Endothelial Differentiation Gene-1, a New Downstream Gene Is Involved in RTEF-1 Induced Angiogenesis in Endothelial Cells

Abstract

Related Transcriptional Enhancer Factor-1 (RTEF-1) has been suggested to induce angiogenesis through regulating target genes. Whether RTEF-1 has a direct role in angiogenesis and what specific genes are involved in RTEF-1 driven angiogenisis have not been elucidated. We found that over-expressing RTEF-1 in Human dermal microvascular endothelial cells-1 (HMEC-1) significantly increased endothelial cell aggregation, growth and migration while the processes were inhibited by siRNA of RTEF-1. In addition, we observed that Endothelial differentiation gene-1 (Edg-1) expression was up-regulated by RTEF-1 at the transcriptional level. RTEF-1 could bind to Edg-1 promoter and subsequently induce its activity. Edg-1 siRNA significantly blocked RTEF-1-driven increases in endothelial cell aggregation in a Matrigel assay and retarded RTEF-1-induced endothelial cell growth and migration. Pertussis Toxin (PTX), a Gi/Go protein sensitive inhibitor, was found to inhibit RTEF-1 driven endothelial cell aggregation and migration. Our data demonstrates that Edg-1 is a potential target gene of RTEF-1 and is involved in RTEF-1-induced angiogenesis in endothelial cells. Gi/Go protein coupled receptor pathway plays a role in RTEF-1 driven angiogenesis in endothelial cells.

Characterization of the transcriptional activation domains of human TEF3-1 (transcription enhancer factor 3 isoform 1)

“…Above all, TEF3-1 is important for physiologically processes such as cell proliferation, cell migration, mammalian cell fate specification [32][33][34][35]. Recent paper mentioned that TEF3-1 is a prognostic marker for cancer progression and a potential target for cancer treatment [33,[36][37][38].…”

“…Recent paper mentioned that TEF3-1 is a prognostic marker for cancer progression and a potential target for cancer treatment [33,[36][37][38]. However, even in the angiogenesis of cancer, the molecular function of TEF3-1 protein is not fully understood.…”

Promoting endothelial function by S-nitrosoglutathione through the HIF-1α/VEGF pathway stimulates neurorepair and functional recovery following experimental stroke in rats

“…Matrigel assay was performed as described previously. 49 Briefly, bEnd3 endothelial cells were plated in a six-well Matrigel plate (BD Biocoat Matrigel Matrix, 354432). The cells were seeded at a density of 10 5 cells per well in 2 mL medium.…”

SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis

“…Cells were transfected with Lipofectamine 2000 (Invitrogen) according to the manufacturer's suggestions. Human dermal microvascular endothelial cells (HMEC-1) and Human umbilical vein endothelial cells (HUVEC) [ 30 , 31 ], which have endothelial characteristics of cell migration and tubular structure formation, were purchased from Science Research Laboratories (ScienCell Research Laboratories, San Diego, CA, USA) and stored by the lab of Institute of Biomedical Sciences and School of Life Sciences, East China Normal University. HMEC-1 cells were maintained in MCDB-131 medium (Invitrogen, Life Technologies) supplemented with 10% fetal bovine serum (FBS), 10 ng/mL of human epidermal growth factor (EGF) (Sigma, St. Louis, MO), 1 μg/mL of hydrocortisone, 5 mg/mL of L-glutamine, and antibiotics (penicillin-streptomycin) at 37°C with 5% CO 2 .…”