Endophytic bacteria isolated from Solanum nigrum L., alleviate cadmium (Cd) stress response by their antioxidant potentials, including SOD synthesis by sodA gene

Ullah, Ihsan; Johny, Bassam Oudh Al; Al‐Ghamdi, Khalid A.; Al-Zahrani, Hind A. A.; Anwar, Yasir; Firoz, Ahmad; Alkenani, Naser A.; Almatry, Mohammed Ali Ahmed

doi:10.1016/j.ecoenv.2019.02.074

Cited by 90 publications

(22 citation statements)

References 40 publications

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“…Suppressive influence of salinity stress on plant germination has been associated with a reduced level of endogenous hormones. Endophytic originated plant growth regulators have been found to have same effects in plant as of exogenous phytohormones (Ullah et al 2019). In this regard, the SAK1, used in present study, could be a new addition in batch of plant stress hormone-regulating endophytic bacteria (PSHEB).…”

Section: Discussionmentioning

confidence: 89%

Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1

et al. 2019

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Background Salinity has been a major abiotic stressor that reduce the productivity. Previous studies reported that endophytic bacteria produce plant stress response hormones, antioxidants, and enzymes such as ACC deaminase. Augmentation of these metabolites and enzymes by endophytes mitigates the stress effects of salinity and improves plant growth and productivity. Methods Bacterial endophytes were isolated from Artemisia princeps Pamp, and evaluated for indole-3-acetic acid (IAA), abscisic acid (ABA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase production and the ability to solubilize phosphate in the presence of NaCl (100-400 mM). SAK1 was applied to Glycine max cv. Pungsannamul to investigate salinity stress. Results Our results revealed that with an increase in NaCl concentration, the amount of ABA production in SAK1 increased, whereas IAA levels decreased. Bacterial ABA and JA degrade the reactive oxygen species and protect plants against stressors. Gas chromatography-mass spectrometry (GC-MS) analysis detected different gibberellins (GAs) and organic acids in SAK1. Interestingly, SAK1 inoculation significantly increased plant growth attributes under normal and salinity stress conditions, whereas a decrease in endogenous jasmonic acid and ABA content in the plants was recorded under salinity stress. IAA and GAs enhance number of root tips and hence improve nutrients uptake in plants. Polyphenolic oxidase and peroxidase were alleviated by elevated SAK1 in G. max plants under stress. ACC deaminase of SAK1 resulted deamination of ACC, up to 330 nmol α-ketobutyrate mg −1 h −1 which could be a major reason of ethylene reduction promoting plant growth. Conclusion SAK1 relieved salinity stress in plants by producing different phytohormones, antioxidants, and ACC deaminase enzyme. SAK1 could be a new addition in batch of plant stress hormone-regulating endophytic bacteria that mitigates the effects of salt stress and promotes plant growth in G. max.

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Section: Discussionmentioning

confidence: 89%

Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1

et al. 2019

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“…300 grams per pot), whereas one set of plants were treated with cadmium, and the last set of cadmium exposed plants were inoculated with the plant growth promoting (PGPR) endophytic bacteria Serratia sp. IU01 (Ullah et al, 2019). Plants were exposed to cadmium stress @ 1000 mg/100ml & 2000mg/100ml of cadmium nitrate (Cd(NO3)2) and endophytic microbial interaction with Serratia sp.…”

Section: Methodsmentioning

confidence: 99%

“…Many reports have demonstrated that some of the hyper-accumulator plants, including Solanum nigrum L. harbor special endophytes that can tolerate higher concentrations of heavy metals such as cadmium (Chen et al, 2014). A number of cadmium accumulator endophytes have been isolated from Solanum nigrum L. and subsequently applied to alleviate cadmium toxicity in different plant species (Chen et al, 2010;Xiao et al, 2010;Vinichuk et al, 2013;Ullah et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…It is one of the most suitable phytoaccumulator plants that has been used in heavy metal contaminated areas to clean up the environment (Angelova et al, 2017). The reason for using this specific bacterial isolate was that it was shown earlier to enhance plant growth and cadmium tolerance in a closely related species (Ullah et al, 2019). The potential of an endophytic bacterium in terms of plant growth promotion, tolerance, and detoxification of cadmium was assessed by inoculating two genotypes of Brassica napus exposed to different concentrations of cadmium with this isolate.…”

Section: Introductionmentioning

confidence: 99%

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Inoculation of Brassica Napus L. Genotypes With Endophytic Bacteria Promote Growth and Alleviate Cadmium Toxicity

2020

THE JAPS

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Cadmium is a non-essential heavy metal that is highly toxic for most of the plants, even at low concentrations. Cadmium tolerant endophytic bacteria isolated from different sources have been effectively used for mitigating cadmium toxicity and plant growth promotion. In the present study, the potential of an endophytic bacterial strain known as Serratia sp. IU01, previously isolated from Solanum nigrum, was evaluated in terms of alleviating cadmium toxicity and promoting host plant growth. A significant increase in the biomass of two genotypes of Brassica napus L. exposed to different concentrations of cadmium was observed after three weeks inoculation with Serratia sp. IU01 compared to sole cadmium treated plants. High performance liquid chromatography (HPLC) analysis revealed the steady-levels of one of the key antioxidants, i.e., glutathione, were significantly higher in the sole cadmium treated plants compared to control plants. However, inoculation of the cadmium treated plants with the bacterial endophyte resulted in a significant reduction in glutathione contents compared to sole cadmium treated plants, which suggest the antioxidants released by the endophyte in the host environment have assisted the host plant in minimizing the magnitude of the resultant oxidative damage. The precursor of glutathione, i.e., cysteine, showed a similar pattern in the sole cadmium treated and inoculated plants. Our data suggested that inoculation of Brassica napus with the bacterial isolate Serratia sp. IU01, under cadmium stress provide a physiological advantage to the plants in terms of growth promotion and alleviating cadmium toxicity.

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“…These organisms create a positive environment that can help in stress 64 management. Plant growth-promoting bacteria (PGPB) can enhance plant growth and protect plants from diseases and abiotic stresses through a wide variety of mechanisms, including deaminase activity, nodule formation, siderophore production, organic acid production, and other physiological activities that help the plants to tolerate stress (Ullah et al, 2019). Several researchers (Tak et al, 2013;Kumar and Verma, 2018) have reported that the efficacy of plant growth-promoting rhizobacteria (PGPR) reduces under sudden changes of environmental conditions and they fail to respond in the field due to stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Assessment of plant growth-promoting stress-tolerant endophytes screened from Bougainvillea glabra for the growth of Triticum aestivum L. and Zea mays

Karnwal

2020

bta

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Globally, more than 5.2 billion hectares of farming fields are damaged through erosion, salinity, and soil deterioration. Salt stress-tolerant bacteria have plant growth-promoting (PGP) characteristics that can be used to overcome environmental stresses. Isolation and screening of salt-tolerant endophytes from Bougainvillea glabra were achieved through surface sterilization of leaves, followed by their cultivation on 0.5% NaCl-supplemented media. The performance of isolates for indole acetic acid (IAA) production, phosphate solubilization, ACC deaminase activity, ammonia production, siderophore production, and stress tolerance was determined. In the present study, 5 morphologically distinct salt-tolerant endophytic bacteria were cultured. Out of 5 isolates, 60% salt-tolerant endophytes showed phosphate solubilization, 100% IAA production, 40% ACC-deaminase activity, 100% siderophore production, 40% ammonia production and 60% HCN production. Dendrogram generated based on stress tolerance showed three clusters: clusters 1 and 2 containing two isolates and cluster 3 containing 1 isolate. Based on the highest PGP activities, BoGl17 and BoGl21 isolates were chosen and further tested for wheat and corn growth promotion. The isolates increased wheat shoot and root dry weight by 40% and 80%, and by 69% and 126% for BoGl17 and BoGl21, respectively. The bacterial isolates identified as Pseudomonas reidholzensis BoGl17 had 95.59% identity with Pseudomonas reidholzensis strain ID3, while the isolate Bacillus aerius BoGl21 had 90.96% identity with Bacillus aerius strain 24K based on phenotypic features and 16S rRNA gene sequencing results. Both isolates significantly improved plant growth as compared to uninoculated plants.

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Endophytic bacteria isolated from Solanum nigrum L., alleviate cadmium (Cd) stress response by their antioxidant potentials, including SOD synthesis by sodA gene

Cited by 90 publications

References 40 publications

Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1

Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1

Inoculation of Brassica Napus L. Genotypes With Endophytic Bacteria Promote Growth and Alleviate Cadmium Toxicity

Assessment of plant growth-promoting stress-tolerant endophytes screened from Bougainvillea glabra for the growth of Triticum aestivum L. and Zea mays

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