Endoplasmic reticulum Ca2+-homeostasis is altered in small and non-small cell lung cancer cell lines

Bergner, Albrecht; Kellner, Julia; Tufman, Amanda; Huber, Rudolf M.

doi:10.1186/1756-9966-28-25

Cited by 46 publications

(44 citation statements)

References 21 publications

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“…A decrease in ER Ca 2+ content has been detected in a subset of lung cancer cell lines when compared to normal human bronchial epithelial cells (Bergner et al, 2009). Consistent with the reduction in ER Ca 2+ in cancer, a reduction in expression of SERCA pump has been observed in cancer-derived cell lines and in tumours.…”

Section: Discussionsupporting

confidence: 54%

Oncogenic K-Ras suppresses IP3-dependent Ca2+ release through remodeling of IP3Rs isoform composition and ER luminal Ca2+ levels in colorectal cancer cell lines

Pierro

Cook

Foets

et al. 2014

Journal of Cell Science

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Section: Discussionsupporting

confidence: 54%

Oncogenic K-Ras suppresses IP3-dependent Ca2+ release through remodeling of IP3Rs isoform composition and ER luminal Ca2+ levels in colorectal cancer cell lines

Pierro

Cook

Foets

et al. 2014

Journal of Cell Science

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“…29 Reduction in expression of calreticulin in lung cancer cell lines was associated with an increased rate of proliferation. 30,31 In a recent preliminary study of 33 breast cancer patients, Erić et al 32 observed a potential correlation of calreticulin overexpression with axillary lymph node metastasis in breast cancer patients.…”

Section: Discussionmentioning

confidence: 99%

“…40 Matricellular protein thrombospondin 1, when bound to calreticulin, induces resistance to anoikis in fibroblasts. 41 Cancer cells are known to acquire enhanced survival mechanisms, which include regulating calcium homeostasis in the endoplasmic reticulum 30 to counter hypoxia and endoplasmic reticulum stress. 42 Calreticulin, being the main alleviator of endoplasmic stress, 43 could therefore have a key role in the survival of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological significance of calreticulin in breast invasive ductal carcinoma

et al. 2010

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Calreticulin is a chaperone protein located in the lumen of the endoplasmic reticulum. The association of calreticulin with pathological conditions such as autoimmune disorders and certain types of cancer have been reported. However, little is known about its role in the pathogenesis of breast cancer. The aim of this study was to determine the expression of calreticulin in vitro and correlate its expression levels in breast cancer tissue samples with clinicopathological parameters. Calreticulin expression was evaluated in MCF-7 and MDA-MB-231 breast cancer cells by real-time RT-PCR, Western blot, immunohistochemistry, and immunofluorescence staining. Patient tissue microarrays were constructed from 228 breast cancer specimens for immunohistochemical analysis. The in vitro study showed a higher calreticulin expression in more aggressive MDA-MB-231 cells as compared with MCF-7 cells at both mRNA and protein levels. In all, 227 out of 228 breast cancer samples exhibited calreticulin staining in at least 5% of the cancer cells. Calreticulin immunostaining was observed to be localized to the cytoplasm of the cancer cells. Regression analysis of calreticulin immunostaining in the tissue microarrays revealed that its expression was positively correlated to logarithm of (log) tumor size (P ¼ 0.046) and development of distant metastasis (P ¼ 0.017). Multivariate analysis confirmed calreticulin expression as an independent predictor of log tumor size and occurrence of distant metastasis. The data suggest that calreticulin expression is associated with more advanced tumors and is a potential prognostic biomarker.

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“…Further, CRT was also found to be differentially expressed in colorectal cancer [14] and breast cancer [15,16]. As to lung cancer, it has been reported that decreased CRT expression in lung cancer cell lines was associated with an increased rate of proliferation of lung cancer cells [17], which indicated the potential application of CRT in diagnosis and therapy of lung cancer. Recently, Soroush Merchant's group found that the surface expression of CRT was highly increased after 1 h Photofrin TM -photodynamic therapy (PDT) treatment in Lewis lung carcinoma (LLC) cells [18].…”

Section: Introductionmentioning

confidence: 96%

“…There have been reports on the association between CRT expression level and various diseases [9][10][11][12][13][14][15][16][17]. It has been detected that there were higher levels of sCRT in the sera of patients with autoimmune disease such as rheumatoid arthritis and systemic lupus erythematosus [9].…”

Section: Introductionmentioning

confidence: 98%

Calreticulin as a potential diagnostic biomarker for lung cancer

Liu

Gong

Chen

et al. 2011

Cancer Immunol Immunother

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Calreticulin (CRT) is an endoplasmic reticulum luminal Ca(2+)-binding chaperone protein. By immunizing mice with recombinant fragment (rCRT/39-272), six clones of monoclonal antibodies (mAbs) were generated and characterized. Based on these mAbs, a microplate chemiluminescent enzyme immunoassay (CLEIA) system with a measured limit of detection of 0.09 ng/ml was developed. Using this CLEIA system, it was found that soluble CRT (sCRT) level in serum samples from 58 lung cancer patients was significantly higher than that from 40 healthy individuals (only 9 were detectable, P < 0.0001). Among them, serum sCRT in the small cell lung cancer was lower than that in adenocarcinoma (P = 0.0085), while both were lower than that in the squamous cell carcinoma (P = 0.013, P = 0.0012, respectively). Moreover, it was found that sCRT in sera from the patients after chemotherapy was higher than that from the patients without chemotherapy (P = 0.042). Further study by immunohistochemistry showed that CRT was also highly expressed in the cytoplasm and on the membrane of the lung cancer cells, while there was a trace amount of CRT expression in normal lung cells. Correspondingly, the expression level of CRT on lung cancer cell membrane was associated with the tumor pathological grade. This study demonstrates that sCRT concentration in sera of lung cancer patients is higher than that in sera of healthy individuals, and CRT expression level on lung cancer cell membrane is associated with tumor pathological classification and grade. These findings suggest that CRT may be used as a biomarker in lung cancer prediction and diagnosis.

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Endoplasmic reticulum Ca2+-homeostasis is altered in small and non-small cell lung cancer cell lines

Cited by 46 publications

References 21 publications

Oncogenic K-Ras suppresses IP3-dependent Ca2+ release through remodeling of IP3Rs isoform composition and ER luminal Ca2+ levels in colorectal cancer cell lines

Oncogenic K-Ras suppresses IP3-dependent Ca2+ release through remodeling of IP3Rs isoform composition and ER luminal Ca2+ levels in colorectal cancer cell lines

Clinicopathological significance of calreticulin in breast invasive ductal carcinoma

Calreticulin as a potential diagnostic biomarker for lung cancer

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