detect endophytic and phytopathogenic fungi from needles of the Japanese black pine, Pinus thunbergii. Sequences of the ITS regions of Lophodermium conigenum, Lecanosticta acicola, Pestalotiopsis neglecta, Rhizosphaera kalkhoffii, and Septorioides pini-thunbergii were compared, and each specific primer pair for these species was designed. First, the designed primer pairs were tested for their specificity to detect each species. A PCR product was amplified only each combination of species and its specific primer pair, confirming the specificity of the designed primer pairs. These primer pairs were also tested on DNA extracted from the needles of P. thunbergii. The PCR products were amplified not only in needles with lesions but also in healthy needles without symptoms. Furthermore, several endophytic and phytopathogenic fungi could be simultaneously detected from the same region in a needle. The PCR-mediated detection method developed in this study will be a valuable tool for the detection of the endophytic and phytopathogenic fungi, not only as a rapid diagnostic tool for early detection but also for monitoring variations in both the quality and quantity of the endophytic and phytopathogenic fungi in needles in Japanese black pines.

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Endophyte-mediated resistance against white pine blister rust in Pinus monticola

Cited by 127 publications

References 30 publications

Endophytes in changing environments - do we need new concepts in forest management?

Endophytes in changing environments - do we need new concepts in forest management?

Leaf endophytes and Populus genotype affect severity of damage from the necrotrophic leaf pathogen, Drepanopeziza populi

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>

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Endophyte-mediated resistance against white pine blister rust in Pinus monticola

Cited by 127 publications

References 30 publications

Endophytes in changing environments - do we need new concepts in forest management?

Endophytes in changing environments - do we need new concepts in forest management?

Leaf endophytes and Populus genotype affect severity of damage from the necrotrophic leaf pathogen, Drepanopeziza populi

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, &lt;i&gt;Pinus thunbergii&lt;/i&gt;

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PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>