Endophytic fungi from Vitis labrusca L. ‘Niagara Rosada’) and its potential for the biological control of Fusarium oxysporum

Brum, Maria Cristina Pires de; Araújo, Welington Luiz; Maki, Cristina Sayuri; Azevedo, João Lúcio

doi:10.4238/2012.december.6.2

Cited by 58 publications

(38 citation statements)

References 25 publications

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“…The latter species were also reported in studies by Mostert et al (2000) and Gonzáles and Tello (2011). In the isolation of endophytes of V. labrusca, Brum et al (2012) observed the presence of the general fungi Diaporthe and Fusarium.…”

Section: Discussionsupporting

confidence: 67%

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Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

et al. 2015

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Endophytic fungi colonize the interior of plant tissues and organs, establishing an intimate mutualistic association with no visible symptoms. The fungi may help protect the plant against herbivores and pathogens, making them potentially useful endophytes in the biological control of diseases and agricultural pests. The biotechnological interest in these organisms has stimulated research related to the bioprospecting of endophytic fungi. Grapevine is among the oldest of plants cultivated by man, with the grape being one of the most highly consumed fruits in the world. Diseases cause significant damage to grape cultures, making their integrated control important to reduce the use of pesticides and, consequently, environmental and human contamination. The rustic species Vitis labrusca L. (Vitaceae), used in the preparation of juices and wines, is highly resistant to fungal diseases. We isolated leaf endophytic fungi of the Bordô and Concord cultivars (V. labrusca L.), which were ordered into 68 and 62 morpho-groups of the Bordô and Concord cultivars, respectively. We used scanning electron microscopy to confirm the presence of endophytes in the leaves. Endophytic diversity was analyzed based on sequencing the ITS1-5.8S-ITS2 region of rDNA, allowing the identification of fungi belonging to genera including Cochliobolus, Bipolaris, Fusarium, Alternaria, Diaporthe, Phoma and Phomopsis. Phylogenetic analysis confirmed the identity of the endophytes. The biotechnological potential of endophytes was tested in vitro for the control of pathogenic fungi of grapevines including Alternaria sp., Sphaceloma sp. and Glomerella sp. Inhibition percentages above 50 % as demonstrated by some isolates demonstrate their potential for biological control.

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Section: Discussionsupporting

confidence: 67%

“…However, most studies have concentrated on fungi associated with V. vinifera (Mostert et al 2000;Burruano et al 2008;Casieri et al 2009;Gonzáles and Tello 2011;Pancher et al 2012). Studies related to the bioprospecting of endophytic fungi in V. labrusca species are less common (Lima 2010;Brum et al 2012).…”

Section: Introductionmentioning

confidence: 96%

Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

et al. 2015

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“…In a study of Vitis labrusca in the São Paulo region, Brazil, 48.14% of the endophyte fungi isolates belonged to phylum Ascomycota (Brum et al, 2012). In the present study, all of the isolates belonged to this phylum, suggesting that they may be involved in the protection of the host plant against fungal diseases.…”

Section: Discussionmentioning

confidence: 75%

“…Although it is known for its pathogenicity, it also includes non-pathogenic species that are effective for biological control of phytopathogenic fungi. However, species such as Fusarium oxysporum can produce toxins and cause plant diseases, thereby invalidating the use of Fusarium for biocontrol because of the risks of food contamination (Brum et al, 2012). In the present study, the phytopathogenic and biocontrol capacities of this genus were not evaluated.…”

Section: Discussionmentioning

confidence: 99%

Isolation and genetic characterization of endophytic and rhizospheric microorganisms from Butia purpurascens Glassman

Silva¹,

Senabio²,

Pinheiro³

et al. 2015

Afr. J. Microbiol. Res.

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Interactions between plants and microorganism are complex and can affect the establishment of plant communities and change ecosystem properties. These interactions are of interest for researchers and have many ecological and biotechnological applications. The present study is the first report on the microbial diversity associated with Butia purpurascens Glassman, and the goal was to isolate and identify the genetic diversity of endophytic and rhizospheric growable microorganisms from B. purpurascens using molecular biology techniques. Endophytic and rhizospheric microorganisms were isolated from the roots and rhizospheric soil of the purple yatay palm. DNA was extracted, and the 16S region for bacteria and internal transcribed spacer (ITS) region for fungi were amplified and sequenced. The resulting sequences were compared with known sequences from GenBank by similarity search using BLASTn. The rhizosphere and roots of B. purpurascens harbor a diverse set of microorganism groups. Fourteen (14) genera of endophytic and 12 genera of rhizospheric bacteria were identified that belonged to three phyla (Proteobacteria, Firmicutes and Actinobacteria), and 11 endophytic and six rhizospheric genera of fungi were identified that belonged to the phylum Ascomycota. The most frequent isolated genera were Enterobacter and Pseudomonas for endophytic bacteria, Gibberella and Codinaeopsis for endophytic fungi, Bacillus and Enterobacter for rhizospheric bacteria, and Ceratocystis for rhizospheric fungi. Differences were observed between the endophytic and rhizospheric microbial communities of B. purpurascens, with some microorganisms only detected in one environment. Further studies with a higher number of individuals are required to confirm these results.

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“…For instance, Acremonium sp., an endophyte of Zingiber officinale, was studied for its antagonistic activity against Pythium myritylum causing soft rot of ginger [14]. Similarly, antifungal activity of endophytes of grapevine has been checked against the pathogen of grape vine [59]. Favaro et al have also investigated the antagonistic activity of fungal endophytes isolated from sugarcane against its fungal pathogens [60].…”

Section: Evaluation Of Endophytic Fungal Isolates and Crude Fungal Exmentioning

confidence: 98%

Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

Gupta

Kaul

Singh

et al. 2016

Appl Biochem Biotechnol

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Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped into seven genera based on morphological and molecular data. The fungal endophytes of C. longa were evaluated for antifungal activity against Colletotrichum gloeosporioides, the causal organism of leaf spot of turmeric. The disease is a major cause for economic loss in turmeric cultivation. Endophytic Phoma herbarum showed significant activity against C. gloeosporioides and was therefore selected for further studies. A compound gentisyl alcohol was isolated from P. herbarum which showed effective antagonism against C. gloeosporioides. The organism could therefore be used as a biocontrol agent against C. gloeosporioides.

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Endophytic fungi from Vitis labrusca L. ‘Niagara Rosada’) and its potential for the biological control of Fusarium oxysporum

Cited by 58 publications

References 25 publications

Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

Isolation and genetic characterization of endophytic and rhizospheric microorganisms from Butia purpurascens Glassman

Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

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