Endoreduplication and gene expression in somaclonal variants of clonally propagated Phalaenopsis ‘Wedding Promenade’

Lee, Hyun-Jeong; Yoon, Yeo-Joong; Paek, Kee-Yoeup; Park, So‐Young

doi:10.1007/s13580-017-0169-2

Cited by 5 publications

(9 citation statements)

References 31 publications

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“…The increase in the starch accumulation recorded in the present study can be attributed to the increase in the expression of AGPase, SSs, GBSS and SBEs genes ( Figure 7 ). The up-regulation of starch synthesis-related genes was documented during tuber formation of potato [ 29 , 46 ]. Based on microarray analysis, Kloosterman et al [ 46 ] recorded up-regulated profile for the genes addressed in the present study during the early stages of tuberization, that was maintained until the final tuber size was reached.…”

Section: Discussionmentioning

confidence: 99%

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

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El-Denary

et al. 2023

Plants

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Starch content is one of the major quality criteria targeted by potato breeding programs. Traditional potato breeding is a laborious duty due to the tetraploid nature and immense heterozygosity of potato genomes. In addition, screening for functional genetic variations in wild relatives is slow and strenuous. Moreover, genetic diversity, which is the raw material for breeding programs, is limited due to vegetative propagation used in the potato industry. Somaclonal variation provides a time-efficient tool to breeders for obtaining genetic variability, which is essential for breeding programs, at a reasonable cost and independent of sophisticated technology. The present investigation aimed to create potato somaclones with an improved potential for starch accumulation. Based on the weight and starch content of tubers, the somaclonal variant Ros 119, among 105 callus-sourced clones, recorded a higher tuberization potential than the parent cv Lady Rosetta in a field experiment. Although this somaclone was similar to the parent in the number of tubers produced, it exhibited tubers with 42 and 61% higher fresh and dry weights, respectively. Additionally, this clone recorded 10 and 75% increases in starch content based on the dry weight and average content per plant, respectively. The enhanced starch accumulation was associated with the upregulation of six starch-synthesis-related genes, namely, the AGPase, GBSS I, SBE I, SBE II, SS II and SS III genes. AGPase affords the glycosyl moieties required for the synthesis of amylose and amylopectin. GBSS is required for amylose elongation, while SBE I, SBE II, SS II and SS III are responsible for amylopectin.

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Section: Discussionmentioning

confidence: 99%

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

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El-Denary

et al. 2023

Plants

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“…Phenotype variants among plantlets may also result from changes in the chromosome numbers, as it has been revealed in Coffea arabica and P. 'Wedding Promenade' (WP) (Lee et al 2017). In C. arabica, phenotypically normal plants have normal chromosome numbers, while the abnormal ones have abnormal chromosomal numbers (Landey et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Reported phenotypic variants among Phalaenopsis plantlets include changes in leaf and shoot characters, ploidy levels and peloric or semi-peloric characters (Chen et al 2008). Such somaclonal variations occurred in P. gigantea (Samarfard et al 2014), P.' Wedding Promenade' (Lee et al 2017), and P. amabilis (Raynalta et al 2018). Meanwhile, somaclonal variation has contributed to the generation of new variants in several ornamental species, such as Saintpaulia (Matsuda et al 2014); Chrysanthemum (Miler and Zalewska 2014); gerbera (Bhatia et al 2009; and Hedicyum coronarium (Parida et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Changes in morphology and ploidy levels may indicate either somaclonal or induced mutations (Chen et al 2008;Lee et al 2017). However, such morphological variations may also be due to epigenetics or environmental factors (Landey et al 2015;Anil et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…However, such morphological variations may also be due to epigenetics or environmental factors (Landey et al 2015;Anil et al 2018). Moreover, somaclonal variation was detected using various techniques, including morphological, cytological, and molecular analyses (Bairu et al 2011;Samarfard et al 2014;Landey et al 2015;Ghimire et al 2016;Sebastiani and Ficcadenti 2016;Lee et al 2017). Genetically controlled somaclonal or induced mutations may be evaluated using various molecular markers, such as RAPD, ISSR, AFLP and SNAP markers or by sequencing of certain target genes (Chen et al 2004(Chen et al , 2008Samarfard et al 2014;Raynalta et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

et al. 2021

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Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.

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The Role of Somaclonal Variation in Plant Genetic Improvement: A Systematic Review

et al. 2023

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The instability of in vitro cultures may cause genetic and epigenetic changes in crops called somaclonal variations. Sometimes, these changes produce beneficial effects; for example, they can be used in breeding programs to generate new cultivars with desirable characteristics. In this article, we present a systematic review designed to answer the following question: How does somaclonal variation contribute to plant genetic improvement? Five electronic databases were searched for articles based on pre-established inclusion and exclusion criteria and with a standardized search string. The somaclonal variation technique has been most frequently applied to ornamental plants, with 49 species cited in 48 articles, and to the main agricultural crops, including sugarcane, rice, banana, potato and wheat, in different countries worldwide. In 69 studies, a technique was applied to evaluate the genetic diversity generated between clones, and, in 63 studies, agronomic performance characteristics were evaluated. Other studies are related to resistance to pathogens, ornamental characteristics and resistance to abiotic stresses. The application of the plant growth regulators (PGRs) benzylaminopurine (BAP) and dichlorophenoxyacetic acid (2,4-D) was the most common method for generating somaclones, and randomly amplified polymorphic DNA (RAPD) molecular markers were the most commonly used markers for identification and characterization. Somaclonal variation has been used in genetic improvement programs for the most economically important crops in the world, generating genetic diversity and supporting the launch of new genotypes resistant to diseases, pests and abiotic stresses. However, much remains to be explored, such as the genetic and epigenetic mechanisms from which somaclonal variation is derived.

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Endoreduplication and gene expression in somaclonal variants of clonally propagated Phalaenopsis ‘Wedding Promenade’

Cited by 5 publications

References 31 publications

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

The Role of Somaclonal Variation in Plant Genetic Improvement: A Systematic Review

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