Endophytic fungal extracts: evaluation as photosynthesis and weed growth inhibitors

Moura, Mariana de Souza; Lacerda, Jhuly Wellen Ferreira; Siqueira, Kátia Aparecida de; Bellete, Barbara Sayuri; Sousa, Paulo Teixeira de; Dall´Óglio, Evandro L.; Soares, Marcos Antônio; Vieira, Lucas Campos Curcino; Sampaio, Olívia Moreira

doi:10.1080/03601234.2020.1721981

Cited by 15 publications

(15 citation statements)

References 58 publications

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“…Although, D. helianthi, in particular, which is the causal agent of stem canker of sunflower, has been reported to produce the phytotoxin Phomozin (Mazars et al , 1990), no reports have been found to date on the effect of this toxin on the PSII. Other authors showed the effect of D. phaseolorum methanolic extracts on the PSII activity of Senecio occidentalis and Ipomoea grandifolia (Moura et al ., 2020) with PIabs decrease and inhibition in the QB reduction. As the anamorph Phomopsis was used in this work, the presence of toxins in diseased plants could be a subject for future research.…”

Section: Discussionmentioning

confidence: 99%

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Mantz

Rossi

Viretto

et al. 2021

Preprint

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Pecan plants are attacked by the fungus Phomopsis spp. that causes stem canker, a serious and emerging disease in commercial orchards. Stem canker, which has been reported in several countries, negatively affects tree canopy health, eventually leading to production losses. The purpose of this study was to inquire into the physiology of pecan plants under stem canker attack by Phomopsis spp. To this end, pecan plants were inoculated with an isolate of Phomopsis spp. and several parameters, such as polyamines, proline, sugars, starch, chlorophyll fluorescence and canopy temperature were analysed. Under artificial inoculation, a high disease incidence was observed with symptoms similar to those in plants showing stem canker under field conditions. Furthermore, the infected stem showed dead tissue with brown necrotic discolouration in the xylem tissue. The free polyamines putrescine, spermidine, and spermine were detected and their levels decreased as leaves aged in the infected plants with respect to the controls. Chlorophyll fluorescence parameters, such as Sm, ψEO, and QbRC decreased under plant infection and therefore the K-band increased. Canopy temperature and proline content increased in the infected plants with respect to the controls while sugar content decreased. These data suggest that stem canker caused by Phomopsis spp. induces physiological changes that are similar to those observed in plants under drought stress. To our knowledge, this is the first study that documents the physiological and biochemical effects derived from pecan-Phomopsis interaction.

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Section: Discussionmentioning

confidence: 99%

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Mantz

Rossi

Viretto

et al. 2021

Preprint

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“…The Chl a fluorescence assay was performed according to the protocol previously described by our research group [13,51,52] . Spinach ( Spinacia oleracea ) leaves were cut into 10 mm diameter discs and 10 of these circles were placed with 20 mL of modified Krebs solution into a Petri dish (9 cm diameter).…”

Section: Methodsmentioning

confidence: 99%

“…The Chl a fluorescence assay was performed according to the protocol previously described by our research group. [13,51,52] Spinach (Spinacia oleracea) leaves were cut into 10 mm diameter discs and 10 of these circles were placed with 20 mL of modified Krebs solution into a Petri dish (9 cm diameter). After a 12 h photoperiod of incubation at room temperature, 100 μM of compounds 4a-e were added in dimethyl sulfoxide (DMSO) solutions to each Petri dish and incubated for an additional 6 h. Then, the discs were kept in the dark for 30 min.…”

Section: Chl a Fluorescence Measurementmentioning

confidence: 99%

Binding Properties of Photosynthetic Herbicides: Photosynthetic Activity and Molecular Docking Approach towards 1,4‐Dihydropyridines Derivatives

Soares

Basso

Santos

et al. 2022

Chemistry & Biodiversity

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In the current work, we describe the synthesis of 1,4‐dihydropyridine (1,4‐DHP) derivatives via Hantzsch multicomponent reaction and their evaluation as photosystem II (PSII) inhibitors through chlorophyll a fluorescence bioassay. Among all the compounds tested, 1,1′‐(2,4,6‐trimethyl‐1,4‐dihydropyridine‐3,5‐diyl)bis(ethan‐1‐one) (4b) showed best results, reducing the parameters performance index on absorption basis (PIabs) and electron transport per reaction center by 61 % and 49 %, respectively, as compared to the control. These results indicate the inhibitory activity of PSII over the electron transport chain. Additionally, a molecular docking approach using the protein D1 (PDB code 4V82) was performed in order to assess the structure‐activity relationship among the 1,4‐DHP derivatives over the PSII, which revealed that both, size of the group at position 4 and the carbonyl groups at the dihydropyridine ring are important for the ligand's interaction, particularly for the hydrogen‐bonding interaction with the residues His215, Ser264, and Phe265. Thus, the optimization of these molecular features is the aim of our research group to extend the knowledge of PSII electron chain inhibitors and the establishment of new potent bioactive molecular scaffolds.

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“…However, a chemical investigation into the association of C. lanceolatum plants with their endophytic fungi has not yet been described in the literature. Among the endophytes isolated and identified from C. lanceolatum plants, [25] Diaporthe phaseolorum ( Dp ) and Trichoderma spirale ( Ts ) fungi have been investigated for a variety of biological properties, such as antioxidant, antimicrobial and herbicidal activities [25–28] . In this context, the aim of this work is the 1 H‐NMR metabolomic analysis of qualitative and quantitative changes in the interaction between the C. lanceolatum plant and the endophytic fungi D. phaseolorum and T. spirale over the course of the interaction.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of Combretum lanceolatum Plants Interaction with Diaporthe phaseolorum and Trichoderma spirale Endophytic Fungi through ¹H‐NMR

Lacerda

Siqueira

Vasconcelos

et al. 2021

Chemistry & Biodiversity

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Endophytic fungi are an important class of microorganisms, able to interact with a host plant via a mutualistic mechanism without visible symptoms of the fungal colonization. The synergy between endophytic fungi and their host plant can promote morphological, physiological and biochemical changes through the expression of bioactive metabolites. This work aims to correlate metabolic changes in the Combretum lanceolatum plant metabolome with its endophytic fungi Diaporthe phaseolorum (Dp) and Trichoderma spirale (Ts), and to discover corresponding metabolite‐biomarkers, with the principal focus being on its primary metabolism. The 1H‐NMR metabolomic analysis of qualitative and quantitative changes was performed through multivariate statistical analysis and the identification of primary metabolites was achieved on the Madison Metabolomics Consortium Database. The presence of Dp significantly impacted the plant's metabolic pathways, improving the biosynthesis of primary metabolites such as threonine, malic acid and N‐acetyl‐mannosamine, which are precursors of special metabolites involved in plant self‐defence. This work represents a valuable contribution to advanced studies on the metabolic profiles of the interaction of plants with endophytes.

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Endophytic fungal extracts: evaluation as photosynthesis and weed growth inhibitors

Cited by 15 publications

References 58 publications

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Binding Properties of Photosynthetic Herbicides: Photosynthetic Activity and Molecular Docking Approach towards 1,4‐Dihydropyridines Derivatives

Metabolomic Analysis of Combretum lanceolatum Plants Interaction with Diaporthe phaseolorum and Trichoderma spirale Endophytic Fungi through ¹H‐NMR

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Endophytic fungal extracts: evaluation as photosynthesis and weed growth inhibitors

Cited by 15 publications

References 58 publications

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Stem canker caused byPhomopsis spp. induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves

Binding Properties of Photosynthetic Herbicides: Photosynthetic Activity and Molecular Docking Approach towards 1,4‐Dihydropyridines Derivatives

Metabolomic Analysis of Combretum lanceolatum Plants Interaction with Diaporthe phaseolorum and Trichoderma spirale Endophytic Fungi through 1H‐NMR

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Metabolomic Analysis of Combretum lanceolatum Plants Interaction with Diaporthe phaseolorum and Trichoderma spirale Endophytic Fungi through ¹H‐NMR