Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Zachariadis, Michael; Quader, H.; Galatis, B.; Apostolakos, P.

doi:10.1007/s004250100563

Cited by 39 publications

(37 citation statements)

References 20 publications

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“…Also, we observe nucleation to occur during anaphase when the nuclear envelope is not completely reformed. For these reasons, it does not seem that the minus end loci are exclusively associated with the nuclear envelope but may be associated, for example, with other components of the endomembrane system such as the endoplasmic reticulum that is known to aggregate at spindle poles upon breakdown of the nuclear envelope (Hepler, 1980;Zachariadis et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

et al. 2005

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In a previous study on Arabidopsis thaliana suspension cells transiently infected with the microtubule end binding protein AtEB1a-green fluorescent protein (GFP), we reported that interphase microtubules grow from multiple sites dispersed over the cortex, with plus ends forming the characteristic comet-like pattern. In this study, AtEB1a-GFP was used to study the transitions of microtubule arrays throughout the division cycle of cells lacking a defined centrosome. During division, the dispersed origin of microtubules was replaced by a more focused pattern with the plus end comets growing away from sites associated with the nuclear periphery. The mitotic spindle then evolved in two quite distinct ways depending on the presence or absence of the preprophase band (PPB): the cells displaying outside-in as well as inside-out mitotic pathways. In those cells possessing a PPB, the fusion protein labeled material at the nuclear periphery that segregated into two polar caps, perpendicular to the PPB, before nuclear envelope breakdown (NEBD). These polar caps then marked the spindle poles upon NEBD. However, in the population of cells without PPBs, there was no prepolarization of material at the nuclear envelope before NEBD, and the bipolar spindle only emerged clearly after NEBD. Such cells had variable spindle orientations and enhanced phragmoplast mobility, suggesting that the PPB is involved in a polarization event that promotes early spindle pole morphogenesis and subsequent positional stability during division. Astral-like microtubules are not usually prominent in plant cells, but they are clearly seen in these Arabidopsis cells, and we hypothesize that they may be involved in orienting the division plane, particularly where the plane is not determined before division.

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Section: Discussionmentioning

confidence: 99%

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

et al. 2005

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“…It is well accepted that the microfilament cytoskeleton is strongly linked to these microtubule structures during cell division, although subtle differences in microfilament distribution have been identified (Panteris, 2008). While some studies have shown that microfilaments codistribute with microtubules in the preprophase band (Kakimoto and Shibaoka, 1987;Palevitz, 1987;Traas et al, 1987;Zachariadis et al, 2001Zachariadis et al, , 2003, others have shown that cortical actin filaments do not always follow the pattern of the preprophase microtubule band Wick, 1990, 1991;Panteris et al, 1992Panteris et al, , 2007Collings and Wasteneys, 2005). In the mitotic spindle, microfilaments are usually present (Schmit and Lambert, 1987;Seagull et al, 1987;Traas et al, 1987;Lloyd and Traas, 1988;Panteris et al, 1992;Cleary, 2001;Collings et al, 2001), but their absence from the spindle has also been reported (Liu and Palevitz, 1992;Baluska and Hasenstein, 1997;Vitha et al, 2000;Voigt et al, 2005).…”

Section: Afh14 Plays An Important Role In Cell Divisionmentioning

confidence: 99%

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

et al. 2010

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Formins have long been known to regulate microfilaments but have also recently been shown to associate with microtubules. In this study, Arabidopsis thaliana FORMIN14 (AFH14), a type II formin, was found to regulate both microtubule and microfilament arrays. AFH14 expressed in BY-2 cells was shown to decorate preprophase bands, spindles, and phragmoplasts and to induce coalignment of microtubules with microfilaments. These effects perturbed the process of cell division. Localization of AFH14 to microtubule-based structures was confirmed in Arabidopsis suspension cells. Knockdown of AFH14 in mitotic cells altered interactions between microtubules and microfilaments, resulting in the formation of an abnormal mitotic apparatus. In Arabidopsis afh14 T-DNA insertion mutants, microtubule arrays displayed abnormalities during the meiosis-associated process of microspore formation, which corresponded to altered phenotypes during tetrad formation. In vitro biochemical experiments showed that AFH14 bound directly to either microtubules or microfilaments and that the FH2 domain was essential for cytoskeleton binding and bundling. However, in the presence of both microtubules and microfilaments, AFH14 promoted interactions between microtubules and microfilaments. These results demonstrate that AFH14 is a unique plant formin that functions as a linking protein between microtubules and microfilaments and thus plays important roles in the process of plant cell division.

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“…In higher plant cells it is generally accepted that ER organization depends on an intact actomyosin system (Quader et al, 1987;Higashi-Fujime, 1988;Kachar & Reese, 1988;Samaj et al, 2004; for a review see Fehrenbacher et al, 2002). However, recent studies have shown that in some higher plant cells, the ER is also organised by microtubules (Mts; Zachariadis et al, 2001;, as in animal cells (Lee & Chen, 1988;Terasaki, 2000).…”

Section: Introductionmentioning

confidence: 99%

Radial endoplasmic reticulum arrays co-localize with radial F-actin in polarizing cells of brown algae

Varvarigos¹,

Galatis²,

Katsaros³

2007

European Journal of Phycology

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The organisation of the endoplasmic reticulum (ER) was examined in branching thallus cells and germinating cells derived from regenerated protoplasts (protoplast derived cells; PDCs) of Macrocystis pyrifera gametophytes by both immunofluorescent labelling of the HDEL sequence and vital staining with DiOC 6 (3), and in branching thallus cells of Splachnidium rugosum and Choristocarpus tenellus with DiOC 6 (3) staining. In M. pyrifera and S. rugosum cells, distinctive cortical ER configurations with a clear radial arrangement were found at sites where a protrusion will emerge, while branching C. tenellus cells had a cortical ER patch from which a few linear ER elements expanded towards the rest of the cell. Examination of the organization of actin filaments (AFs) revealed similar radial AF structures at the site of the future protrusion, while the microtubule (Mt) organisation did not show any similar characteristic structure at the protrusion site. Latrunculin B inhibited germination in M. pyrifera PDCs, and the treated cells lacked radial ER or AF configurations. Treatment with taxol did not affect the germination process or the radial configurations. The structural similarity and the temporal and spatial coincidence of the ER and AF configurations combined with the inhibitor experiments lead to the hypothesis that ER, in conjunction with AFs, is implicated in the fixation of the polar axis in brown algal cells. The existence of characteristic ER and AF configurations in polarising cells of the three species suggests that they are a common characteristic of the brown algae.

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Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Cited by 39 publications

References 20 publications

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

Radial endoplasmic reticulum arrays co-localize with radial F-actin in polarizing cells of brown algae

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