Endothelial Cell Seeding in the Management of Vascular Thrombosis

Herring, Malcolm B.; Compton, Rebecca S.; LeGrand, Daniel R.; Gardner, Austin

doi:10.1055/s-2007-1002706

Cited by 17 publications

(5 citation statements)

References 28 publications

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“…1,2 We can hypothesize that the latter is caused by both expression of leukocyte adhesion molecules and production of cytokines by ECs activated by the underlying polymer surface.…”

Section: Introductionmentioning

confidence: 99%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

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Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.

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“…1,2 We can hypothesize that the latter is caused by both expression of leukocyte adhesion molecules and production of cytokines by ECs activated by the underlying polymer surface.…”

Section: Introductionmentioning

confidence: 99%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

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show abstract

“…The efficiency of endothelial cell seeding in vascular prostheses would significantly improve with the reduction of endothelial cell detachment after the restoration of circulation. Because in vivo experiments indicate that leukocyte infiltration is involved in endothelial cell loss, 4,5 we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cytokines because of an interaction with the underlying polymer surface. This results in adhesion, extravasation, and activation of leukocytes similar to inflammatory processes.…”

Section: Discussionmentioning

confidence: 99%

“…3 Histological studies in dogs showed severe endothelial cell detachment associated with massive leukocyte infiltration within 6 h after implantation of completely endothelialized vascular prostheses. 4 The observation that the implantation of such grafts in neutropenic dogs resulted in 80% endothelial cell retention after 6 h compared with 30% in untreated controls emphasizes the role of leukocytes. 5 Therefore, we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cyto-kines because of an interaction with the underlying polymer surface.…”

Section: Introductionmentioning

confidence: 99%

Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

Imbert¹,

Poot²,

Figdor³

et al. 2001

J. Biomed. Mater. Res.

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Although endothelial cell seeding in small‐diameter vascular prostheses significantly improves graft survival, the detachment of adherent endothelial cells after the restoration of circulation remains one of the major obstacles. Because in vivo experiments indicate that leukocyte infiltration is involved in endothelial cell loss, we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cytokines because of an interaction with the underlying polymer surface. The aim of this study was to investigate the expression of the leukocyte adhesion molecules ICAM‐1, VCAM‐1, PECAM‐1, and E‐selectin by cultured human umbilical vein endothelial cells (HUVECs) and human adipose microvascular endothelial cells (HAMVECs). The cells were seeded on tissue culture poly(styrene) and the vascular graft materials Dacron and Teflon. The results of this study indicate that the expression of leukocyte adhesion molecules by cultured endothelial cells is mainly affected by the endothelial cell origin, that is, umbilical vein or adipose tissue. Expressions of both ICAM‐1 and E‐selectin by HUVECs and HAMVECs are characterized by the presence of two cell populations with distinct levels of expression. With respect to endothelial cell seeding in vascular prostheses, the increased expression of E‐selectin by microvascular endothelial cells deserves further attention. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 56: 376–381, 2001

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“…The efficiency of endothelial cell seeding in vascular prostheses would significantly improve with the reduction of endothelial cell detachment after the restoration of circulation. Because in vivo experiments indicate that leukocyte infiltration is involved in endothelial cell loss,4, 5 we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cytokines because of an interaction with the underlying polymer surface. This results in adhesion, extravasation, and activation of leukocytes similar to inflammatory processes.…”

Section: Discussionmentioning

confidence: 99%

“…Although the seeding of these prostheses with autologous endothelial cells before implantation would significantly improve graft survival,1, 2 major obstacles include the source of autologous endothelial cells, the efficiency of cell seeding, and the detachment of adherent endothelial cells from the graft surface after the restoration of circulation 3. Histological studies in dogs showed severe endothelial cell detachment associated with massive leukocyte infiltration within 6 h after implantation of completely endothelialized vascular prostheses 4. The observation that the implantation of such grafts in neutropenic dogs resulted in 80% endothelial cell retention after 6 h compared with 30% in untreated controls emphasizes the role of leukocytes 5.…”

Section: Introductionmentioning

confidence: 99%

Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

Imbert

Poot

Figdor

et al. 2001

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Although endothelial cell seeding in small-diameter vascular prostheses significantly improves graft survival, the detachment of adherent endothelial cells after the restoration of circulation remains one of the major obstacles. Because in vivo experiments indicate that leukocyte infiltration is involved in endothelial cell loss, we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cytokines because of an interaction with the underlying polymer surface. The aim of this study was to investigate the expression of the leukocyte adhesion molecules ICAM-1, VCAM-1, PECAM-1, and E-selectin by cultured human umbilical vein endothelial cells (HUVECs) and human adipose microvascular endothelial cells (HAMVECs). The cells were seeded on tissue culture poly(styrene) and the vascular graft materials Dacron and Teflon. The results of this study indicate that the expression of leukocyte adhesion molecules by cultured endothelial cells is mainly affected by the endothelial cell origin, that is, umbilical vein or adipose tissue. Expressions of both ICAM-1 and E-selectin by HUVECs and HAMVECs are characterized by the presence of two cell populations with distinct levels of expression. With respect to endothelial cell seeding in vascular prostheses, the increased expression of E-selectin by microvascular endothelial cells deserves further attention.

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Endothelial Cell Seeding in the Management of Vascular Thrombosis

Cited by 17 publications

References 28 publications

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

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