Endophytic Fungi From the Brazilian Flora and Their Employment in Biotransformation Reactions

Santos, Valmore Henrique Pereira dos; Silva, Eliane de Oliveira

doi:10.21577/0100-4042.20170380

Cited by 7 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biotransformation processes mediated by endophytic fungi as enzymes or as whole-cell catalysis result into new pharmaceutically active molecules. Literature reports have supported the remarkable and transferable biochemical potential of endophytic fungi into productive sectors (Santos and Silva, 2019;Fryszkowska and Devine, 2020).…”

Section: Synthesis Of Novel Structural Analogs and Active Pharmaceutical Ingredientsmentioning

confidence: 89%

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Choudhary

Gupta

Dhar

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Catalysis is a process carried out in the presence of a heterogenous catalyst for accelerating the rate of a chemical reaction. It plays a pivotal role in transition from take, make, and dispose technology to sustainable technology via chemo- and biocatalytic processes. However, chemocatalyzed reactions are usually associated with copious amounts of perilous/hazardous environmental footprints. Therefore, whole-cell biotransformations or enzyme cocktails serve as cleaner biocatalytic alternatives in replacing the classical chemical procedures. These benchmark bioconversion reactions serve as important key technology in achieving the goals of green chemistry by eliminating waste generation at source. For this, nature has always been a driving force in fuelling natural product discovery and related applications. The fungal endophytic community, in particular, has undergone co-evolution with their host plant and has emerged as a powerful tool of genetic diversity. They can serve as a treasure trove of biocatalysts, catalyzing organic transformations of a wide range of substances into enantiopure compounds with biotechnological relevance. Additionally, the biocatalytic potential of endophytic fungi as whole-intact organisms/isolated enzyme systems has been greatly expanded beyond the existing boundaries with the advancement in high-throughput screening, molecular biology techniques, metabolic engineering, and protein engineering. Therefore, the present review illustrates the promising applications of endophytic fungi as biocatalysts for the synthesis of new structural analogs and pharmaceutical intermediates and refinement of existing proteins for novel chemistries.

show abstract

Section: Synthesis Of Novel Structural Analogs and Active Pharmaceutical Ingredientsmentioning

confidence: 89%

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Choudhary

Gupta

Dhar

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…They can serve as a treasure trove of biocatalysts, catalyzing organic transformations of a wide range of substances into enantiopure compounds with biotechnological relevance. Endophytic fungi not only exhibit their degradation ability within the host plant but also display high degradation activity outside the plant (Santos & Silva 2019;.…”

Section: Introductionmentioning

confidence: 99%

Biotransformation potential of the R-(+)-limonene by endophytic fungi strain associated with Amazonian fruit Malpighia emarginata DC

Ferreira dos Santos,

Daniel Gonçalves Pena,

Muniz Nunes

et al. 2023

CLIUM

View full text Add to dashboard Cite

This study aims to offer an account of the biotransformation of limonene through the endophytic fungi strains associated with the Amazonian fruit Malpighia emarginata. The fungal biotransformation was executed in flasks containing the substrate R-(+)-limonene in a mineral medium, which was further incubated at 28 °C on a rotary shaker (120 rpm) for 120 h. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed using gas chromatography–mass spectrometry with the National Institute of Standards and Technology database. In this study with five endophytic fungi strains, only Penicillium sp. AF- LAB4 was considered a potential biocatalyst found in the screening due to its capacity to use the R-(+)-limonene as the single carbon and energy source in a mineral medium since they eventually accumulated interesting compound as limonene-1,2-diol (128.10 μL/L) after 48 to 120 h of reaction. This achievement is important and supports the development of the production of natural aromas and demonstrates the potential of using this endophytic fungus strain from the fruit M. emarginata, as a new biocatalyst.

show abstract

Endophytic fungus from Handroanthus impetiginosus immobilized on electrospun nanofibrous membrane for bioremoval of bisphenol A

Conceição¹,

Alvarega²,

Mercante³

et al. 2023

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The current industrial and human activities scenario has accelerated the widespread use of endocrinedisrupting compounds (EDCs), which can be found in everyday products, including plastic containers, bottles, toys, cosmetics, etc., but can pose a severe risk to human health and the environment. In this regard, fungal bioremediation appears as a green and cost-effective approach to removing pollutants from water resources. Besides, immobilizing fungal cells onto nano brous membranes appears as an innovative strategy to improve remediation performance by allowing the adsorption and degradation to occur simultaneously. Herein, we developed a novel nanostructured bioremediation platform based on polyacrylonitrile nano brous membrane (PAN NFM) as supporting material for immobilizing an endophytic fungus to remove bisphenol A (BPA), a typical EDC. The endophytic strain was isolated from Handroanthus impetiginosus leaves and identi ed as Phanerochaete sp. H2 by molecular methods. The successful assembly of fungus onto the PAN NFM surface was con rmed by scanning electron microscopy (SEM). Compared with free fungus cells, the PAN@H2 NFM displayed a high BPA removal e ciency (above 85%) at an initial concentration of 5 ppm, suggesting synergistic removal by simultaneous adsorption and biotransformation. Moreover, the biotransformation pathway was investigated, and the chemical structures of fungal metabolites of BPA were identi ed by ultra-high performance liquid chromatography -high-resolution mass (UHPLC-HRMS) analysis. In general, our results suggest that by combining the advantages of enzymatic activity and nano brous structure, the novel platform has the potential to be applied in the bioremediation of varied EDCs or even other pollutants found in water resources.

show abstract

Endophytic Fungi From the Brazilian Flora and Their Employment in Biotransformation Reactions

Cited by 7 publications

References 30 publications

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Biotransformation potential of the R-(+)-limonene by endophytic fungi strain associated with Amazonian fruit Malpighia emarginata DC

Endophytic fungus from Handroanthus impetiginosus immobilized on electrospun nanofibrous membrane for bioremoval of bisphenol A

Contact Info

Product

Resources

About